The utilization of an ocular wound chamber on corneal epithelial wounds

Jennifer S McDaniel,1 Andrew W Holt,1 Elaine D Por,1 Elof Eriksson,2 Anthony J Johnson,1 Gina L Griffith1 1Department of Sensory Trauma, United States Army Institute of Surgical Research, San Antonio, TX, USA; 2Harvard Medical School, Boston, MA, USA Purpose: Currently available ocular moisture ch...

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Autores principales:	McDaniel JS, Holt AW, Por ED, Eriksson E, Johnson AJ, Griffith GL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	corneal epithelial wound healing periocular burns wound chamber Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/d45f857ac1b14baab27a435b68402d5c
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spelling	oai:doaj.org-article:d45f857ac1b14baab27a435b68402d5c2021-12-02T01:10:20ZThe utilization of an ocular wound chamber on corneal epithelial wounds1177-5483https://doaj.org/article/d45f857ac1b14baab27a435b68402d5c2018-05-01T00:00:00Zhttps://www.dovepress.com/the-utilization-of-an-ocular-wound-chamber-on-corneal-epithelial-wound-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Jennifer S McDaniel,1 Andrew W Holt,1 Elaine D Por,1 Elof Eriksson,2 Anthony J Johnson,1 Gina L Griffith1 1Department of Sensory Trauma, United States Army Institute of Surgical Research, San Antonio, TX, USA; 2Harvard Medical School, Boston, MA, USA Purpose: Currently available ocular moisture chambers are not adequate to manage the treatment of periocular burns, corneal injuries, and infection. The purpose of these studies was to demonstrate that a flexible, semi-transparent ocular wound chamber device adapted from technology currently used on dermal wounds is safe for use on corneal epithelial injuries.Materials and methods: A depilatory cream (Nair™, 30 seconds) was utilized to remove the excess hair surrounding the left eyes of anesthetized Institute Armand Frappier (IAF) hairless, female guinea pigs (Crl:HA-Hrhr). A 4 mm corneal epithelium defect was created using a corneal rust ring remover (Algerbrush®II). Epithelial defects were either left untreated or the eyes were fitted with an ocular wound chamber and 0.5 mL of hydroxypropyl methylcellulose (HPMC) gel (GenTeal®) or HPMC liquid (GenTeal®) was injected into each chamber (N=5 per group). At 0, 24, 48, and 72 hours fluorescein and optical coherence tomography imaging was collected and the intraocular pressure (IOP) was measured. H&E staining was performed on corneal and eyelid skin samples and evaluated by a veterinary pathologist. Results: Corneal epithelial wounds demonstrated 100% closure rates when left untreated or treated with an ocular wound chamber containing HPMC gel at 72 hours while wounds treated with an ocular wound chamber containing HPMC liquid were 98% healed. No significant differences were found in corneal thickness and wound healing, IOP, or eyelid skin pathology in any treatment group when compared to controls.Conclusions: This study indicates that adapted wound chamber technology can be safely used on sterile, corneal epithelial wounds without adverse effects on periocular or ocular tissue when filled with a liquid or gel. Keywords: corneal epithelial wound healing, periocular burns, wound chamber, exposure keratopathy, guinea pigMcDaniel JSHolt AWPor EDEriksson EJohnson AJGriffith GLDove Medical Pressarticlecorneal epithelial wound healingperiocular burnswound chamberOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 12, Pp 903-911 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	corneal epithelial wound healing periocular burns wound chamber Ophthalmology RE1-994
spellingShingle	corneal epithelial wound healing periocular burns wound chamber Ophthalmology RE1-994 McDaniel JS Holt AW Por ED Eriksson E Johnson AJ Griffith GL The utilization of an ocular wound chamber on corneal epithelial wounds
description	Jennifer S McDaniel,1 Andrew W Holt,1 Elaine D Por,1 Elof Eriksson,2 Anthony J Johnson,1 Gina L Griffith1 1Department of Sensory Trauma, United States Army Institute of Surgical Research, San Antonio, TX, USA; 2Harvard Medical School, Boston, MA, USA Purpose: Currently available ocular moisture chambers are not adequate to manage the treatment of periocular burns, corneal injuries, and infection. The purpose of these studies was to demonstrate that a flexible, semi-transparent ocular wound chamber device adapted from technology currently used on dermal wounds is safe for use on corneal epithelial injuries.Materials and methods: A depilatory cream (Nair™, 30 seconds) was utilized to remove the excess hair surrounding the left eyes of anesthetized Institute Armand Frappier (IAF) hairless, female guinea pigs (Crl:HA-Hrhr). A 4 mm corneal epithelium defect was created using a corneal rust ring remover (Algerbrush®II). Epithelial defects were either left untreated or the eyes were fitted with an ocular wound chamber and 0.5 mL of hydroxypropyl methylcellulose (HPMC) gel (GenTeal®) or HPMC liquid (GenTeal®) was injected into each chamber (N=5 per group). At 0, 24, 48, and 72 hours fluorescein and optical coherence tomography imaging was collected and the intraocular pressure (IOP) was measured. H&E staining was performed on corneal and eyelid skin samples and evaluated by a veterinary pathologist. Results: Corneal epithelial wounds demonstrated 100% closure rates when left untreated or treated with an ocular wound chamber containing HPMC gel at 72 hours while wounds treated with an ocular wound chamber containing HPMC liquid were 98% healed. No significant differences were found in corneal thickness and wound healing, IOP, or eyelid skin pathology in any treatment group when compared to controls.Conclusions: This study indicates that adapted wound chamber technology can be safely used on sterile, corneal epithelial wounds without adverse effects on periocular or ocular tissue when filled with a liquid or gel. Keywords: corneal epithelial wound healing, periocular burns, wound chamber, exposure keratopathy, guinea pig
format	article
author	McDaniel JS Holt AW Por ED Eriksson E Johnson AJ Griffith GL
author_facet	McDaniel JS Holt AW Por ED Eriksson E Johnson AJ Griffith GL
author_sort	McDaniel JS
title	The utilization of an ocular wound chamber on corneal epithelial wounds
title_short	The utilization of an ocular wound chamber on corneal epithelial wounds
title_full	The utilization of an ocular wound chamber on corneal epithelial wounds
title_fullStr	The utilization of an ocular wound chamber on corneal epithelial wounds
title_full_unstemmed	The utilization of an ocular wound chamber on corneal epithelial wounds
title_sort	utilization of an ocular wound chamber on corneal epithelial wounds
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/d45f857ac1b14baab27a435b68402d5c
work_keys_str_mv	AT mcdanieljs theutilizationofanocularwoundchamberoncornealepithelialwounds AT holtaw theutilizationofanocularwoundchamberoncornealepithelialwounds AT pored theutilizationofanocularwoundchamberoncornealepithelialwounds AT erikssone theutilizationofanocularwoundchamberoncornealepithelialwounds AT johnsonaj theutilizationofanocularwoundchamberoncornealepithelialwounds AT griffithgl theutilizationofanocularwoundchamberoncornealepithelialwounds AT mcdanieljs utilizationofanocularwoundchamberoncornealepithelialwounds AT holtaw utilizationofanocularwoundchamberoncornealepithelialwounds AT pored utilizationofanocularwoundchamberoncornealepithelialwounds AT erikssone utilizationofanocularwoundchamberoncornealepithelialwounds AT johnsonaj utilizationofanocularwoundchamberoncornealepithelialwounds AT griffithgl utilizationofanocularwoundchamberoncornealepithelialwounds
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The utilization of an ocular wound chamber on corneal epithelial wounds

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