Engineered Newcastle disease virus expressing the F and G proteins of AMPV-C confers protection against challenges in turkeys

Abstract Avian metapneumovirus (AMPV) infects the respiratory and reproductive tracts of domestic poultry, resulting in substantial economic losses for producers. Live attenuated vaccines appear to be the most effective in countries where the disease is prevalent. However, reversion to virulence has...

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Autores principales:	Haixia Hu, Jason P. Roth, Laszlo Zsak, Qingzhong Yu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d46242ac641244578f0315c8396bfc15
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spelling	oai:doaj.org-article:d46242ac641244578f0315c8396bfc152021-12-02T16:06:14ZEngineered Newcastle disease virus expressing the F and G proteins of AMPV-C confers protection against challenges in turkeys10.1038/s41598-017-04267-72045-2322https://doaj.org/article/d46242ac641244578f0315c8396bfc152017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04267-7https://doaj.org/toc/2045-2322Abstract Avian metapneumovirus (AMPV) infects the respiratory and reproductive tracts of domestic poultry, resulting in substantial economic losses for producers. Live attenuated vaccines appear to be the most effective in countries where the disease is prevalent. However, reversion to virulence has been demonstrated in several studies. Therefore, the development of a stable and safe next generation vaccine against the AMPV disease is needed. In the present study, we generated a recombinant Newcastle disease virus (NDV) vectoring the fusion (F) protein and glycoprotein (G) genes of AMPV subtype-C (AMPV-C) as a bivalent vaccine candidate using reverse genetics technology. The recombinant virus, rLS/AMPV-C F&G, was slightly attenuated in vivo, yet maintained similar characteristics in vitro when compared to the parental LaSota virus. Vaccination of turkeys with rLS/AMPV-C F&G induced both AMPV-C and NDV-specific antibody responses, and provided significant protection against pathogenic AMPV-C challenge and complete protection against velogenic NDV challenge. These results suggest that the rLS/AMPV-C F&G recombinant virus is a safe and effective bivalent vaccine candidate and that the expression of both F and G proteins of AMPV-C induces a protective response against the AMPV-C disease.Haixia HuJason P. RothLaszlo ZsakQingzhong YuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haixia Hu Jason P. Roth Laszlo Zsak Qingzhong Yu Engineered Newcastle disease virus expressing the F and G proteins of AMPV-C confers protection against challenges in turkeys
description	Abstract Avian metapneumovirus (AMPV) infects the respiratory and reproductive tracts of domestic poultry, resulting in substantial economic losses for producers. Live attenuated vaccines appear to be the most effective in countries where the disease is prevalent. However, reversion to virulence has been demonstrated in several studies. Therefore, the development of a stable and safe next generation vaccine against the AMPV disease is needed. In the present study, we generated a recombinant Newcastle disease virus (NDV) vectoring the fusion (F) protein and glycoprotein (G) genes of AMPV subtype-C (AMPV-C) as a bivalent vaccine candidate using reverse genetics technology. The recombinant virus, rLS/AMPV-C F&G, was slightly attenuated in vivo, yet maintained similar characteristics in vitro when compared to the parental LaSota virus. Vaccination of turkeys with rLS/AMPV-C F&G induced both AMPV-C and NDV-specific antibody responses, and provided significant protection against pathogenic AMPV-C challenge and complete protection against velogenic NDV challenge. These results suggest that the rLS/AMPV-C F&G recombinant virus is a safe and effective bivalent vaccine candidate and that the expression of both F and G proteins of AMPV-C induces a protective response against the AMPV-C disease.
format	article
author	Haixia Hu Jason P. Roth Laszlo Zsak Qingzhong Yu
author_facet	Haixia Hu Jason P. Roth Laszlo Zsak Qingzhong Yu
author_sort	Haixia Hu
title	Engineered Newcastle disease virus expressing the F and G proteins of AMPV-C confers protection against challenges in turkeys
title_short	Engineered Newcastle disease virus expressing the F and G proteins of AMPV-C confers protection against challenges in turkeys
title_full	Engineered Newcastle disease virus expressing the F and G proteins of AMPV-C confers protection against challenges in turkeys
title_fullStr	Engineered Newcastle disease virus expressing the F and G proteins of AMPV-C confers protection against challenges in turkeys
title_full_unstemmed	Engineered Newcastle disease virus expressing the F and G proteins of AMPV-C confers protection against challenges in turkeys
title_sort	engineered newcastle disease virus expressing the f and g proteins of ampv-c confers protection against challenges in turkeys
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d46242ac641244578f0315c8396bfc15
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Engineered Newcastle disease virus expressing the F and G proteins of AMPV-C confers protection against challenges in turkeys

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