High levels of AAV vector integration into CRISPR-induced DNA breaks

In-depth characterization of adeno-associated virus (AAV)-mediated CRISPR delivery is still lacking. Here, the authors show high levels of integration into Cas9-induced double-strand breaks (DSBs) in therapeutically relevant genes in vivo.

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Autores principales:	Killian S. Hanlon, Benjamin P. Kleinstiver, Sara P. Garcia, Mikołaj P. Zaborowski, Adrienn Volak, Stefan E. Spirig, Alissa Muller, Alexander A. Sousa, Shengdar Q. Tsai, Niclas E. Bengtsson, Camilla Lööv, Martin Ingelsson, Jeffrey S. Chamberlain, David P. Corey, Martin J. Aryee, J. Keith Joung, Xandra O. Breakefield, Casey A. Maguire, Bence György
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/d463e02a3bdc40c4aca343f4b409877a
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spelling	oai:doaj.org-article:d463e02a3bdc40c4aca343f4b409877a2021-12-02T16:57:50ZHigh levels of AAV vector integration into CRISPR-induced DNA breaks10.1038/s41467-019-12449-22041-1723https://doaj.org/article/d463e02a3bdc40c4aca343f4b409877a2019-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12449-2https://doaj.org/toc/2041-1723In-depth characterization of adeno-associated virus (AAV)-mediated CRISPR delivery is still lacking. Here, the authors show high levels of integration into Cas9-induced double-strand breaks (DSBs) in therapeutically relevant genes in vivo.Killian S. HanlonBenjamin P. KleinstiverSara P. GarciaMikołaj P. ZaborowskiAdrienn VolakStefan E. SpirigAlissa MullerAlexander A. SousaShengdar Q. TsaiNiclas E. BengtssonCamilla LöövMartin IngelssonJeffrey S. ChamberlainDavid P. CoreyMartin J. AryeeJ. Keith JoungXandra O. BreakefieldCasey A. MaguireBence GyörgyNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Killian S. Hanlon Benjamin P. Kleinstiver Sara P. Garcia Mikołaj P. Zaborowski Adrienn Volak Stefan E. Spirig Alissa Muller Alexander A. Sousa Shengdar Q. Tsai Niclas E. Bengtsson Camilla Lööv Martin Ingelsson Jeffrey S. Chamberlain David P. Corey Martin J. Aryee J. Keith Joung Xandra O. Breakefield Casey A. Maguire Bence György High levels of AAV vector integration into CRISPR-induced DNA breaks
description	In-depth characterization of adeno-associated virus (AAV)-mediated CRISPR delivery is still lacking. Here, the authors show high levels of integration into Cas9-induced double-strand breaks (DSBs) in therapeutically relevant genes in vivo.
format	article
author	Killian S. Hanlon Benjamin P. Kleinstiver Sara P. Garcia Mikołaj P. Zaborowski Adrienn Volak Stefan E. Spirig Alissa Muller Alexander A. Sousa Shengdar Q. Tsai Niclas E. Bengtsson Camilla Lööv Martin Ingelsson Jeffrey S. Chamberlain David P. Corey Martin J. Aryee J. Keith Joung Xandra O. Breakefield Casey A. Maguire Bence György
author_facet	Killian S. Hanlon Benjamin P. Kleinstiver Sara P. Garcia Mikołaj P. Zaborowski Adrienn Volak Stefan E. Spirig Alissa Muller Alexander A. Sousa Shengdar Q. Tsai Niclas E. Bengtsson Camilla Lööv Martin Ingelsson Jeffrey S. Chamberlain David P. Corey Martin J. Aryee J. Keith Joung Xandra O. Breakefield Casey A. Maguire Bence György
author_sort	Killian S. Hanlon
title	High levels of AAV vector integration into CRISPR-induced DNA breaks
title_short	High levels of AAV vector integration into CRISPR-induced DNA breaks
title_full	High levels of AAV vector integration into CRISPR-induced DNA breaks
title_fullStr	High levels of AAV vector integration into CRISPR-induced DNA breaks
title_full_unstemmed	High levels of AAV vector integration into CRISPR-induced DNA breaks
title_sort	high levels of aav vector integration into crispr-induced dna breaks
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/d463e02a3bdc40c4aca343f4b409877a
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High levels of AAV vector integration into CRISPR-induced DNA breaks

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