Phylogeny-based comparative analysis of venom proteome variation in a clade of rattlesnakes (Sistrurus sp.).

A long-standing question in evolutionary studies of snake venoms is the extent to which phylogenetic divergence and diet can account for between-species differences in venom composition. Here we apply phylogeny-based comparative methods to address this question. We use data on venom variation genera...

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Autores principales: H Lisle Gibbs, Libia Sanz, Michael G Sovic, Juan J Calvete
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Publicado: Public Library of Science (PLoS) 2013
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spelling oai:doaj.org-article:d46a755e93db4a74a3ed1486b264d8002021-11-18T07:40:19ZPhylogeny-based comparative analysis of venom proteome variation in a clade of rattlesnakes (Sistrurus sp.).1932-620310.1371/journal.pone.0067220https://doaj.org/article/d46a755e93db4a74a3ed1486b264d8002013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23826238/?tool=EBIhttps://doaj.org/toc/1932-6203A long-standing question in evolutionary studies of snake venoms is the extent to which phylogenetic divergence and diet can account for between-species differences in venom composition. Here we apply phylogeny-based comparative methods to address this question. We use data on venom variation generated using proteomic techniques for all members of a small clade of rattlesnakes (Sistrurus sp.) and two outgroups for which phylogenetic and diet information is available. We first complete the characterization of venom variation for all members of this clade with a "venomic" analysis of pooled venoms from two members of this genus, S. milarius streckeri and S. m. milarius. These venoms exhibit the same general classes of proteins as those found in other Sistrurus species but differ in their relative abundances of specific protein families. We then test whether there is significant phylogenetic signal in the relative abundances of major venom proteins across species and if diet (measured as percent mammals and lizards among all prey consumed) covaries with venom composition after phylogenetic divergence is accounted for. We found no evidence for significant phylogenetic signal in venom variation: K values for seven snake venom proteins and two composite venom variables [PC 1 and 2]) were all nonsignificant and lower (mean = 0.11+0.06 sd) than mean K values (>0.35) previously reported for a wide range of morphological, life history, physiological and behavioral traits from other species. Finally, analyses based on Phylogenetic Generalized Least Squares (PGLS) methods reveal that variation in abundance of some venom proteins, most strongly CRISP is significantly related to snake diet. Our results demonstrate that venom variation in these snakes is evolutionarily a highly labile trait even among very closely-related taxa and that natural selection acting through diet variation may play a role in molding the relative abundance of specific venom proteins.H Lisle GibbsLibia SanzMichael G SovicJuan J CalvetePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e67220 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
H Lisle Gibbs
Libia Sanz
Michael G Sovic
Juan J Calvete
Phylogeny-based comparative analysis of venom proteome variation in a clade of rattlesnakes (Sistrurus sp.).
description A long-standing question in evolutionary studies of snake venoms is the extent to which phylogenetic divergence and diet can account for between-species differences in venom composition. Here we apply phylogeny-based comparative methods to address this question. We use data on venom variation generated using proteomic techniques for all members of a small clade of rattlesnakes (Sistrurus sp.) and two outgroups for which phylogenetic and diet information is available. We first complete the characterization of venom variation for all members of this clade with a "venomic" analysis of pooled venoms from two members of this genus, S. milarius streckeri and S. m. milarius. These venoms exhibit the same general classes of proteins as those found in other Sistrurus species but differ in their relative abundances of specific protein families. We then test whether there is significant phylogenetic signal in the relative abundances of major venom proteins across species and if diet (measured as percent mammals and lizards among all prey consumed) covaries with venom composition after phylogenetic divergence is accounted for. We found no evidence for significant phylogenetic signal in venom variation: K values for seven snake venom proteins and two composite venom variables [PC 1 and 2]) were all nonsignificant and lower (mean = 0.11+0.06 sd) than mean K values (>0.35) previously reported for a wide range of morphological, life history, physiological and behavioral traits from other species. Finally, analyses based on Phylogenetic Generalized Least Squares (PGLS) methods reveal that variation in abundance of some venom proteins, most strongly CRISP is significantly related to snake diet. Our results demonstrate that venom variation in these snakes is evolutionarily a highly labile trait even among very closely-related taxa and that natural selection acting through diet variation may play a role in molding the relative abundance of specific venom proteins.
format article
author H Lisle Gibbs
Libia Sanz
Michael G Sovic
Juan J Calvete
author_facet H Lisle Gibbs
Libia Sanz
Michael G Sovic
Juan J Calvete
author_sort H Lisle Gibbs
title Phylogeny-based comparative analysis of venom proteome variation in a clade of rattlesnakes (Sistrurus sp.).
title_short Phylogeny-based comparative analysis of venom proteome variation in a clade of rattlesnakes (Sistrurus sp.).
title_full Phylogeny-based comparative analysis of venom proteome variation in a clade of rattlesnakes (Sistrurus sp.).
title_fullStr Phylogeny-based comparative analysis of venom proteome variation in a clade of rattlesnakes (Sistrurus sp.).
title_full_unstemmed Phylogeny-based comparative analysis of venom proteome variation in a clade of rattlesnakes (Sistrurus sp.).
title_sort phylogeny-based comparative analysis of venom proteome variation in a clade of rattlesnakes (sistrurus sp.).
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/d46a755e93db4a74a3ed1486b264d800
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AT michaelgsovic phylogenybasedcomparativeanalysisofvenomproteomevariationinacladeofrattlesnakessistrurussp
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