The Effect of Dragon Fruit Extract on Experimental Mesentery Arterial Ischemia-Reperfusion in Rats
We hypothesized that dragon fruit extract (DFE) might be a protective agent in oxidative stress damage that develops against ischemia and reperfusion injury in the rat intestine. The rats used in the study were randomly divided into 8 groups, with 6 animals in each group. The HEALTHY group was not i...
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University of Kafkas
2021
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oai:doaj.org-article:d46d6d0bcff34c73b2e209bffbc71ad92021-11-03T20:10:00ZThe Effect of Dragon Fruit Extract on Experimental Mesentery Arterial Ischemia-Reperfusion in Rats10.9775/kvfd.2021.258801300-60451309-2251https://doaj.org/article/d46d6d0bcff34c73b2e209bffbc71ad92021-10-01T00:00:00Zhttps://vetdergikafkas.org/uploads/pdf/pdf_KVFD_2830.pdfhttps://doaj.org/toc/1300-6045https://doaj.org/toc/1309-2251We hypothesized that dragon fruit extract (DFE) might be a protective agent in oxidative stress damage that develops against ischemia and reperfusion injury in the rat intestine. The rats used in the study were randomly divided into 8 groups, with 6 animals in each group. The HEALTHY group was not induced by ischemia and not given DFE (Group 1). The DFE1000 group was not induced by ischemia; however, DFE was given 1000 mg/kg (Group 2). Ischemia was induced for 1 h in groups 3, 4, 5, 6, 7 and 8. The clamps were then removed to allow reperfusion for 45 min (Groups 6, 7 and 8). DFE was given to rats at doses of 500mg/kg and 1000 mg/kg 30 min before Ischemia (I) and Ischemia/Reperfusion (I/R) administration. At the end of the experiment, histopathological, biochemical and molecular analyses were performed on the intestinal tissues. While glutathione peroxidase, superoxide dismutase, glutathione levels increased significantly in the I+DFE500, I+DFE1000, I/R+DFE500 and I/ R+DFE1000 groups compared to the I and I/R groups, there was a significant decrease in tumor necrosis factor-α, Caspase 3 and malondialdehyde (P<0.05). In addition, the loss of tissue integrity, and the increase of inflammatory cells were decreased in the treatment groups. As a result, it was determined that DFE has a strong protective role against oxidative damage in the treatment of intestinal ischemia-reperfusion injury.Levent SAHINErdem TOKTAYMuhammed YAYLAPınar AKSU KILICLEAhmet HARMANKAYACem OZICSerdar YIGITNevra AYDEMIR CELEPUniversity of Kafkasarticledragon fruit extractintestineischemia/reperfusionoxidative stressratVeterinary medicineSF600-1100ENKafkas Universitesi Veteriner Fakultesi Dergisi, Vol 27 , Iss 5, Pp 575-581 (2021) |
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| topic |
dragon fruit extract intestine ischemia/reperfusion oxidative stress rat Veterinary medicine SF600-1100 |
| spellingShingle |
dragon fruit extract intestine ischemia/reperfusion oxidative stress rat Veterinary medicine SF600-1100 Levent SAHIN Erdem TOKTAY Muhammed YAYLA Pınar AKSU KILICLE Ahmet HARMANKAYA Cem OZIC Serdar YIGIT Nevra AYDEMIR CELEP The Effect of Dragon Fruit Extract on Experimental Mesentery Arterial Ischemia-Reperfusion in Rats |
| description |
We hypothesized that dragon fruit extract (DFE) might be a protective agent in oxidative stress damage that develops against ischemia and reperfusion injury in the rat intestine. The rats used in the study were randomly divided into 8 groups, with 6 animals in each group. The HEALTHY group was not induced by ischemia and not given DFE (Group 1). The DFE1000 group was not induced by ischemia; however, DFE was given 1000 mg/kg (Group 2). Ischemia was induced for 1 h in groups 3, 4, 5, 6, 7 and 8. The clamps were then removed to allow reperfusion for 45 min (Groups 6, 7 and 8). DFE was given to rats at doses of 500mg/kg and 1000 mg/kg 30 min before Ischemia (I) and Ischemia/Reperfusion (I/R) administration. At the end of the experiment, histopathological, biochemical and molecular analyses were performed on the intestinal tissues. While glutathione peroxidase, superoxide dismutase, glutathione levels increased significantly in the I+DFE500, I+DFE1000, I/R+DFE500 and I/ R+DFE1000 groups compared to the I and I/R groups, there was a significant decrease in tumor necrosis factor-α, Caspase 3 and malondialdehyde (P<0.05). In addition, the loss of tissue integrity, and the increase of inflammatory cells were decreased in the treatment groups. As a result, it was determined that DFE has a strong protective role against oxidative damage in the treatment of intestinal ischemia-reperfusion injury. |
| format |
article |
| author |
Levent SAHIN Erdem TOKTAY Muhammed YAYLA Pınar AKSU KILICLE Ahmet HARMANKAYA Cem OZIC Serdar YIGIT Nevra AYDEMIR CELEP |
| author_facet |
Levent SAHIN Erdem TOKTAY Muhammed YAYLA Pınar AKSU KILICLE Ahmet HARMANKAYA Cem OZIC Serdar YIGIT Nevra AYDEMIR CELEP |
| author_sort |
Levent SAHIN |
| title |
The Effect of Dragon Fruit Extract on Experimental Mesentery Arterial Ischemia-Reperfusion in Rats |
| title_short |
The Effect of Dragon Fruit Extract on Experimental Mesentery Arterial Ischemia-Reperfusion in Rats |
| title_full |
The Effect of Dragon Fruit Extract on Experimental Mesentery Arterial Ischemia-Reperfusion in Rats |
| title_fullStr |
The Effect of Dragon Fruit Extract on Experimental Mesentery Arterial Ischemia-Reperfusion in Rats |
| title_full_unstemmed |
The Effect of Dragon Fruit Extract on Experimental Mesentery Arterial Ischemia-Reperfusion in Rats |
| title_sort |
effect of dragon fruit extract on experimental mesentery arterial ischemia-reperfusion in rats |
| publisher |
University of Kafkas |
| publishDate |
2021 |
| url |
https://doaj.org/article/d46d6d0bcff34c73b2e209bffbc71ad9 |
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