Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions

Abstract. Dermiyati, Suharjo R, Telaumbanua M, Yosita R, Sari AW, Andayani AP. 2020. Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions. Biodiversitas 21: 4213-4220. Increased production of...

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Autores principales: DERMIYATI DERMIYATI, RADIX SUHARJO, MARELI TELAUMBANUA, RULLY YOSITA, ANGGI WINANDA SARI, ANIS PUJI ANDAYANI
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spelling oai:doaj.org-article:d46db688c81f4158a64a4045cda4a3432021-11-22T00:41:43ZAbundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions1412-033X2085-472210.13057/biodiv/d210936https://doaj.org/article/d46db688c81f4158a64a4045cda4a3432020-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6434https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Dermiyati, Suharjo R, Telaumbanua M, Yosita R, Sari AW, Andayani AP. 2020. Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions. Biodiversitas 21: 4213-4220. Increased production of palm oil causes an increase in oil palm empty fruit bunches waste, while the utilization of the waste has not been carried out optimally. Oil palm empty fruit bunches contain high cellulose content which promotes the development of various types of bacteria with different characteristics. This study was aimed to determine the abundance and the characterization of bacteria that were isolated from extract suspension of oil palm empty fruit bunches in the form of a local microorganism solution under aerobic, anaerobic, and facultative anaerobic conditions. For isolation, the oil palm empty fruit extract suspension was inoculated on media Plate Count Agar Peptone and bacterial abundance was determined. Various bacterial characterization test included bacterial morphology test in the form of color and shape of macroscopic isolates, gram test using KOH 3%, fermentative oxidative test, soft rot test on potato tubers, hypersensitivity test on tobacco plant leaves, and hypo virulent test which was indicated by the presence of symptoms of disease in cucumber sprouts and indicated in the disease severity index (DSI) was carried out. The results of local microorganism solution isolation from oil palm empty fruit bunches obtained as many as 220 bacterial isolates consisting of 84 isolates in aerobic condition, 68 isolates in anaerobic condition, and 68 isolates in facultative anaerobic condition. The highest bacterial abundance in aerobic conditions was equal to 285.60 x 1010 CFU mL-1. Each bacterial isolate had different characteristics. Most of the characteristics of bacteria were turbid white (34.55%), round (69.55%), gram-positive (76.36%), fermentative (89.55%), softrot negative (71.82%), hypersensitive negative (97.27%), and virulent (74.55%).DERMIYATI DERMIYATIRADIX SUHARJOMARELI TELAUMBANUARULLY YOSITAANGGI WINANDA SARIANIS PUJI ANDAYANIMBI & UNS Soloarticleabundance, bacteria, characteristics, local microorganismsBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 9 (2020)
institution DOAJ
collection DOAJ
language EN
topic abundance, bacteria, characteristics, local microorganisms
Biology (General)
QH301-705.5
spellingShingle abundance, bacteria, characteristics, local microorganisms
Biology (General)
QH301-705.5
DERMIYATI DERMIYATI
RADIX SUHARJO
MARELI TELAUMBANUA
RULLY YOSITA
ANGGI WINANDA SARI
ANIS PUJI ANDAYANI
Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions
description Abstract. Dermiyati, Suharjo R, Telaumbanua M, Yosita R, Sari AW, Andayani AP. 2020. Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions. Biodiversitas 21: 4213-4220. Increased production of palm oil causes an increase in oil palm empty fruit bunches waste, while the utilization of the waste has not been carried out optimally. Oil palm empty fruit bunches contain high cellulose content which promotes the development of various types of bacteria with different characteristics. This study was aimed to determine the abundance and the characterization of bacteria that were isolated from extract suspension of oil palm empty fruit bunches in the form of a local microorganism solution under aerobic, anaerobic, and facultative anaerobic conditions. For isolation, the oil palm empty fruit extract suspension was inoculated on media Plate Count Agar Peptone and bacterial abundance was determined. Various bacterial characterization test included bacterial morphology test in the form of color and shape of macroscopic isolates, gram test using KOH 3%, fermentative oxidative test, soft rot test on potato tubers, hypersensitivity test on tobacco plant leaves, and hypo virulent test which was indicated by the presence of symptoms of disease in cucumber sprouts and indicated in the disease severity index (DSI) was carried out. The results of local microorganism solution isolation from oil palm empty fruit bunches obtained as many as 220 bacterial isolates consisting of 84 isolates in aerobic condition, 68 isolates in anaerobic condition, and 68 isolates in facultative anaerobic condition. The highest bacterial abundance in aerobic conditions was equal to 285.60 x 1010 CFU mL-1. Each bacterial isolate had different characteristics. Most of the characteristics of bacteria were turbid white (34.55%), round (69.55%), gram-positive (76.36%), fermentative (89.55%), softrot negative (71.82%), hypersensitive negative (97.27%), and virulent (74.55%).
format article
author DERMIYATI DERMIYATI
RADIX SUHARJO
MARELI TELAUMBANUA
RULLY YOSITA
ANGGI WINANDA SARI
ANIS PUJI ANDAYANI
author_facet DERMIYATI DERMIYATI
RADIX SUHARJO
MARELI TELAUMBANUA
RULLY YOSITA
ANGGI WINANDA SARI
ANIS PUJI ANDAYANI
author_sort DERMIYATI DERMIYATI
title Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions
title_short Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions
title_full Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions
title_fullStr Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions
title_full_unstemmed Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions
title_sort abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions
publisher MBI & UNS Solo
publishDate 2020
url https://doaj.org/article/d46db688c81f4158a64a4045cda4a343
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