Genotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 2019

ABSTRACT: Objectives: This study assessed the incidence and resistance of Mycoplasma pneumoniae (MP) in children in Qingdao, China, in 2019. Methods: We detected MP infection in 78 pharyngeal swabs from children with pneumonia by qPCR. The RepMP4 element in the P1 adhesin gene, domain V of the 23S...

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Autores principales:	Fa-Chun Jiang, Rui-Fang Wang, Ping Chen, Li-Yan Dong, Xia Wang, Qin Song, Yi-Qiu Wan, Qin-Qin Song, Juan Song, Yan-Hai Wang, Zhi-Qiang Xia, Dong Xia, Jun Han
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Mycoplasma pneumoniae Pneumonia Children Macrolide resistance Drug resistance gene Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d46e5a5868824dd98ced62b99580c94e
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spelling	oai:doaj.org-article:d46e5a5868824dd98ced62b99580c94e2021-11-18T04:48:30ZGenotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 20192213-716510.1016/j.jgar.2021.10.003https://doaj.org/article/d46e5a5868824dd98ced62b99580c94e2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2213716521002186https://doaj.org/toc/2213-7165ABSTRACT: Objectives: This study assessed the incidence and resistance of Mycoplasma pneumoniae (MP) in children in Qingdao, China, in 2019. Methods: We detected MP infection in 78 pharyngeal swabs from children with pneumonia by qPCR. The RepMP4 element in the P1 adhesin gene, domain V of the 23S rRNA gene, and the L4/L22 ribosomal proteins were amplified by nested PCR. Evolutionary analysis was conducted based on the P1 gene sequence. Resistance mutations in domain V of the 23S rRNA gene and L4/L22 ribosomal proteins were analysed. Results: The incidence of MP infection in children with pneumonia was 59.0% (46/78). The mean duration of MP infection was longer than that of non-MP infection. According to P1 gene sequencing of 21 samples, 12 (57.1%) were type 1 and 9 (42.9%) were type 2. Drug resistance mutations A2063G in domain V of 23S rRNA gene and T508C in L22 were identified from all sequenced MP. However, mutations at positions 2064 and 2617 were not found in this study. C162A mutation appeared in most type 2 samples. A430G mutation appeared in one type 1 sample and in several type 2 samples. T279C mutation in L22 was mostly found in type 2 samples. Conclusion: The incidence of MP infection was 59.0% in children with pneumonia in Qingdao in 2019. Type 1 MP infection was slightly more common than type 2, indicating that the genotype of MP is gradually shifting from type 1 to type 2. Macrolide resistance mutation A2063G could be detected in all sequenced MP.Fa-Chun JiangRui-Fang WangPing ChenLi-Yan DongXia WangQin SongYi-Qiu WanQin-Qin SongJuan SongYan-Hai WangZhi-Qiang XiaDong XiaJun HanElsevierarticleMycoplasma pneumoniaePneumoniaChildrenMacrolide resistanceDrug resistance geneMicrobiologyQR1-502ENJournal of Global Antimicrobial Resistance, Vol 27, Iss , Pp 273-278 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mycoplasma pneumoniae Pneumonia Children Macrolide resistance Drug resistance gene Microbiology QR1-502
spellingShingle	Mycoplasma pneumoniae Pneumonia Children Macrolide resistance Drug resistance gene Microbiology QR1-502 Fa-Chun Jiang Rui-Fang Wang Ping Chen Li-Yan Dong Xia Wang Qin Song Yi-Qiu Wan Qin-Qin Song Juan Song Yan-Hai Wang Zhi-Qiang Xia Dong Xia Jun Han Genotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 2019
description	ABSTRACT: Objectives: This study assessed the incidence and resistance of Mycoplasma pneumoniae (MP) in children in Qingdao, China, in 2019. Methods: We detected MP infection in 78 pharyngeal swabs from children with pneumonia by qPCR. The RepMP4 element in the P1 adhesin gene, domain V of the 23S rRNA gene, and the L4/L22 ribosomal proteins were amplified by nested PCR. Evolutionary analysis was conducted based on the P1 gene sequence. Resistance mutations in domain V of the 23S rRNA gene and L4/L22 ribosomal proteins were analysed. Results: The incidence of MP infection in children with pneumonia was 59.0% (46/78). The mean duration of MP infection was longer than that of non-MP infection. According to P1 gene sequencing of 21 samples, 12 (57.1%) were type 1 and 9 (42.9%) were type 2. Drug resistance mutations A2063G in domain V of 23S rRNA gene and T508C in L22 were identified from all sequenced MP. However, mutations at positions 2064 and 2617 were not found in this study. C162A mutation appeared in most type 2 samples. A430G mutation appeared in one type 1 sample and in several type 2 samples. T279C mutation in L22 was mostly found in type 2 samples. Conclusion: The incidence of MP infection was 59.0% in children with pneumonia in Qingdao in 2019. Type 1 MP infection was slightly more common than type 2, indicating that the genotype of MP is gradually shifting from type 1 to type 2. Macrolide resistance mutation A2063G could be detected in all sequenced MP.
format	article
author	Fa-Chun Jiang Rui-Fang Wang Ping Chen Li-Yan Dong Xia Wang Qin Song Yi-Qiu Wan Qin-Qin Song Juan Song Yan-Hai Wang Zhi-Qiang Xia Dong Xia Jun Han
author_facet	Fa-Chun Jiang Rui-Fang Wang Ping Chen Li-Yan Dong Xia Wang Qin Song Yi-Qiu Wan Qin-Qin Song Juan Song Yan-Hai Wang Zhi-Qiang Xia Dong Xia Jun Han
author_sort	Fa-Chun Jiang
title	Genotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 2019
title_short	Genotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 2019
title_full	Genotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 2019
title_fullStr	Genotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 2019
title_full_unstemmed	Genotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 2019
title_sort	genotype and mutation patterns of macrolide resistance genes of mycoplasma pneumoniae from children with pneumonia in qingdao, china, in 2019
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/d46e5a5868824dd98ced62b99580c94e
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Genotype and mutation patterns of macrolide resistance genes of Mycoplasma pneumoniae from children with pneumonia in Qingdao, China, in 2019

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