Proteomic approach: Identification of Nephotettix virescens vector protein transmitting the tungro virus in rice

Abstract. Senoaji W, Rahardjo BT, Tarno H. 2021. Proteomic approach: Identification of Nephotettix virescens vector protein transmitting the tungro virus in rice. Biodiversitas 22: 2750-2755. As a vector of tungro virus, Nephotettix virescens is one primary pest in rice cultivars. Associated Rice Tu...

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Autores principales: WASIS SENOAJI, Bambang Tri Rahardjo, Hagus Tarno
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Publicado: MBI & UNS Solo 2021
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spelling oai:doaj.org-article:d46ef37be8f14a2f9316a7886144b2d72021-11-22T12:13:35ZProteomic approach: Identification of Nephotettix virescens vector protein transmitting the tungro virus in rice1412-033X2085-472210.13057/biodiv/d220537https://doaj.org/article/d46ef37be8f14a2f9316a7886144b2d72021-05-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7301https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Senoaji W, Rahardjo BT, Tarno H. 2021. Proteomic approach: Identification of Nephotettix virescens vector protein transmitting the tungro virus in rice. Biodiversitas 22: 2750-2755. As a vector of tungro virus, Nephotettix virescens is one primary pest in rice cultivars. Associated Rice Tungro Spherical Virus provides a helper component for Rice Tungro Bacilliform Virus acquisition produced the severe symptoms in rice. The vector proteins encoded by the virus become a poorly understood transmission of plant viruses, particularly in semi-persistent mechanisms. It is essential to develop new molecules to interrupt the transmission mechanism of plant viruses by insect vectors. This study aimed to detect the helper proteins in vectors with proteomics. Protein separation was performed using SDS-PAGE, and then was identified by the Liquid Chromatography-Mass Spectrometry (LC-MS/MS) to find the candidate proteins. The result showed that actin was identified in N. virescens as responsible protein that related to transmitting tungro viruses into the plant.WASIS SENOAJIBambang Tri RahardjoHagus TarnoMBI & UNS Soloarticlenephotettix virescens, protein, tungro, sds-page, ms/ms, actinBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 5 (2021)
institution DOAJ
collection DOAJ
language EN
topic nephotettix virescens, protein, tungro, sds-page, ms/ms, actin
Biology (General)
QH301-705.5
spellingShingle nephotettix virescens, protein, tungro, sds-page, ms/ms, actin
Biology (General)
QH301-705.5
WASIS SENOAJI
Bambang Tri Rahardjo
Hagus Tarno
Proteomic approach: Identification of Nephotettix virescens vector protein transmitting the tungro virus in rice
description Abstract. Senoaji W, Rahardjo BT, Tarno H. 2021. Proteomic approach: Identification of Nephotettix virescens vector protein transmitting the tungro virus in rice. Biodiversitas 22: 2750-2755. As a vector of tungro virus, Nephotettix virescens is one primary pest in rice cultivars. Associated Rice Tungro Spherical Virus provides a helper component for Rice Tungro Bacilliform Virus acquisition produced the severe symptoms in rice. The vector proteins encoded by the virus become a poorly understood transmission of plant viruses, particularly in semi-persistent mechanisms. It is essential to develop new molecules to interrupt the transmission mechanism of plant viruses by insect vectors. This study aimed to detect the helper proteins in vectors with proteomics. Protein separation was performed using SDS-PAGE, and then was identified by the Liquid Chromatography-Mass Spectrometry (LC-MS/MS) to find the candidate proteins. The result showed that actin was identified in N. virescens as responsible protein that related to transmitting tungro viruses into the plant.
format article
author WASIS SENOAJI
Bambang Tri Rahardjo
Hagus Tarno
author_facet WASIS SENOAJI
Bambang Tri Rahardjo
Hagus Tarno
author_sort WASIS SENOAJI
title Proteomic approach: Identification of Nephotettix virescens vector protein transmitting the tungro virus in rice
title_short Proteomic approach: Identification of Nephotettix virescens vector protein transmitting the tungro virus in rice
title_full Proteomic approach: Identification of Nephotettix virescens vector protein transmitting the tungro virus in rice
title_fullStr Proteomic approach: Identification of Nephotettix virescens vector protein transmitting the tungro virus in rice
title_full_unstemmed Proteomic approach: Identification of Nephotettix virescens vector protein transmitting the tungro virus in rice
title_sort proteomic approach: identification of nephotettix virescens vector protein transmitting the tungro virus in rice
publisher MBI & UNS Solo
publishDate 2021
url https://doaj.org/article/d46ef37be8f14a2f9316a7886144b2d7
work_keys_str_mv AT wasissenoaji proteomicapproachidentificationofnephotettixvirescensvectorproteintransmittingthetungrovirusinrice
AT bambangtrirahardjo proteomicapproachidentificationofnephotettixvirescensvectorproteintransmittingthetungrovirusinrice
AT hagustarno proteomicapproachidentificationofnephotettixvirescensvectorproteintransmittingthetungrovirusinrice
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