MicroRNA-4423-3p inhibits proliferation of fibroblast-like synoviocytes by targeting matrix metalloproteinase 13 in rheumatoid arthritis

Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease that is increasing in incidence worldwide. RA is regulated by a variety of microRNAs (miRNAs/miR). Moreover, analysis of public data has revealed that miR-4423-3p is significantly downregulated in peripheral blood mononuclear cel...

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Autores principales:	Weihong Xu, Lu Ye, Huaxiang Wu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	rheumatoid arthritis microrna mmp13 fibroblast-like synoviocytes Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/d4763d89a8324655ba80ebdb7c0149e0
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spelling	oai:doaj.org-article:d4763d89a8324655ba80ebdb7c0149e02021-12-01T14:41:00ZMicroRNA-4423-3p inhibits proliferation of fibroblast-like synoviocytes by targeting matrix metalloproteinase 13 in rheumatoid arthritis2165-59792165-598710.1080/21655979.2021.1988372https://doaj.org/article/d4763d89a8324655ba80ebdb7c0149e02021-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1988372https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease that is increasing in incidence worldwide. RA is regulated by a variety of microRNAs (miRNAs/miR). Moreover, analysis of public data has revealed that miR-4423-3p is significantly downregulated in peripheral blood mononuclear cells of RA patients. This study investigated the role of miR-4423-3p in RA. The levels of miR-4423-3p and matrix metalloproteinase 13 (MMP13) in RA patients and the regulatory relationship between miR-4423-3p and MMP13 were analyzed using public data. A dual-luciferase reporter assay was performed to verify that miR-4423-3p targets MMP13 in human fibroblast-like synoviocyte (HFLS) RA cells (HFLS-RA). Following the overexpression of miR-4423-3p, miR-4423-3p inhibitor, and MMP13 in HFLS-RA, viability, proliferation, cell cycle, apoptosis, and invasion/migration assays were used to detect the effects of miR-4423-3p targeting MMP13 on cell biological processes. The results revealed that miR-4423-3p was downregulated in peripheral blood mononuclear cells of RA patients and MMP13 was upregulated in synovial tissue of RA patients. miR-4423-3p targets the 3ʹ untranslated region of MMP13 and downregulates MMP13 expression. After overexpression of miR-4423-3p, cell proliferation, migration, and invasion were inhibited, the cell cycle was prevented and cell apoptosis was promoted. Overexpression of MMP13 promoted cell proliferation, migration, and invasion, while accelerating the cell cycle process and suppressing apoptosis. The findings indicate that in HFLS-RA cells, overexpression of miR-4423-3p inhibited proliferation, migration, and invasion, and promoted apoptosis by negatively regulating MMP13. The overexpression of miR-4423-3p might be a novel strategy for the treatment of RA.Weihong XuLu YeHuaxiang WuTaylor & Francis Grouparticlerheumatoid arthritismicrornammp13fibroblast-like synoviocytesBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 2, Pp 9411-9423 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rheumatoid arthritis microrna mmp13 fibroblast-like synoviocytes Biotechnology TP248.13-248.65
spellingShingle	rheumatoid arthritis microrna mmp13 fibroblast-like synoviocytes Biotechnology TP248.13-248.65 Weihong Xu Lu Ye Huaxiang Wu MicroRNA-4423-3p inhibits proliferation of fibroblast-like synoviocytes by targeting matrix metalloproteinase 13 in rheumatoid arthritis
description	Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease that is increasing in incidence worldwide. RA is regulated by a variety of microRNAs (miRNAs/miR). Moreover, analysis of public data has revealed that miR-4423-3p is significantly downregulated in peripheral blood mononuclear cells of RA patients. This study investigated the role of miR-4423-3p in RA. The levels of miR-4423-3p and matrix metalloproteinase 13 (MMP13) in RA patients and the regulatory relationship between miR-4423-3p and MMP13 were analyzed using public data. A dual-luciferase reporter assay was performed to verify that miR-4423-3p targets MMP13 in human fibroblast-like synoviocyte (HFLS) RA cells (HFLS-RA). Following the overexpression of miR-4423-3p, miR-4423-3p inhibitor, and MMP13 in HFLS-RA, viability, proliferation, cell cycle, apoptosis, and invasion/migration assays were used to detect the effects of miR-4423-3p targeting MMP13 on cell biological processes. The results revealed that miR-4423-3p was downregulated in peripheral blood mononuclear cells of RA patients and MMP13 was upregulated in synovial tissue of RA patients. miR-4423-3p targets the 3ʹ untranslated region of MMP13 and downregulates MMP13 expression. After overexpression of miR-4423-3p, cell proliferation, migration, and invasion were inhibited, the cell cycle was prevented and cell apoptosis was promoted. Overexpression of MMP13 promoted cell proliferation, migration, and invasion, while accelerating the cell cycle process and suppressing apoptosis. The findings indicate that in HFLS-RA cells, overexpression of miR-4423-3p inhibited proliferation, migration, and invasion, and promoted apoptosis by negatively regulating MMP13. The overexpression of miR-4423-3p might be a novel strategy for the treatment of RA.
format	article
author	Weihong Xu Lu Ye Huaxiang Wu
author_facet	Weihong Xu Lu Ye Huaxiang Wu
author_sort	Weihong Xu
title	MicroRNA-4423-3p inhibits proliferation of fibroblast-like synoviocytes by targeting matrix metalloproteinase 13 in rheumatoid arthritis
title_short	MicroRNA-4423-3p inhibits proliferation of fibroblast-like synoviocytes by targeting matrix metalloproteinase 13 in rheumatoid arthritis
title_full	MicroRNA-4423-3p inhibits proliferation of fibroblast-like synoviocytes by targeting matrix metalloproteinase 13 in rheumatoid arthritis
title_fullStr	MicroRNA-4423-3p inhibits proliferation of fibroblast-like synoviocytes by targeting matrix metalloproteinase 13 in rheumatoid arthritis
title_full_unstemmed	MicroRNA-4423-3p inhibits proliferation of fibroblast-like synoviocytes by targeting matrix metalloproteinase 13 in rheumatoid arthritis
title_sort	microrna-4423-3p inhibits proliferation of fibroblast-like synoviocytes by targeting matrix metalloproteinase 13 in rheumatoid arthritis
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/d4763d89a8324655ba80ebdb7c0149e0
work_keys_str_mv	AT weihongxu microrna44233pinhibitsproliferationoffibroblastlikesynoviocytesbytargetingmatrixmetalloproteinase13inrheumatoidarthritis AT luye microrna44233pinhibitsproliferationoffibroblastlikesynoviocytesbytargetingmatrixmetalloproteinase13inrheumatoidarthritis AT huaxiangwu microrna44233pinhibitsproliferationoffibroblastlikesynoviocytesbytargetingmatrixmetalloproteinase13inrheumatoidarthritis
_version_	1718405023589728256

MicroRNA-4423-3p inhibits proliferation of fibroblast-like synoviocytes by targeting matrix metalloproteinase 13 in rheumatoid arthritis

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