Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats

Dzhuliia Sh Dzhalilova,1 Anna M Kosyreva,1 Mikhail E Diatroptov,1 Elena A Ponomarenko,1 Ivan S Tsvetkov,1 Natalia A Zolotova,1 Vladimir A Mkhitarov,2 Dmitry N Khochanskiy,1 Olga V Makarova1 1Department of Immunomorphology of Inflammation, Federal State Budgetary Institutio...

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Autores principales:	Dzhalilova DS, Kosyreva AM, Diatroptov ME, Ponomarenko EA, Tsvetkov IS, Zolotova NA, Mkhitarov VA, Khochanskiy DN, Makarova OV
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	hypoxia inflammation SIRS lipopolysaccharide Hif-1α Nf-kb Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/d484b2a0868a455582392507129043ba
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spelling	oai:doaj.org-article:d484b2a0868a455582392507129043ba2021-12-02T04:12:28ZDependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats1178-7031https://doaj.org/article/d484b2a0868a455582392507129043ba2019-03-01T00:00:00Zhttps://www.dovepress.com/dependence-of-the-severity-of-the-systemic-inflammatory-response-on-re-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Dzhuliia Sh Dzhalilova,1 Anna M Kosyreva,1 Mikhail E Diatroptov,1 Elena A Ponomarenko,1 Ivan S Tsvetkov,1 Natalia A Zolotova,1 Vladimir A Mkhitarov,2 Dmitry N Khochanskiy,1 Olga V Makarova1 1Department of Immunomorphology of Inflammation, Federal State Budgetary Institution “Science Research Institute of Human Morphology”, Moscow, Russia; 2Department of Informatics and Morphometry, Federal State Budgetary Institution “Science Research Institute of Human Morphology”, Moscow, Russia Purpose: The aim of the study was to characterize the severity of the systemic inflammatory response induced by lipopolysaccharide (LPS) in animals with different resistance levels to hypoxia. Materials and methods: Two to three months old male Wistar rats (220–240 g) were divided according to hypoxia tolerance in a hypobaric chamber. After a month, they were injected intraperitoneally with Escherichia coli LPS at a dose of 1.5 mg/kg. After 3, 6 and 24 hours of LPS injection, we studied the levels of IL-1β, C-reactive protein (CRP) and TGF-β in the serum, the expression of Hif-1α and Nf-kb in the liver, morphological disorders in the lung and ex vivo production of IL-10 by splenic cells activated by ConA. Results: In the early periods after the injection of LPS, increase in Nf-kb expression in the liver was observed only in the rats susceptible to hypoxia. After 6 hours of LPS injection, the number of neutrophils in the interalveolar septa of the lungs of rats susceptible to hypoxia was higher than in tolerant rats. This points to the development of more pronounced LPS-induced inflammation in the rats susceptible to hypoxia and is accompanied by increased expression of Hif-1α in the liver after 6 hours of LPS administration, serum IL-1β level after 3 hours and CRP level after 24 hours. The production of the anti-inflammatory cytokine IL-10 by the spleen was significantly decreased after 6 hours of LPS injection only in the animals tolerant to hypoxia. After 24 hours of LPS injection, a significant decrease in serum TGF-β level occurred in the rats tolerant to hypoxia in comparison with the control group, which improved the survival rates of the animals. Conclusion: We have demonstrated the differences in the severity of the LPS-induced inflammatory response in male Wistar rats with different resistance levels to hypoxia. Rats susceptible to hypoxia are characterized by a more pronounced inflammatory response induced by LPS. Keywords: tolerant, susceptible, hypoxia, inflammation, SIRS, lipopolysaccharide, Hif-1α, Nf-kbDzhalilova DSKosyreva AMDiatroptov MEPonomarenko EATsvetkov ISZolotova NAMkhitarov VAKhochanskiy DNMakarova OVDove Medical PressarticlehypoxiainflammationSIRSlipopolysaccharideHif-1αNf-kbPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 12, Pp 73-86 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	hypoxia inflammation SIRS lipopolysaccharide Hif-1α Nf-kb Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	hypoxia inflammation SIRS lipopolysaccharide Hif-1α Nf-kb Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Dzhalilova DS Kosyreva AM Diatroptov ME Ponomarenko EA Tsvetkov IS Zolotova NA Mkhitarov VA Khochanskiy DN Makarova OV Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats
description	Dzhuliia Sh Dzhalilova,1 Anna M Kosyreva,1 Mikhail E Diatroptov,1 Elena A Ponomarenko,1 Ivan S Tsvetkov,1 Natalia A Zolotova,1 Vladimir A Mkhitarov,2 Dmitry N Khochanskiy,1 Olga V Makarova1 1Department of Immunomorphology of Inflammation, Federal State Budgetary Institution “Science Research Institute of Human Morphology”, Moscow, Russia; 2Department of Informatics and Morphometry, Federal State Budgetary Institution “Science Research Institute of Human Morphology”, Moscow, Russia Purpose: The aim of the study was to characterize the severity of the systemic inflammatory response induced by lipopolysaccharide (LPS) in animals with different resistance levels to hypoxia. Materials and methods: Two to three months old male Wistar rats (220–240 g) were divided according to hypoxia tolerance in a hypobaric chamber. After a month, they were injected intraperitoneally with Escherichia coli LPS at a dose of 1.5 mg/kg. After 3, 6 and 24 hours of LPS injection, we studied the levels of IL-1β, C-reactive protein (CRP) and TGF-β in the serum, the expression of Hif-1α and Nf-kb in the liver, morphological disorders in the lung and ex vivo production of IL-10 by splenic cells activated by ConA. Results: In the early periods after the injection of LPS, increase in Nf-kb expression in the liver was observed only in the rats susceptible to hypoxia. After 6 hours of LPS injection, the number of neutrophils in the interalveolar septa of the lungs of rats susceptible to hypoxia was higher than in tolerant rats. This points to the development of more pronounced LPS-induced inflammation in the rats susceptible to hypoxia and is accompanied by increased expression of Hif-1α in the liver after 6 hours of LPS administration, serum IL-1β level after 3 hours and CRP level after 24 hours. The production of the anti-inflammatory cytokine IL-10 by the spleen was significantly decreased after 6 hours of LPS injection only in the animals tolerant to hypoxia. After 24 hours of LPS injection, a significant decrease in serum TGF-β level occurred in the rats tolerant to hypoxia in comparison with the control group, which improved the survival rates of the animals. Conclusion: We have demonstrated the differences in the severity of the LPS-induced inflammatory response in male Wistar rats with different resistance levels to hypoxia. Rats susceptible to hypoxia are characterized by a more pronounced inflammatory response induced by LPS. Keywords: tolerant, susceptible, hypoxia, inflammation, SIRS, lipopolysaccharide, Hif-1α, Nf-kb
format	article
author	Dzhalilova DS Kosyreva AM Diatroptov ME Ponomarenko EA Tsvetkov IS Zolotova NA Mkhitarov VA Khochanskiy DN Makarova OV
author_facet	Dzhalilova DS Kosyreva AM Diatroptov ME Ponomarenko EA Tsvetkov IS Zolotova NA Mkhitarov VA Khochanskiy DN Makarova OV
author_sort	Dzhalilova DS
title	Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats
title_short	Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats
title_full	Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats
title_fullStr	Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats
title_full_unstemmed	Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats
title_sort	dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male wistar rats
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/d484b2a0868a455582392507129043ba
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Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats

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