A Mur regulator protein in the extremophilic bacterium Deinococcus radiodurans.

Ferric uptake regulator (Fur) is a transcriptional regulator that controls the expression of genes involved in the uptake of iron and manganese, as well as vital nutrients, and is essential for intracellular redox cycling. We identified a unique Fur homolog (DR0865) from Deinococcus radiodurans, whi...

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Autores principales:	Amir Miraj Ul Hussain Shah, Ye Zhao, Yunfei Wang, Guoquan Yan, Qikun Zhang, Liangyan Wang, Bing Tian, Huan Chen, Yuejin Hua
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d487064a2fdd42c8ac57bac1ece1c956
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spelling	oai:doaj.org-article:d487064a2fdd42c8ac57bac1ece1c9562021-11-25T05:59:52ZA Mur regulator protein in the extremophilic bacterium Deinococcus radiodurans.1932-620310.1371/journal.pone.0106341https://doaj.org/article/d487064a2fdd42c8ac57bac1ece1c9562014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0106341https://doaj.org/toc/1932-6203Ferric uptake regulator (Fur) is a transcriptional regulator that controls the expression of genes involved in the uptake of iron and manganese, as well as vital nutrients, and is essential for intracellular redox cycling. We identified a unique Fur homolog (DR0865) from Deinococcus radiodurans, which is known for its extreme resistance to radiation and oxidants. A dr0865 mutant (Mt-0865) showed a higher sensitivity to manganese stress, hydrogen peroxide, gamma irradiation and ultraviolet (UV) irradiation than the wild-type R1 strain. Cellular manganese (Mn) ion (Mn2+) analysis showed that Mn2+, copper (Cu2+), and ferric (Fe3+) ions accumulated significantly in the mutant, which suggests that the dr0865 gene is not only involved in the regulation of Mn2+ homeostasis, but also affects the uptake of other ions. In addition, transcriptome profiles under MnCl2 stress showed that the expression of many genes involved in Mn metabolism was significantly different in the wild-type R1 and DR0865 mutant (Mt-0865). Furthermore, we found that the dr0865 gene serves as a positive regulator of the manganese efflux pump gene mntE (dr1236), and as a negative regulator of Mn ABC transporter genes, such as dr2283, dr2284 and dr2523. Therefore, it plays an important role in maintaining the homoeostasis of intracellular Mn (II), and also other Mn2+, zinc (Zn2+) and Cu2+ ions. Based on its role in manganese homeostasis, DR0865 likely belongs to the Mur sub-family of Fur homolog.Amir Miraj Ul Hussain ShahYe ZhaoYunfei WangGuoquan YanQikun ZhangLiangyan WangBing TianHuan ChenYuejin HuaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e106341 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amir Miraj Ul Hussain Shah Ye Zhao Yunfei Wang Guoquan Yan Qikun Zhang Liangyan Wang Bing Tian Huan Chen Yuejin Hua A Mur regulator protein in the extremophilic bacterium Deinococcus radiodurans.
description	Ferric uptake regulator (Fur) is a transcriptional regulator that controls the expression of genes involved in the uptake of iron and manganese, as well as vital nutrients, and is essential for intracellular redox cycling. We identified a unique Fur homolog (DR0865) from Deinococcus radiodurans, which is known for its extreme resistance to radiation and oxidants. A dr0865 mutant (Mt-0865) showed a higher sensitivity to manganese stress, hydrogen peroxide, gamma irradiation and ultraviolet (UV) irradiation than the wild-type R1 strain. Cellular manganese (Mn) ion (Mn2+) analysis showed that Mn2+, copper (Cu2+), and ferric (Fe3+) ions accumulated significantly in the mutant, which suggests that the dr0865 gene is not only involved in the regulation of Mn2+ homeostasis, but also affects the uptake of other ions. In addition, transcriptome profiles under MnCl2 stress showed that the expression of many genes involved in Mn metabolism was significantly different in the wild-type R1 and DR0865 mutant (Mt-0865). Furthermore, we found that the dr0865 gene serves as a positive regulator of the manganese efflux pump gene mntE (dr1236), and as a negative regulator of Mn ABC transporter genes, such as dr2283, dr2284 and dr2523. Therefore, it plays an important role in maintaining the homoeostasis of intracellular Mn (II), and also other Mn2+, zinc (Zn2+) and Cu2+ ions. Based on its role in manganese homeostasis, DR0865 likely belongs to the Mur sub-family of Fur homolog.
format	article
author	Amir Miraj Ul Hussain Shah Ye Zhao Yunfei Wang Guoquan Yan Qikun Zhang Liangyan Wang Bing Tian Huan Chen Yuejin Hua
author_facet	Amir Miraj Ul Hussain Shah Ye Zhao Yunfei Wang Guoquan Yan Qikun Zhang Liangyan Wang Bing Tian Huan Chen Yuejin Hua
author_sort	Amir Miraj Ul Hussain Shah
title	A Mur regulator protein in the extremophilic bacterium Deinococcus radiodurans.
title_short	A Mur regulator protein in the extremophilic bacterium Deinococcus radiodurans.
title_full	A Mur regulator protein in the extremophilic bacterium Deinococcus radiodurans.
title_fullStr	A Mur regulator protein in the extremophilic bacterium Deinococcus radiodurans.
title_full_unstemmed	A Mur regulator protein in the extremophilic bacterium Deinococcus radiodurans.
title_sort	mur regulator protein in the extremophilic bacterium deinococcus radiodurans.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d487064a2fdd42c8ac57bac1ece1c956
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A Mur regulator protein in the extremophilic bacterium Deinococcus radiodurans.

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