VIPER regulates naive T cell activation and effector responses: Implication in TLR4 associated acute stage T cell responses

Abstract Naive T cells are known to express the modest level of TLR4 while it is known to go down during TCR activation. However, information towards the requirement of TLR4 signaling during TCR or mitogenic activation of naive wild-type T cells remains scanty. Here we have investigated the endogeno...

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Autores principales:	Subhransu Sekhar Sahoo, Belluru M. Pratheek, Vikram S. Meena, Tapas Kumar Nayak, P. Sanjai Kumar, Saumya Bandyopadhyay, Prasanta Kumar Maiti, Subhasis Chattopadhyay
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d48983d540ff4678be75534926fc1dc9
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spelling	oai:doaj.org-article:d48983d540ff4678be75534926fc1dc92021-12-02T16:08:25ZVIPER regulates naive T cell activation and effector responses: Implication in TLR4 associated acute stage T cell responses10.1038/s41598-018-25549-82045-2322https://doaj.org/article/d48983d540ff4678be75534926fc1dc92018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25549-8https://doaj.org/toc/2045-2322Abstract Naive T cells are known to express the modest level of TLR4 while it is known to go down during TCR activation. However, information towards the requirement of TLR4 signaling during TCR or mitogenic activation of naive wild-type T cells remains scanty. Here we have investigated the endogenous functional expression of TLR4 in naive mice T cells during TCR and mitogenic stimulation in presence of VIPER peptide (VP), an established inhibitor of TLR4 signaling. As expected we found that TLR4 expression goes down during TCR and mitogenic activation. Interestingly, we observed that VP treatment restores TLR4 expression on those activated T cells. Moreover, VP was found to regulate such activation of naive T cell as evident by reduction of CD25, CD69 expression, effector cytokines (IL-2, IFN-γ, TNF) production, T cell proliferation and down-regulation of T cell activation-dependent Fas (CD95), FasL (CD95L) expression. Together, our current observation highlights a possible requirement of TLR4 responses in T cells, which might have possible implication towards the pathogenic acute phase activation of naive T cells.Subhransu Sekhar SahooBelluru M. PratheekVikram S. MeenaTapas Kumar NayakP. Sanjai KumarSaumya BandyopadhyayPrasanta Kumar MaitiSubhasis ChattopadhyayNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Subhransu Sekhar Sahoo Belluru M. Pratheek Vikram S. Meena Tapas Kumar Nayak P. Sanjai Kumar Saumya Bandyopadhyay Prasanta Kumar Maiti Subhasis Chattopadhyay VIPER regulates naive T cell activation and effector responses: Implication in TLR4 associated acute stage T cell responses
description	Abstract Naive T cells are known to express the modest level of TLR4 while it is known to go down during TCR activation. However, information towards the requirement of TLR4 signaling during TCR or mitogenic activation of naive wild-type T cells remains scanty. Here we have investigated the endogenous functional expression of TLR4 in naive mice T cells during TCR and mitogenic stimulation in presence of VIPER peptide (VP), an established inhibitor of TLR4 signaling. As expected we found that TLR4 expression goes down during TCR and mitogenic activation. Interestingly, we observed that VP treatment restores TLR4 expression on those activated T cells. Moreover, VP was found to regulate such activation of naive T cell as evident by reduction of CD25, CD69 expression, effector cytokines (IL-2, IFN-γ, TNF) production, T cell proliferation and down-regulation of T cell activation-dependent Fas (CD95), FasL (CD95L) expression. Together, our current observation highlights a possible requirement of TLR4 responses in T cells, which might have possible implication towards the pathogenic acute phase activation of naive T cells.
format	article
author	Subhransu Sekhar Sahoo Belluru M. Pratheek Vikram S. Meena Tapas Kumar Nayak P. Sanjai Kumar Saumya Bandyopadhyay Prasanta Kumar Maiti Subhasis Chattopadhyay
author_facet	Subhransu Sekhar Sahoo Belluru M. Pratheek Vikram S. Meena Tapas Kumar Nayak P. Sanjai Kumar Saumya Bandyopadhyay Prasanta Kumar Maiti Subhasis Chattopadhyay
author_sort	Subhransu Sekhar Sahoo
title	VIPER regulates naive T cell activation and effector responses: Implication in TLR4 associated acute stage T cell responses
title_short	VIPER regulates naive T cell activation and effector responses: Implication in TLR4 associated acute stage T cell responses
title_full	VIPER regulates naive T cell activation and effector responses: Implication in TLR4 associated acute stage T cell responses
title_fullStr	VIPER regulates naive T cell activation and effector responses: Implication in TLR4 associated acute stage T cell responses
title_full_unstemmed	VIPER regulates naive T cell activation and effector responses: Implication in TLR4 associated acute stage T cell responses
title_sort	viper regulates naive t cell activation and effector responses: implication in tlr4 associated acute stage t cell responses
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/d48983d540ff4678be75534926fc1dc9
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VIPER regulates naive T cell activation and effector responses: Implication in TLR4 associated acute stage T cell responses

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