MALDI-TOF mass spectrometric protein profiling of microvesicles produced by the NK-92 natural killer cell line

Extracellular vesicles that are shed from the plasma membrane contain a wide range of molecules, among which are proteins, lipids, nucleic acids, and sugars. The cytotoxic proteins of natural killer cells play a key role in the implementation of their cytolytic functions. One of the important s...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	A. V. Korenevsky, A. D. Shcherbitskaia, M. E. Berezkina, K. L. Markova, E. P. Alexandrova, O. A. Balabas, S. A. Selkov, D. I. Sokolov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2020
Materias:	natural killer cells microvesicles immune response inflammation proteome maldi-tof mass spectrometry Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/d49151a07c204b98bbe1fbd9579d164f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d49151a07c204b98bbe1fbd9579d164f
record_format	dspace
spelling	oai:doaj.org-article:d49151a07c204b98bbe1fbd9579d164f2021-11-18T08:03:49ZMALDI-TOF mass spectrometric protein profiling of microvesicles produced by the NK-92 natural killer cell line1563-06252313-741X10.15789/1563-0625-MMS-1976https://doaj.org/article/d49151a07c204b98bbe1fbd9579d164f2020-08-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1976https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XExtracellular vesicles that are shed from the plasma membrane contain a wide range of molecules, among which are proteins, lipids, nucleic acids, and sugars. The cytotoxic proteins of natural killer cells play a key role in the implementation of their cytolytic functions. One of the important steps in understanding the distant communication of cells is the determination of the proteome of microvesicles. This study was aimed at the protein profiling of the microvesicles produced by the NK-92 natural killer cell line. 986 proteins with a variety of functions were identified in the lysate of microvesicles using the MALDI-TOF mass spectrometric analysis. With automated methods of functional analysis applied, it has been shown that the largest protein groups are hypothetical proteins, proteins with unknown functions, and domains. The most representative groups are also comprised by transcription regulators; intracellular signaling proteins; RNA translation, transcription, processing, and utilization regulators; receptors; protein processing and proteolysis regulators; amino acid metabolism enzymes, as well as transport proteins and transport regulators. Minor functional groups are represented by vitamins and mineral metabolism enzymes, membrane and microdomain-forming proteins, hormones, hemostatic regulators, regulators of sensory systems, specific mitochondrial and Golgi apparatus proteins, and extracellular signaling proteins. An intermediate position is occupied by various functional groups, including cytoskeleton and motor proteins; proteins of centrioles; ion channels and their regulators; proteins of the ubiquitin-proteasome pathway of protein degradation; lipid, steroid, and fatty acid metabolism enzymes; nucleic acid base and carbohydrate metabolism enzymes, as well as energy metabolism enzymes and other proteins involved in intermediate metabolism; proteins of the immune response and inflammation; antigens and histocompatibility proteins; cytokines and growth factors; regulators of apoptosis, autophagy, endocytosis, and exocytosis; regulators of the cell cycle and division; regulators of proliferation, cell differentiation, and morphogenesis; regulators of cell adhesion and matrix metabolism; nuclear transport proteins; transposition proteins; DNA replication and repair proteins, as well as inactive proteins. The data obtained expand the existing knowledge of the distant communication of cells and indicate new mechanisms of interaction between natural killer and target cells.A. V. KorenevskyA. D. ShcherbitskaiaM. E. BerezkinaK. L. MarkovaE. P. AlexandrovaO. A. BalabasS. A. SelkovD. I. SokolovSPb RAACIarticlenatural killer cellsmicrovesiclesimmune responseinflammationproteomemaldi-tof mass spectrometryImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 22, Iss 4, Pp 633-646 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	natural killer cells microvesicles immune response inflammation proteome maldi-tof mass spectrometry Immunologic diseases. Allergy RC581-607
spellingShingle	natural killer cells microvesicles immune response inflammation proteome maldi-tof mass spectrometry Immunologic diseases. Allergy RC581-607 A. V. Korenevsky A. D. Shcherbitskaia M. E. Berezkina K. L. Markova E. P. Alexandrova O. A. Balabas S. A. Selkov D. I. Sokolov MALDI-TOF mass spectrometric protein profiling of microvesicles produced by the NK-92 natural killer cell line
description	Extracellular vesicles that are shed from the plasma membrane contain a wide range of molecules, among which are proteins, lipids, nucleic acids, and sugars. The cytotoxic proteins of natural killer cells play a key role in the implementation of their cytolytic functions. One of the important steps in understanding the distant communication of cells is the determination of the proteome of microvesicles. This study was aimed at the protein profiling of the microvesicles produced by the NK-92 natural killer cell line. 986 proteins with a variety of functions were identified in the lysate of microvesicles using the MALDI-TOF mass spectrometric analysis. With automated methods of functional analysis applied, it has been shown that the largest protein groups are hypothetical proteins, proteins with unknown functions, and domains. The most representative groups are also comprised by transcription regulators; intracellular signaling proteins; RNA translation, transcription, processing, and utilization regulators; receptors; protein processing and proteolysis regulators; amino acid metabolism enzymes, as well as transport proteins and transport regulators. Minor functional groups are represented by vitamins and mineral metabolism enzymes, membrane and microdomain-forming proteins, hormones, hemostatic regulators, regulators of sensory systems, specific mitochondrial and Golgi apparatus proteins, and extracellular signaling proteins. An intermediate position is occupied by various functional groups, including cytoskeleton and motor proteins; proteins of centrioles; ion channels and their regulators; proteins of the ubiquitin-proteasome pathway of protein degradation; lipid, steroid, and fatty acid metabolism enzymes; nucleic acid base and carbohydrate metabolism enzymes, as well as energy metabolism enzymes and other proteins involved in intermediate metabolism; proteins of the immune response and inflammation; antigens and histocompatibility proteins; cytokines and growth factors; regulators of apoptosis, autophagy, endocytosis, and exocytosis; regulators of the cell cycle and division; regulators of proliferation, cell differentiation, and morphogenesis; regulators of cell adhesion and matrix metabolism; nuclear transport proteins; transposition proteins; DNA replication and repair proteins, as well as inactive proteins. The data obtained expand the existing knowledge of the distant communication of cells and indicate new mechanisms of interaction between natural killer and target cells.
format	article
author	A. V. Korenevsky A. D. Shcherbitskaia M. E. Berezkina K. L. Markova E. P. Alexandrova O. A. Balabas S. A. Selkov D. I. Sokolov
author_facet	A. V. Korenevsky A. D. Shcherbitskaia M. E. Berezkina K. L. Markova E. P. Alexandrova O. A. Balabas S. A. Selkov D. I. Sokolov
author_sort	A. V. Korenevsky
title	MALDI-TOF mass spectrometric protein profiling of microvesicles produced by the NK-92 natural killer cell line
title_short	MALDI-TOF mass spectrometric protein profiling of microvesicles produced by the NK-92 natural killer cell line
title_full	MALDI-TOF mass spectrometric protein profiling of microvesicles produced by the NK-92 natural killer cell line
title_fullStr	MALDI-TOF mass spectrometric protein profiling of microvesicles produced by the NK-92 natural killer cell line
title_full_unstemmed	MALDI-TOF mass spectrometric protein profiling of microvesicles produced by the NK-92 natural killer cell line
title_sort	maldi-tof mass spectrometric protein profiling of microvesicles produced by the nk-92 natural killer cell line
publisher	SPb RAACI
publishDate	2020
url	https://doaj.org/article/d49151a07c204b98bbe1fbd9579d164f
work_keys_str_mv	AT avkorenevsky malditofmassspectrometricproteinprofilingofmicrovesiclesproducedbythenk92naturalkillercellline AT adshcherbitskaia malditofmassspectrometricproteinprofilingofmicrovesiclesproducedbythenk92naturalkillercellline AT meberezkina malditofmassspectrometricproteinprofilingofmicrovesiclesproducedbythenk92naturalkillercellline AT klmarkova malditofmassspectrometricproteinprofilingofmicrovesiclesproducedbythenk92naturalkillercellline AT epalexandrova malditofmassspectrometricproteinprofilingofmicrovesiclesproducedbythenk92naturalkillercellline AT oabalabas malditofmassspectrometricproteinprofilingofmicrovesiclesproducedbythenk92naturalkillercellline AT saselkov malditofmassspectrometricproteinprofilingofmicrovesiclesproducedbythenk92naturalkillercellline AT disokolov malditofmassspectrometricproteinprofilingofmicrovesiclesproducedbythenk92naturalkillercellline
_version_	1718422318927052800

MALDI-TOF mass spectrometric protein profiling of microvesicles produced by the NK-92 natural killer cell line

Ejemplares similares