Quantitative FRET (qFRET) Technology for the Determination of Protein–Protein Interaction Affinity in Solution

Protein–protein interactions play pivotal roles in life, and the protein interaction affinity confers specific protein interaction events in physiology or pathology. Förster resonance energy transfer (FRET) has been widely used in biological and biomedical research to detect molecular interactions i...

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Autores principales:	Jiayu Liao, Vipul Madahar, Runrui Dang, Ling Jiang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	protein interaction affinity quantitative FRET assay <i>K<sub>D</sub></i> determination FRET excitation FRET quenching Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/d4917dd22de74b3ca1b7c224c6857bab
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spelling	oai:doaj.org-article:d4917dd22de74b3ca1b7c224c6857bab2021-11-11T18:22:20ZQuantitative FRET (qFRET) Technology for the Determination of Protein–Protein Interaction Affinity in Solution10.3390/molecules262163391420-3049https://doaj.org/article/d4917dd22de74b3ca1b7c224c6857bab2021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6339https://doaj.org/toc/1420-3049Protein–protein interactions play pivotal roles in life, and the protein interaction affinity confers specific protein interaction events in physiology or pathology. Förster resonance energy transfer (FRET) has been widely used in biological and biomedical research to detect molecular interactions in vitro and in vivo. The FRET assay provides very high sensitivity and efficiency. Several attempts have been made to develop the FRET assay into a quantitative measurement for protein–protein interaction affinity in the past. However, the progress has been slow due to complicated procedures or because of challenges in differentiating the FRET signal from other direct emission signals from donor and receptor. This review focuses on recent developments of the quantitative FRET analysis and its application in the determination of protein–protein interaction affinity (<i>K<sub>D</sub></i>), either through FRET acceptor emission or donor quenching methods. This paper mainly reviews novel theatrical developments and experimental procedures rather than specific experimental results. The FRET-based approach for protein interaction affinity determination provides several advantages, including high sensitivity, high accuracy, low cost, and high-throughput assay. The FRET-based methodology holds excellent potential for those difficult-to-be expressed proteins and for protein interactions in living cells.Jiayu LiaoVipul MadaharRunrui DangLing JiangMDPI AGarticleprotein interaction affinityquantitative FRET assay<i>K<sub>D</sub></i> determinationFRET excitationFRET quenchingOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6339, p 6339 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	protein interaction affinity quantitative FRET assay <i>K<sub>D</sub></i> determination FRET excitation FRET quenching Organic chemistry QD241-441
spellingShingle	protein interaction affinity quantitative FRET assay <i>K<sub>D</sub></i> determination FRET excitation FRET quenching Organic chemistry QD241-441 Jiayu Liao Vipul Madahar Runrui Dang Ling Jiang Quantitative FRET (qFRET) Technology for the Determination of Protein–Protein Interaction Affinity in Solution
description	Protein–protein interactions play pivotal roles in life, and the protein interaction affinity confers specific protein interaction events in physiology or pathology. Förster resonance energy transfer (FRET) has been widely used in biological and biomedical research to detect molecular interactions in vitro and in vivo. The FRET assay provides very high sensitivity and efficiency. Several attempts have been made to develop the FRET assay into a quantitative measurement for protein–protein interaction affinity in the past. However, the progress has been slow due to complicated procedures or because of challenges in differentiating the FRET signal from other direct emission signals from donor and receptor. This review focuses on recent developments of the quantitative FRET analysis and its application in the determination of protein–protein interaction affinity (<i>K<sub>D</sub></i>), either through FRET acceptor emission or donor quenching methods. This paper mainly reviews novel theatrical developments and experimental procedures rather than specific experimental results. The FRET-based approach for protein interaction affinity determination provides several advantages, including high sensitivity, high accuracy, low cost, and high-throughput assay. The FRET-based methodology holds excellent potential for those difficult-to-be expressed proteins and for protein interactions in living cells.
format	article
author	Jiayu Liao Vipul Madahar Runrui Dang Ling Jiang
author_facet	Jiayu Liao Vipul Madahar Runrui Dang Ling Jiang
author_sort	Jiayu Liao
title	Quantitative FRET (qFRET) Technology for the Determination of Protein–Protein Interaction Affinity in Solution
title_short	Quantitative FRET (qFRET) Technology for the Determination of Protein–Protein Interaction Affinity in Solution
title_full	Quantitative FRET (qFRET) Technology for the Determination of Protein–Protein Interaction Affinity in Solution
title_fullStr	Quantitative FRET (qFRET) Technology for the Determination of Protein–Protein Interaction Affinity in Solution
title_full_unstemmed	Quantitative FRET (qFRET) Technology for the Determination of Protein–Protein Interaction Affinity in Solution
title_sort	quantitative fret (qfret) technology for the determination of protein–protein interaction affinity in solution
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d4917dd22de74b3ca1b7c224c6857bab
work_keys_str_mv	AT jiayuliao quantitativefretqfrettechnologyforthedeterminationofproteinproteininteractionaffinityinsolution AT vipulmadahar quantitativefretqfrettechnologyforthedeterminationofproteinproteininteractionaffinityinsolution AT runruidang quantitativefretqfrettechnologyforthedeterminationofproteinproteininteractionaffinityinsolution AT lingjiang quantitativefretqfrettechnologyforthedeterminationofproteinproteininteractionaffinityinsolution
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Quantitative FRET (qFRET) Technology for the Determination of Protein–Protein Interaction Affinity in Solution

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