Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for CO2 valorization

Abstract Background CO2 valorization is one of the effective methods to solve current environmental and energy problems, in which microbial electrosynthesis (MES) system has proved feasible and efficient. Cupriviadus necator (Ralstonia eutropha) H16, a model chemolithoautotroph, is a microbe of choi...

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Autores principales:	Haojie Pan, Jia Wang, Haoliang Wu, Zhongjian Li, Jiazhang Lian
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Cupriviadus necator H16 Ralstonia eutropha H16 Synthetic biology Metabolic engineering CO2 conversion Biomanufacturing Fuel TP315-360 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/d49def3b0351472a95fe97e188cefde5
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spelling	oai:doaj.org-article:d49def3b0351472a95fe97e188cefde52021-11-08T10:56:39ZSynthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for CO2 valorization10.1186/s13068-021-02063-01754-6834https://doaj.org/article/d49def3b0351472a95fe97e188cefde52021-11-01T00:00:00Zhttps://doi.org/10.1186/s13068-021-02063-0https://doaj.org/toc/1754-6834Abstract Background CO2 valorization is one of the effective methods to solve current environmental and energy problems, in which microbial electrosynthesis (MES) system has proved feasible and efficient. Cupriviadus necator (Ralstonia eutropha) H16, a model chemolithoautotroph, is a microbe of choice for CO2 conversion, especially with the ability to be employed in MES due to the presence of genes encoding [NiFe]-hydrogenases and all the Calvin–Benson–Basham cycle enzymes. The CO2 valorization strategy will make sense because the required hydrogen can be produced from renewable electricity independently of fossil fuels. Main body In this review, synthetic biology toolkit for C. necator H16, including genetic engineering vectors, heterologous gene expression elements, platform strain and genome engineering, and transformation strategies, is firstly summarized. Then, the review discusses how to apply these tools to make C. necator H16 an efficient cell factory for converting CO2 to value-added products, with the examples of alcohols, fatty acids, and terpenoids. The review is concluded with the limitation of current genetic tools and perspectives on the development of more efficient and convenient methods as well as the extensive applications of C. necator H16. Conclusions Great progress has been made on genetic engineering toolkit and synthetic biology applications of C. necator H16. Nevertheless, more efforts are expected in the near future to engineer C. necator H16 as efficient cell factories for the conversion of CO2 to value-added products.Haojie PanJia WangHaoliang WuZhongjian LiJiazhang LianBMCarticleCupriviadus necator H16Ralstonia eutropha H16Synthetic biologyMetabolic engineeringCO2 conversionBiomanufacturingFuelTP315-360BiotechnologyTP248.13-248.65ENBiotechnology for Biofuels, Vol 14, Iss 1, Pp 1-20 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cupriviadus necator H16 Ralstonia eutropha H16 Synthetic biology Metabolic engineering CO2 conversion Biomanufacturing Fuel TP315-360 Biotechnology TP248.13-248.65
spellingShingle	Cupriviadus necator H16 Ralstonia eutropha H16 Synthetic biology Metabolic engineering CO2 conversion Biomanufacturing Fuel TP315-360 Biotechnology TP248.13-248.65 Haojie Pan Jia Wang Haoliang Wu Zhongjian Li Jiazhang Lian Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for CO2 valorization
description	Abstract Background CO2 valorization is one of the effective methods to solve current environmental and energy problems, in which microbial electrosynthesis (MES) system has proved feasible and efficient. Cupriviadus necator (Ralstonia eutropha) H16, a model chemolithoautotroph, is a microbe of choice for CO2 conversion, especially with the ability to be employed in MES due to the presence of genes encoding [NiFe]-hydrogenases and all the Calvin–Benson–Basham cycle enzymes. The CO2 valorization strategy will make sense because the required hydrogen can be produced from renewable electricity independently of fossil fuels. Main body In this review, synthetic biology toolkit for C. necator H16, including genetic engineering vectors, heterologous gene expression elements, platform strain and genome engineering, and transformation strategies, is firstly summarized. Then, the review discusses how to apply these tools to make C. necator H16 an efficient cell factory for converting CO2 to value-added products, with the examples of alcohols, fatty acids, and terpenoids. The review is concluded with the limitation of current genetic tools and perspectives on the development of more efficient and convenient methods as well as the extensive applications of C. necator H16. Conclusions Great progress has been made on genetic engineering toolkit and synthetic biology applications of C. necator H16. Nevertheless, more efforts are expected in the near future to engineer C. necator H16 as efficient cell factories for the conversion of CO2 to value-added products.
format	article
author	Haojie Pan Jia Wang Haoliang Wu Zhongjian Li Jiazhang Lian
author_facet	Haojie Pan Jia Wang Haoliang Wu Zhongjian Li Jiazhang Lian
author_sort	Haojie Pan
title	Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for CO2 valorization
title_short	Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for CO2 valorization
title_full	Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for CO2 valorization
title_fullStr	Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for CO2 valorization
title_full_unstemmed	Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for CO2 valorization
title_sort	synthetic biology toolkit for engineering cupriviadus necator h16 as a platform for co2 valorization
publisher	BMC
publishDate	2021
url	https://doaj.org/article/d49def3b0351472a95fe97e188cefde5
work_keys_str_mv	AT haojiepan syntheticbiologytoolkitforengineeringcupriviadusnecatorh16asaplatformforco2valorization AT jiawang syntheticbiologytoolkitforengineeringcupriviadusnecatorh16asaplatformforco2valorization AT haoliangwu syntheticbiologytoolkitforengineeringcupriviadusnecatorh16asaplatformforco2valorization AT zhongjianli syntheticbiologytoolkitforengineeringcupriviadusnecatorh16asaplatformforco2valorization AT jiazhanglian syntheticbiologytoolkitforengineeringcupriviadusnecatorh16asaplatformforco2valorization
_version_	1718442570942513152

Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for CO2 valorization

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