Identification of unique gene expression profile in children with regressive autism spectrum disorder (ASD) and ileocolitis.

Gastrointestinal symptoms are common in children with autism spectrum disorder (ASD) and are often associated with mucosal inflammatory infiltrates of the small and large intestine. Although distinct histologic and immunohistochemical properties of this inflammatory infiltrate have been previously d...

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Autores principales:	Stephen J Walker, John Fortunato, Lenny G Gonzalez, Arthur Krigsman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d4b15115e5524d0b85d40413fe6bc645
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spelling	oai:doaj.org-article:d4b15115e5524d0b85d40413fe6bc6452021-11-18T07:54:12ZIdentification of unique gene expression profile in children with regressive autism spectrum disorder (ASD) and ileocolitis.1932-620310.1371/journal.pone.0058058https://doaj.org/article/d4b15115e5524d0b85d40413fe6bc6452013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23520485/?tool=EBIhttps://doaj.org/toc/1932-6203Gastrointestinal symptoms are common in children with autism spectrum disorder (ASD) and are often associated with mucosal inflammatory infiltrates of the small and large intestine. Although distinct histologic and immunohistochemical properties of this inflammatory infiltrate have been previously described in this ASD(GI) group, molecular characterization of these lesions has not been reported. In this study we utilize transcriptome profiling of gastrointestinal mucosal biopsy tissue from ASD(GI) children and three non-ASD control groups (Crohn's disease, ulcerative colitis, and histologically normal) in an effort to determine if there is a gene expression profile unique to the ASD(GI) group. Comparison of differentially expressed transcripts between the groups demonstrated that non-pathologic (normal) tissue segregated almost completely from inflamed tissue in all cases. Gene expression profiles in intestinal biopsy tissue from patients with Crohn's disease, ulcerative colitis, and ASD(GI), while having significant overlap with each other, also showed distinctive features for each group. Taken together, these results demonstrate that ASD(GI) children have a gastrointestinal mucosal molecular profile that overlaps significantly with known inflammatory bowel disease (IBD), yet has distinctive features that further supports the presence of an ASD-associated IBD variant, or, alternatively, a prodromal phase of typical inflammatory bowel disease. Although we report qPCR confirmation of representative differentially expressed transcripts determined initially by microarray, these findings may be considered preliminary to the extent that they require further confirmation in a validation cohort.Stephen J WalkerJohn FortunatoLenny G GonzalezArthur KrigsmanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58058 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stephen J Walker John Fortunato Lenny G Gonzalez Arthur Krigsman Identification of unique gene expression profile in children with regressive autism spectrum disorder (ASD) and ileocolitis.
description	Gastrointestinal symptoms are common in children with autism spectrum disorder (ASD) and are often associated with mucosal inflammatory infiltrates of the small and large intestine. Although distinct histologic and immunohistochemical properties of this inflammatory infiltrate have been previously described in this ASD(GI) group, molecular characterization of these lesions has not been reported. In this study we utilize transcriptome profiling of gastrointestinal mucosal biopsy tissue from ASD(GI) children and three non-ASD control groups (Crohn's disease, ulcerative colitis, and histologically normal) in an effort to determine if there is a gene expression profile unique to the ASD(GI) group. Comparison of differentially expressed transcripts between the groups demonstrated that non-pathologic (normal) tissue segregated almost completely from inflamed tissue in all cases. Gene expression profiles in intestinal biopsy tissue from patients with Crohn's disease, ulcerative colitis, and ASD(GI), while having significant overlap with each other, also showed distinctive features for each group. Taken together, these results demonstrate that ASD(GI) children have a gastrointestinal mucosal molecular profile that overlaps significantly with known inflammatory bowel disease (IBD), yet has distinctive features that further supports the presence of an ASD-associated IBD variant, or, alternatively, a prodromal phase of typical inflammatory bowel disease. Although we report qPCR confirmation of representative differentially expressed transcripts determined initially by microarray, these findings may be considered preliminary to the extent that they require further confirmation in a validation cohort.
format	article
author	Stephen J Walker John Fortunato Lenny G Gonzalez Arthur Krigsman
author_facet	Stephen J Walker John Fortunato Lenny G Gonzalez Arthur Krigsman
author_sort	Stephen J Walker
title	Identification of unique gene expression profile in children with regressive autism spectrum disorder (ASD) and ileocolitis.
title_short	Identification of unique gene expression profile in children with regressive autism spectrum disorder (ASD) and ileocolitis.
title_full	Identification of unique gene expression profile in children with regressive autism spectrum disorder (ASD) and ileocolitis.
title_fullStr	Identification of unique gene expression profile in children with regressive autism spectrum disorder (ASD) and ileocolitis.
title_full_unstemmed	Identification of unique gene expression profile in children with regressive autism spectrum disorder (ASD) and ileocolitis.
title_sort	identification of unique gene expression profile in children with regressive autism spectrum disorder (asd) and ileocolitis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d4b15115e5524d0b85d40413fe6bc645
work_keys_str_mv	AT stephenjwalker identificationofuniquegeneexpressionprofileinchildrenwithregressiveautismspectrumdisorderasdandileocolitis AT johnfortunato identificationofuniquegeneexpressionprofileinchildrenwithregressiveautismspectrumdisorderasdandileocolitis AT lennyggonzalez identificationofuniquegeneexpressionprofileinchildrenwithregressiveautismspectrumdisorderasdandileocolitis AT arthurkrigsman identificationofuniquegeneexpressionprofileinchildrenwithregressiveautismspectrumdisorderasdandileocolitis
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Identification of unique gene expression profile in children with regressive autism spectrum disorder (ASD) and ileocolitis.

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