Influence of Ethylene Inhibitors and Ethrel on Production of Protocorm Like Bodies in Orchid-Dendrobium 'Sonia'

To increase the efficiency of production of protocorm like bodies, ethylene inhibitors like silver nitrate, salicylic acid and cobalt chloride at three concentrations, viz., 2, 5 and 20 μM and ethrel at 0.7, 1.4 and 14.0 μM were tested, by supplementing MS basal with BAP 4.44 μM. The explant used wa...

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Autores principales:	G V S Saiprasad, P Raghuveer
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2007
Materias:	somatic embryogenesis methane silver nitrate cobalt chloride salicylic acid Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/d4bb25d67a4b4c0da1f2b8d5ac166dcb
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spelling	oai:doaj.org-article:d4bb25d67a4b4c0da1f2b8d5ac166dcb2021-12-02T12:06:39ZInfluence of Ethylene Inhibitors and Ethrel on Production of Protocorm Like Bodies in Orchid-Dendrobium 'Sonia'0973-354X2582-4899https://doaj.org/article/d4bb25d67a4b4c0da1f2b8d5ac166dcb2007-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/631https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899To increase the efficiency of production of protocorm like bodies, ethylene inhibitors like silver nitrate, salicylic acid and cobalt chloride at three concentrations, viz., 2, 5 and 20 μM and ethrel at 0.7, 1.4 and 14.0 μM were tested, by supplementing MS basal with BAP 4.44 μM. The explant used was fractionated protocorm like bodies (plb). Ethylene and methane gases evolved by the explant in the culture container were measured at 20 and 40 days after inoculation (DAI). Among various ethylene inhibitors tested, the maximum number of plb's were produced in media containing silver nitrate (5 μM), cobalt chloride (2 μM) and salicylic acid (20 μM). All ethrel treatments produced succulent, vitrified and deformed plb's. No ethylene evolution was observed in any of the ethylene inhibitor treatments. Only in ethrel treatments was evolution of ethylene observed. Methane evolution was observed in all the ethylene inhibitor treatments. Absolute amounts of methane evolved could not be related to the observed plb production. However, when the evolution of methane was more than 1 nano mole g-1 FW h-1, poor plb production was observed.G V S SaiprasadP RaghuveerSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlesomatic embryogenesismethanesilver nitratecobalt chloridesalicylic acidPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 2, Iss 1, Pp 13-18 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	somatic embryogenesis methane silver nitrate cobalt chloride salicylic acid Plant culture SB1-1110
spellingShingle	somatic embryogenesis methane silver nitrate cobalt chloride salicylic acid Plant culture SB1-1110 G V S Saiprasad P Raghuveer Influence of Ethylene Inhibitors and Ethrel on Production of Protocorm Like Bodies in Orchid-Dendrobium 'Sonia'
description	To increase the efficiency of production of protocorm like bodies, ethylene inhibitors like silver nitrate, salicylic acid and cobalt chloride at three concentrations, viz., 2, 5 and 20 μM and ethrel at 0.7, 1.4 and 14.0 μM were tested, by supplementing MS basal with BAP 4.44 μM. The explant used was fractionated protocorm like bodies (plb). Ethylene and methane gases evolved by the explant in the culture container were measured at 20 and 40 days after inoculation (DAI). Among various ethylene inhibitors tested, the maximum number of plb's were produced in media containing silver nitrate (5 μM), cobalt chloride (2 μM) and salicylic acid (20 μM). All ethrel treatments produced succulent, vitrified and deformed plb's. No ethylene evolution was observed in any of the ethylene inhibitor treatments. Only in ethrel treatments was evolution of ethylene observed. Methane evolution was observed in all the ethylene inhibitor treatments. Absolute amounts of methane evolved could not be related to the observed plb production. However, when the evolution of methane was more than 1 nano mole g-1 FW h-1, poor plb production was observed.
format	article
author	G V S Saiprasad P Raghuveer
author_facet	G V S Saiprasad P Raghuveer
author_sort	G V S Saiprasad
title	Influence of Ethylene Inhibitors and Ethrel on Production of Protocorm Like Bodies in Orchid-Dendrobium 'Sonia'
title_short	Influence of Ethylene Inhibitors and Ethrel on Production of Protocorm Like Bodies in Orchid-Dendrobium 'Sonia'
title_full	Influence of Ethylene Inhibitors and Ethrel on Production of Protocorm Like Bodies in Orchid-Dendrobium 'Sonia'
title_fullStr	Influence of Ethylene Inhibitors and Ethrel on Production of Protocorm Like Bodies in Orchid-Dendrobium 'Sonia'
title_full_unstemmed	Influence of Ethylene Inhibitors and Ethrel on Production of Protocorm Like Bodies in Orchid-Dendrobium 'Sonia'
title_sort	influence of ethylene inhibitors and ethrel on production of protocorm like bodies in orchid-dendrobium 'sonia'
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2007
url	https://doaj.org/article/d4bb25d67a4b4c0da1f2b8d5ac166dcb
work_keys_str_mv	AT gvssaiprasad influenceofethyleneinhibitorsandethrelonproductionofprotocormlikebodiesinorchiddendrobiumsonia AT praghuveer influenceofethyleneinhibitorsandethrelonproductionofprotocormlikebodiesinorchiddendrobiumsonia
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Influence of Ethylene Inhibitors and Ethrel on Production of Protocorm Like Bodies in Orchid-Dendrobium 'Sonia'

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