The Type III Effectome of the Symbiotic Bradyrhizobium vignae Strain ORS3257

Many Bradyrhizobium strains are able to establish a Nod factor-independent symbiosis with the leguminous plant Aeschynomene indica by the use of a type III secretion system (T3SS). Recently, an important advance in the understanding of the molecular factors supp...

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Autores principales:	Nicolas Busset, Djamel Gully, Albin Teulet, Joël Fardoux, Alicia Camuel, David Cornu, Dany Severac, Eric Giraud, Peter Mergaert
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Bradyrhizobium</i> type III secretion system effector nodulation legume symbiosis nod factor Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d4c2837411724f72a0442f57a4d63c35
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spelling	oai:doaj.org-article:d4c2837411724f72a0442f57a4d63c352021-11-25T16:52:35ZThe Type III Effectome of the Symbiotic <i>Bradyrhizobium</i> <i>vignae</i> Strain ORS325710.3390/biom111115922218-273Xhttps://doaj.org/article/d4c2837411724f72a0442f57a4d63c352021-10-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1592https://doaj.org/toc/2218-273XMany <i>Bradyrhizobium</i> strains are able to establish a Nod factor-independent symbiosis with the leguminous plant <i>Aeschynomene indica</i> by the use of a type III secretion system (T3SS). Recently, an important advance in the understanding of the molecular factors supporting this symbiosis has been achieved by the in silico identification and functional characterization of 27 putative T3SS effectors (T3Es) of <i>Bradyrhizobium vignae</i> ORS3257. In the present study, we experimentally extend this catalog of T3Es by using a multi-omics approach. Transcriptome analysis under non-inducing and inducing conditions in the ORS3257 wild-type strain and the <i>ttsI</i> mutant revealed that the expression of 18 out of the 27 putative effectors previously identified, is under the control of TtsI, the global transcriptional regulator of T3SS and T3Es. Quantitative shotgun proteome analysis of culture supernatant in the wild type and <i>T3SS</i> mutant strains confirmed that 15 of the previously determined candidate T3Es are secreted by the T3SS. Moreover, the combined approaches identified nine additional putative T3Es and one of them was experimentally validated as a novel effector. Our study underscores the power of combined proteome and transcriptome analyses to complement in silico predictions and produce nearly complete effector catalogs. The establishment of the ORS3257 effectome will form the basis for a full appraisal of the symbiotic properties of this strain during its interaction with various host legumes via different processes.Nicolas BussetDjamel GullyAlbin TeuletJoël FardouxAlicia CamuelDavid CornuDany SeveracEric GiraudPeter MergaertMDPI AGarticle<i>Bradyrhizobium</i>type III secretion systemeffectornodulationlegume symbiosisnod factorMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1592, p 1592 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Bradyrhizobium</i> type III secretion system effector nodulation legume symbiosis nod factor Microbiology QR1-502
spellingShingle	<i>Bradyrhizobium</i> type III secretion system effector nodulation legume symbiosis nod factor Microbiology QR1-502 Nicolas Busset Djamel Gully Albin Teulet Joël Fardoux Alicia Camuel David Cornu Dany Severac Eric Giraud Peter Mergaert The Type III Effectome of the Symbiotic <i>Bradyrhizobium</i> <i>vignae</i> Strain ORS3257
description	Many <i>Bradyrhizobium</i> strains are able to establish a Nod factor-independent symbiosis with the leguminous plant <i>Aeschynomene indica</i> by the use of a type III secretion system (T3SS). Recently, an important advance in the understanding of the molecular factors supporting this symbiosis has been achieved by the in silico identification and functional characterization of 27 putative T3SS effectors (T3Es) of <i>Bradyrhizobium vignae</i> ORS3257. In the present study, we experimentally extend this catalog of T3Es by using a multi-omics approach. Transcriptome analysis under non-inducing and inducing conditions in the ORS3257 wild-type strain and the <i>ttsI</i> mutant revealed that the expression of 18 out of the 27 putative effectors previously identified, is under the control of TtsI, the global transcriptional regulator of T3SS and T3Es. Quantitative shotgun proteome analysis of culture supernatant in the wild type and <i>T3SS</i> mutant strains confirmed that 15 of the previously determined candidate T3Es are secreted by the T3SS. Moreover, the combined approaches identified nine additional putative T3Es and one of them was experimentally validated as a novel effector. Our study underscores the power of combined proteome and transcriptome analyses to complement in silico predictions and produce nearly complete effector catalogs. The establishment of the ORS3257 effectome will form the basis for a full appraisal of the symbiotic properties of this strain during its interaction with various host legumes via different processes.
format	article
author	Nicolas Busset Djamel Gully Albin Teulet Joël Fardoux Alicia Camuel David Cornu Dany Severac Eric Giraud Peter Mergaert
author_facet	Nicolas Busset Djamel Gully Albin Teulet Joël Fardoux Alicia Camuel David Cornu Dany Severac Eric Giraud Peter Mergaert
author_sort	Nicolas Busset
title	The Type III Effectome of the Symbiotic <i>Bradyrhizobium</i> <i>vignae</i> Strain ORS3257
title_short	The Type III Effectome of the Symbiotic <i>Bradyrhizobium</i> <i>vignae</i> Strain ORS3257
title_full	The Type III Effectome of the Symbiotic <i>Bradyrhizobium</i> <i>vignae</i> Strain ORS3257
title_fullStr	The Type III Effectome of the Symbiotic <i>Bradyrhizobium</i> <i>vignae</i> Strain ORS3257
title_full_unstemmed	The Type III Effectome of the Symbiotic <i>Bradyrhizobium</i> <i>vignae</i> Strain ORS3257
title_sort	type iii effectome of the symbiotic <i>bradyrhizobium</i> <i>vignae</i> strain ors3257
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d4c2837411724f72a0442f57a4d63c35
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The Type III Effectome of the Symbiotic <i>Bradyrhizobium</i> <i>vignae</i> Strain ORS3257

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