Preparation, Characterization and Evaluation of Guar Films Impregnated with Relaxing Peptide Loaded into Chitosan Microparticles

Background: Biological molecules such as therapeutic proteins and peptides have provided ample opportunities for further improvements in health and wellbeing. However, such biomacromolecules face stability challenges regarding premature processing and digestion, whenever any enteric delivery route i...

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Autores principales:	Patrícia Batista, Pedro M. Castro, Ana Raquel Madureira, Bruno Sarmento, Manuela Pintado
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bioactive peptides oral delivery system chitosan oral films Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/d4ce6b091693428d94a20f19796005d4
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spelling	oai:doaj.org-article:d4ce6b091693428d94a20f19796005d42021-11-11T14:58:59ZPreparation, Characterization and Evaluation of Guar Films Impregnated with Relaxing Peptide Loaded into Chitosan Microparticles10.3390/app112198492076-3417https://doaj.org/article/d4ce6b091693428d94a20f19796005d42021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/9849https://doaj.org/toc/2076-3417Background: Biological molecules such as therapeutic proteins and peptides have provided ample opportunities for further improvements in health and wellbeing. However, such biomacromolecules face stability challenges regarding premature processing and digestion, whenever any enteric delivery route is considered. Hence, oral guar films entrapping peptide-containing chitosan microparticles have been developed as a new delivery system to carry and protect therapeutic relaxing peptides. Results: The composite films were characterized regarding physicochemical properties using FTIR as well as peptide encapsulation efficiency, cytotoxicity, and peptide permeability through buccal and intestinal cell-based models. The composite films with 0.18% (<i>w/w</i>) of peptide demonstrated ca. 0.63% of peptide loading efficiency into the chitosan microparticles and to be safe for buccal and intestinal epithelial cells up to 24 h of contact. The permeability assays through TR146 cells to mimic buccal mucosa epithelium and Caco-2/HT29-MTX to mimic duodenum conditions showed that the incorporation of peptide chitosan microparticles into guar-gum oral films offered a faster permeability, when compared with the free peptide and peptide-chitosan-microparticles-loaded chitosan. Conclusion: This study suggests that peptide-loaded chitosan microparticles into guar-gum oral films might be a promising carrier for enhancement of the buccal and intestinal absorption of a relaxing peptide.Patrícia BatistaPedro M. CastroAna Raquel MadureiraBruno SarmentoManuela PintadoMDPI AGarticlebioactive peptidesoral delivery systemchitosanoral filmsTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 9849, p 9849 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioactive peptides oral delivery system chitosan oral films Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	bioactive peptides oral delivery system chitosan oral films Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Patrícia Batista Pedro M. Castro Ana Raquel Madureira Bruno Sarmento Manuela Pintado Preparation, Characterization and Evaluation of Guar Films Impregnated with Relaxing Peptide Loaded into Chitosan Microparticles
description	Background: Biological molecules such as therapeutic proteins and peptides have provided ample opportunities for further improvements in health and wellbeing. However, such biomacromolecules face stability challenges regarding premature processing and digestion, whenever any enteric delivery route is considered. Hence, oral guar films entrapping peptide-containing chitosan microparticles have been developed as a new delivery system to carry and protect therapeutic relaxing peptides. Results: The composite films were characterized regarding physicochemical properties using FTIR as well as peptide encapsulation efficiency, cytotoxicity, and peptide permeability through buccal and intestinal cell-based models. The composite films with 0.18% (<i>w/w</i>) of peptide demonstrated ca. 0.63% of peptide loading efficiency into the chitosan microparticles and to be safe for buccal and intestinal epithelial cells up to 24 h of contact. The permeability assays through TR146 cells to mimic buccal mucosa epithelium and Caco-2/HT29-MTX to mimic duodenum conditions showed that the incorporation of peptide chitosan microparticles into guar-gum oral films offered a faster permeability, when compared with the free peptide and peptide-chitosan-microparticles-loaded chitosan. Conclusion: This study suggests that peptide-loaded chitosan microparticles into guar-gum oral films might be a promising carrier for enhancement of the buccal and intestinal absorption of a relaxing peptide.
format	article
author	Patrícia Batista Pedro M. Castro Ana Raquel Madureira Bruno Sarmento Manuela Pintado
author_facet	Patrícia Batista Pedro M. Castro Ana Raquel Madureira Bruno Sarmento Manuela Pintado
author_sort	Patrícia Batista
title	Preparation, Characterization and Evaluation of Guar Films Impregnated with Relaxing Peptide Loaded into Chitosan Microparticles
title_short	Preparation, Characterization and Evaluation of Guar Films Impregnated with Relaxing Peptide Loaded into Chitosan Microparticles
title_full	Preparation, Characterization and Evaluation of Guar Films Impregnated with Relaxing Peptide Loaded into Chitosan Microparticles
title_fullStr	Preparation, Characterization and Evaluation of Guar Films Impregnated with Relaxing Peptide Loaded into Chitosan Microparticles
title_full_unstemmed	Preparation, Characterization and Evaluation of Guar Films Impregnated with Relaxing Peptide Loaded into Chitosan Microparticles
title_sort	preparation, characterization and evaluation of guar films impregnated with relaxing peptide loaded into chitosan microparticles
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d4ce6b091693428d94a20f19796005d4
work_keys_str_mv	AT patriciabatista preparationcharacterizationandevaluationofguarfilmsimpregnatedwithrelaxingpeptideloadedintochitosanmicroparticles AT pedromcastro preparationcharacterizationandevaluationofguarfilmsimpregnatedwithrelaxingpeptideloadedintochitosanmicroparticles AT anaraquelmadureira preparationcharacterizationandevaluationofguarfilmsimpregnatedwithrelaxingpeptideloadedintochitosanmicroparticles AT brunosarmento preparationcharacterizationandevaluationofguarfilmsimpregnatedwithrelaxingpeptideloadedintochitosanmicroparticles AT manuelapintado preparationcharacterizationandevaluationofguarfilmsimpregnatedwithrelaxingpeptideloadedintochitosanmicroparticles
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Preparation, Characterization and Evaluation of Guar Films Impregnated with Relaxing Peptide Loaded into Chitosan Microparticles

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