Osteoblast-osteoclast co-cultures: A systematic review and map of available literature

Drug research with animal models is expensive, time-consuming and translation to clinical trials is often poor, resulting in a desire to replace, reduce, and refine the use of animal models. One approach to replace and reduce the use of animal models is to use in vitro cell-culture models. To study...

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Autores principales:	Stefan J. A. Remmers, Bregje W. M. de Wildt, Michelle A. M. Vis, Eva S. R. Spaander, Rob B. M. de Vries, Keita Ito, Sandra Hofmann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d4cf8223d8ac4219818a97ac934763f5
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spelling	oai:doaj.org-article:d4cf8223d8ac4219818a97ac934763f52021-11-11T07:14:39ZOsteoblast-osteoclast co-cultures: A systematic review and map of available literature1932-6203https://doaj.org/article/d4cf8223d8ac4219818a97ac934763f52021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568160/?tool=EBIhttps://doaj.org/toc/1932-6203Drug research with animal models is expensive, time-consuming and translation to clinical trials is often poor, resulting in a desire to replace, reduce, and refine the use of animal models. One approach to replace and reduce the use of animal models is to use in vitro cell-culture models. To study bone physiology, bone diseases and drugs, many studies have been published using osteoblast-osteoclast co-cultures. The use of osteoblast-osteoclast co-cultures is usually not clearly mentioned in the title and abstract, making it difficult to identify these studies without a systematic search and thorough review. As a result, researchers are all developing their own methods, leading to conceptually similar studies with many methodological differences and, as a consequence, incomparable results. The aim of this study was to systematically review existing osteoblast-osteoclast co-culture studies published up to 6 January 2020, and to give an overview of their methods, predetermined outcome measures (formation and resorption, and ALP and TRAP quantification as surrogate markers for formation and resorption, respectively), and other useful parameters for analysis. Information regarding these outcome measures was extracted and collected in a database, and each study was further evaluated on whether both the osteoblasts and osteoclasts were analyzed using relevant outcome measures. From these studies, additional details on methods, cells and culture conditions were extracted into a second database to allow searching on more characteristics. The two databases presented in this publication provide an unprecedented amount of information on cells, culture conditions and analytical techniques for using and studying osteoblast-osteoclast co-cultures. They allow researchers to identify publications relevant to their specific needs and allow easy validation and comparison with existing literature. Finally, we provide the information and tools necessary for others to use, manipulate and expand the databases for their needs.Stefan J. A. RemmersBregje W. M. de WildtMichelle A. M. VisEva S. R. SpaanderRob B. M. de VriesKeita ItoSandra HofmannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefan J. A. Remmers Bregje W. M. de Wildt Michelle A. M. Vis Eva S. R. Spaander Rob B. M. de Vries Keita Ito Sandra Hofmann Osteoblast-osteoclast co-cultures: A systematic review and map of available literature
description	Drug research with animal models is expensive, time-consuming and translation to clinical trials is often poor, resulting in a desire to replace, reduce, and refine the use of animal models. One approach to replace and reduce the use of animal models is to use in vitro cell-culture models. To study bone physiology, bone diseases and drugs, many studies have been published using osteoblast-osteoclast co-cultures. The use of osteoblast-osteoclast co-cultures is usually not clearly mentioned in the title and abstract, making it difficult to identify these studies without a systematic search and thorough review. As a result, researchers are all developing their own methods, leading to conceptually similar studies with many methodological differences and, as a consequence, incomparable results. The aim of this study was to systematically review existing osteoblast-osteoclast co-culture studies published up to 6 January 2020, and to give an overview of their methods, predetermined outcome measures (formation and resorption, and ALP and TRAP quantification as surrogate markers for formation and resorption, respectively), and other useful parameters for analysis. Information regarding these outcome measures was extracted and collected in a database, and each study was further evaluated on whether both the osteoblasts and osteoclasts were analyzed using relevant outcome measures. From these studies, additional details on methods, cells and culture conditions were extracted into a second database to allow searching on more characteristics. The two databases presented in this publication provide an unprecedented amount of information on cells, culture conditions and analytical techniques for using and studying osteoblast-osteoclast co-cultures. They allow researchers to identify publications relevant to their specific needs and allow easy validation and comparison with existing literature. Finally, we provide the information and tools necessary for others to use, manipulate and expand the databases for their needs.
format	article
author	Stefan J. A. Remmers Bregje W. M. de Wildt Michelle A. M. Vis Eva S. R. Spaander Rob B. M. de Vries Keita Ito Sandra Hofmann
author_facet	Stefan J. A. Remmers Bregje W. M. de Wildt Michelle A. M. Vis Eva S. R. Spaander Rob B. M. de Vries Keita Ito Sandra Hofmann
author_sort	Stefan J. A. Remmers
title	Osteoblast-osteoclast co-cultures: A systematic review and map of available literature
title_short	Osteoblast-osteoclast co-cultures: A systematic review and map of available literature
title_full	Osteoblast-osteoclast co-cultures: A systematic review and map of available literature
title_fullStr	Osteoblast-osteoclast co-cultures: A systematic review and map of available literature
title_full_unstemmed	Osteoblast-osteoclast co-cultures: A systematic review and map of available literature
title_sort	osteoblast-osteoclast co-cultures: a systematic review and map of available literature
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/d4cf8223d8ac4219818a97ac934763f5
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Osteoblast-osteoclast co-cultures: A systematic review and map of available literature

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