UVB protective effects of Sargassum horneri through the regulation of Nrf2 mediated antioxidant mechanism

Abstract The present study aimed to evaluate the protective effect of a methanol extract of Sargassum horneri (SHM), which contains 6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one (HTT) and apo-9′-fucoxanthinone, against ultraviolet B (UVB)-induced cellular damage in human keratin...

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Autores principales:	Eui Jeong Han, Seo-Young Kim, Hee-Jin Han, Hyun-Soo Kim, Kil-Nam Kim, Ilekuttige Priyan Shanura Fernando, Disanayake Mudiyanselage Dinesh Madusanka, Mawalle Kankanamge Hasitha Madhawa Dias, Sun Hee Cheong, Sang Rul Park, Young Seok Han, Kyounghoon Lee, Ginnae Ahn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d4d8c854466c4a1883217ae28e23effe
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spelling	oai:doaj.org-article:d4d8c854466c4a1883217ae28e23effe2021-12-02T17:01:57ZUVB protective effects of Sargassum horneri through the regulation of Nrf2 mediated antioxidant mechanism10.1038/s41598-021-88949-32045-2322https://doaj.org/article/d4d8c854466c4a1883217ae28e23effe2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88949-3https://doaj.org/toc/2045-2322Abstract The present study aimed to evaluate the protective effect of a methanol extract of Sargassum horneri (SHM), which contains 6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one (HTT) and apo-9′-fucoxanthinone, against ultraviolet B (UVB)-induced cellular damage in human keratinocytes and its underlying mechanism. SHM significantly improved cell viability of UVB-exposed human keratinocytes by reducing the generation of intracellular reactive oxygen species (ROS). Moreover, SHM inhibited UVB exposure-induced apoptosis by reducing the formation of apoptotic bodies and the populations of the sub-G1 hypodiploid cells and the early apoptotic cells by modulating the expression of the anti- and pro-apoptotic molecules, Bcl-2 and Bax, respectively. Furthermore, SHM inhibited NF-κB p65 activation by inducing the activation of Nrf2/HO-1 signaling. The cytoprotective and antiapoptotic activities of SHM are abolished by the inhibition of HO-1 signaling. In further study, SHM restored the skin dryness and skin barrier disruption in UVB-exposed human keratinocytes. Based to these results, our study suggests that SHM protects the cells against UVB-induced cellular damages through the Nrf2/HO-1/NF-κB p65 signaling pathway and may be potentially useful for the prevention of UVB-induced skin damage.Eui Jeong HanSeo-Young KimHee-Jin HanHyun-Soo KimKil-Nam KimIlekuttige Priyan Shanura FernandoDisanayake Mudiyanselage Dinesh MadusankaMawalle Kankanamge Hasitha Madhawa DiasSun Hee CheongSang Rul ParkYoung Seok HanKyounghoon LeeGinnae AhnNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eui Jeong Han Seo-Young Kim Hee-Jin Han Hyun-Soo Kim Kil-Nam Kim Ilekuttige Priyan Shanura Fernando Disanayake Mudiyanselage Dinesh Madusanka Mawalle Kankanamge Hasitha Madhawa Dias Sun Hee Cheong Sang Rul Park Young Seok Han Kyounghoon Lee Ginnae Ahn UVB protective effects of Sargassum horneri through the regulation of Nrf2 mediated antioxidant mechanism
description	Abstract The present study aimed to evaluate the protective effect of a methanol extract of Sargassum horneri (SHM), which contains 6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one (HTT) and apo-9′-fucoxanthinone, against ultraviolet B (UVB)-induced cellular damage in human keratinocytes and its underlying mechanism. SHM significantly improved cell viability of UVB-exposed human keratinocytes by reducing the generation of intracellular reactive oxygen species (ROS). Moreover, SHM inhibited UVB exposure-induced apoptosis by reducing the formation of apoptotic bodies and the populations of the sub-G1 hypodiploid cells and the early apoptotic cells by modulating the expression of the anti- and pro-apoptotic molecules, Bcl-2 and Bax, respectively. Furthermore, SHM inhibited NF-κB p65 activation by inducing the activation of Nrf2/HO-1 signaling. The cytoprotective and antiapoptotic activities of SHM are abolished by the inhibition of HO-1 signaling. In further study, SHM restored the skin dryness and skin barrier disruption in UVB-exposed human keratinocytes. Based to these results, our study suggests that SHM protects the cells against UVB-induced cellular damages through the Nrf2/HO-1/NF-κB p65 signaling pathway and may be potentially useful for the prevention of UVB-induced skin damage.
format	article
author	Eui Jeong Han Seo-Young Kim Hee-Jin Han Hyun-Soo Kim Kil-Nam Kim Ilekuttige Priyan Shanura Fernando Disanayake Mudiyanselage Dinesh Madusanka Mawalle Kankanamge Hasitha Madhawa Dias Sun Hee Cheong Sang Rul Park Young Seok Han Kyounghoon Lee Ginnae Ahn
author_facet	Eui Jeong Han Seo-Young Kim Hee-Jin Han Hyun-Soo Kim Kil-Nam Kim Ilekuttige Priyan Shanura Fernando Disanayake Mudiyanselage Dinesh Madusanka Mawalle Kankanamge Hasitha Madhawa Dias Sun Hee Cheong Sang Rul Park Young Seok Han Kyounghoon Lee Ginnae Ahn
author_sort	Eui Jeong Han
title	UVB protective effects of Sargassum horneri through the regulation of Nrf2 mediated antioxidant mechanism
title_short	UVB protective effects of Sargassum horneri through the regulation of Nrf2 mediated antioxidant mechanism
title_full	UVB protective effects of Sargassum horneri through the regulation of Nrf2 mediated antioxidant mechanism
title_fullStr	UVB protective effects of Sargassum horneri through the regulation of Nrf2 mediated antioxidant mechanism
title_full_unstemmed	UVB protective effects of Sargassum horneri through the regulation of Nrf2 mediated antioxidant mechanism
title_sort	uvb protective effects of sargassum horneri through the regulation of nrf2 mediated antioxidant mechanism
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d4d8c854466c4a1883217ae28e23effe
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UVB protective effects of Sargassum horneri through the regulation of Nrf2 mediated antioxidant mechanism

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