Association Study of Symbiotic Genes in Pea (<i>Pisum sativum</i> L.) Cultivars Grown in Symbiotic Conditions

In garden pea (<i>Pisum sativum</i> L.), several symbiotic genes are known to control the development of mutualistic symbioses with nodule bacteria (NB) and arbuscular mycorrhizal fungi (AMF). Here, we studied whether the allelic state of the symbiotic genes was associated with the growt...

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Autores principales:	Vladimir A. Zhukov, Aleksandr I. Zhernakov, Anton S. Sulima, Olga A. Kulaeva, Marina S. Kliukova, Alexey M. Afonin, Oksana Y. Shtark, Igor A. Tikhonovich
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Pisum sativum</i> symbiotic genes nodule bacteria arbuscular mycorrhiza symbiotic responsivity symbiotic effectiveness Agriculture S
Acceso en línea:	https://doaj.org/article/d4eed3f05b804d4ab59b8099722ae618
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spelling	oai:doaj.org-article:d4eed3f05b804d4ab59b8099722ae6182021-11-25T16:12:47ZAssociation Study of Symbiotic Genes in Pea (<i>Pisum sativum</i> L.) Cultivars Grown in Symbiotic Conditions10.3390/agronomy111123682073-4395https://doaj.org/article/d4eed3f05b804d4ab59b8099722ae6182021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2368https://doaj.org/toc/2073-4395In garden pea (<i>Pisum sativum</i> L.), several symbiotic genes are known to control the development of mutualistic symbioses with nodule bacteria (NB) and arbuscular mycorrhizal fungi (AMF). Here, we studied whether the allelic state of the symbiotic genes was associated with the growth parameters of pea plants under single inoculation with NB and under double inoculation with NB + AMF. Using different statistical methods, we analyzed the dataset obtained from a pot experiment that involved 99 pea cultivars, 10 of which were characterized as having shortened internodes due to the presence of the natural mutation p.A229T in the developmental gene <i>Le</i>. The plant’s habitus strongly influenced most of the studied growth and yield parameters and the effectiveness of the symbiotic interactions under NB and NB + AMF inoculation. Double inoculation had different effects on <i>Le</i><sup>+</sup> (normal) and <i>le</i><sup>−</sup> (dwarf) plants with regard to nitrogen and phosphorus content in seeds. Regardless of the <i>Le</i>-status of plants, allelic states of the symbiotic gene <i>LykX</i> encoding the putative receptor of Nod factors (bacterial signal molecules) were shown to be associated with seed number, thousand-seed weight, and pod number at the level of FDR < 0.001, whereas associations of allelic states of the other studied symbiotic genes were less significant.Vladimir A. ZhukovAleksandr I. ZhernakovAnton S. SulimaOlga A. KulaevaMarina S. KliukovaAlexey M. AfoninOksana Y. ShtarkIgor A. TikhonovichMDPI AGarticle<i>Pisum sativum</i>symbiotic genesnodule bacteriaarbuscular mycorrhizasymbiotic responsivitysymbiotic effectivenessAgricultureSENAgronomy, Vol 11, Iss 2368, p 2368 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Pisum sativum</i> symbiotic genes nodule bacteria arbuscular mycorrhiza symbiotic responsivity symbiotic effectiveness Agriculture S
spellingShingle	<i>Pisum sativum</i> symbiotic genes nodule bacteria arbuscular mycorrhiza symbiotic responsivity symbiotic effectiveness Agriculture S Vladimir A. Zhukov Aleksandr I. Zhernakov Anton S. Sulima Olga A. Kulaeva Marina S. Kliukova Alexey M. Afonin Oksana Y. Shtark Igor A. Tikhonovich Association Study of Symbiotic Genes in Pea (<i>Pisum sativum</i> L.) Cultivars Grown in Symbiotic Conditions
description	In garden pea (<i>Pisum sativum</i> L.), several symbiotic genes are known to control the development of mutualistic symbioses with nodule bacteria (NB) and arbuscular mycorrhizal fungi (AMF). Here, we studied whether the allelic state of the symbiotic genes was associated with the growth parameters of pea plants under single inoculation with NB and under double inoculation with NB + AMF. Using different statistical methods, we analyzed the dataset obtained from a pot experiment that involved 99 pea cultivars, 10 of which were characterized as having shortened internodes due to the presence of the natural mutation p.A229T in the developmental gene <i>Le</i>. The plant’s habitus strongly influenced most of the studied growth and yield parameters and the effectiveness of the symbiotic interactions under NB and NB + AMF inoculation. Double inoculation had different effects on <i>Le</i><sup>+</sup> (normal) and <i>le</i><sup>−</sup> (dwarf) plants with regard to nitrogen and phosphorus content in seeds. Regardless of the <i>Le</i>-status of plants, allelic states of the symbiotic gene <i>LykX</i> encoding the putative receptor of Nod factors (bacterial signal molecules) were shown to be associated with seed number, thousand-seed weight, and pod number at the level of FDR < 0.001, whereas associations of allelic states of the other studied symbiotic genes were less significant.
format	article
author	Vladimir A. Zhukov Aleksandr I. Zhernakov Anton S. Sulima Olga A. Kulaeva Marina S. Kliukova Alexey M. Afonin Oksana Y. Shtark Igor A. Tikhonovich
author_facet	Vladimir A. Zhukov Aleksandr I. Zhernakov Anton S. Sulima Olga A. Kulaeva Marina S. Kliukova Alexey M. Afonin Oksana Y. Shtark Igor A. Tikhonovich
author_sort	Vladimir A. Zhukov
title	Association Study of Symbiotic Genes in Pea (<i>Pisum sativum</i> L.) Cultivars Grown in Symbiotic Conditions
title_short	Association Study of Symbiotic Genes in Pea (<i>Pisum sativum</i> L.) Cultivars Grown in Symbiotic Conditions
title_full	Association Study of Symbiotic Genes in Pea (<i>Pisum sativum</i> L.) Cultivars Grown in Symbiotic Conditions
title_fullStr	Association Study of Symbiotic Genes in Pea (<i>Pisum sativum</i> L.) Cultivars Grown in Symbiotic Conditions
title_full_unstemmed	Association Study of Symbiotic Genes in Pea (<i>Pisum sativum</i> L.) Cultivars Grown in Symbiotic Conditions
title_sort	association study of symbiotic genes in pea (<i>pisum sativum</i> l.) cultivars grown in symbiotic conditions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d4eed3f05b804d4ab59b8099722ae618
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Association Study of Symbiotic Genes in Pea (<i>Pisum sativum</i> L.) Cultivars Grown in Symbiotic Conditions

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