A handheld platform for target protein detection and quantification using disposable nanopore strips

Abstract Accessible point-of-care technologies that can provide immunoassay and molecular modalities could dramatically enhance diagnostics, particularly for infectious disease control in low-resource settings. Solid-state nanopores are simple and durable sensors with low-energy instrumentation requ...

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Autores principales:	Trevor J. Morin, William L. McKenna, Tyler D. Shropshire, Dustin A. Wride, Joshua D. Deschamps, Xu Liu, Reto Stamm, Hongyun Wang, William B. Dunbar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Tetanus Toxin Point-of-care Technology Detection Reagents Nanopore Experiments Lateral Flow Immunoassay (LFAs) Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d4eef1c81879446f87836983db6d0976
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spelling	oai:doaj.org-article:d4eef1c81879446f87836983db6d09762021-12-02T15:08:05ZA handheld platform for target protein detection and quantification using disposable nanopore strips10.1038/s41598-018-33086-72045-2322https://doaj.org/article/d4eef1c81879446f87836983db6d09762018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33086-7https://doaj.org/toc/2045-2322Abstract Accessible point-of-care technologies that can provide immunoassay and molecular modalities could dramatically enhance diagnostics, particularly for infectious disease control in low-resource settings. Solid-state nanopores are simple and durable sensors with low-energy instrumentation requirements. While nanopore sensors have demonstrated efficacy for nucleic acid targets, selective detection and quantification of target proteins from sample background has not been demonstrated. We present a simple approach for electronic detection and quantification of target proteins that combines novel biomolecular engineering methods, a portable reader device and disposable nanopore test strips. The target of interest can be varied by swapping the binding domain on our engineered detection reagent, which eficiently binds in the bulk-phase to the target and subsequently generates a unique signature when passing through the pore. We show modularity of the detection reagent for two HIV antibodies, TNFα and tetanus toxin as targets. A saliva swab-to-result is demonstrated for clinically relevant HIV antibody levels (0.4–20 mg/liter) in under 60 seconds. While other strip-like assays are qualitative, the presented method is quantitative and sets the stage for simultaneous immunoassay and molecular diagnostic functionality within a single portable platform.Trevor J. MorinWilliam L. McKennaTyler D. ShropshireDustin A. WrideJoshua D. DeschampsXu LiuReto StammHongyun WangWilliam B. DunbarNature PortfolioarticleTetanus ToxinPoint-of-care TechnologyDetection ReagentsNanopore ExperimentsLateral Flow Immunoassay (LFAs)MedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Tetanus Toxin Point-of-care Technology Detection Reagents Nanopore Experiments Lateral Flow Immunoassay (LFAs) Medicine R Science Q
spellingShingle	Tetanus Toxin Point-of-care Technology Detection Reagents Nanopore Experiments Lateral Flow Immunoassay (LFAs) Medicine R Science Q Trevor J. Morin William L. McKenna Tyler D. Shropshire Dustin A. Wride Joshua D. Deschamps Xu Liu Reto Stamm Hongyun Wang William B. Dunbar A handheld platform for target protein detection and quantification using disposable nanopore strips
description	Abstract Accessible point-of-care technologies that can provide immunoassay and molecular modalities could dramatically enhance diagnostics, particularly for infectious disease control in low-resource settings. Solid-state nanopores are simple and durable sensors with low-energy instrumentation requirements. While nanopore sensors have demonstrated efficacy for nucleic acid targets, selective detection and quantification of target proteins from sample background has not been demonstrated. We present a simple approach for electronic detection and quantification of target proteins that combines novel biomolecular engineering methods, a portable reader device and disposable nanopore test strips. The target of interest can be varied by swapping the binding domain on our engineered detection reagent, which eficiently binds in the bulk-phase to the target and subsequently generates a unique signature when passing through the pore. We show modularity of the detection reagent for two HIV antibodies, TNFα and tetanus toxin as targets. A saliva swab-to-result is demonstrated for clinically relevant HIV antibody levels (0.4–20 mg/liter) in under 60 seconds. While other strip-like assays are qualitative, the presented method is quantitative and sets the stage for simultaneous immunoassay and molecular diagnostic functionality within a single portable platform.
format	article
author	Trevor J. Morin William L. McKenna Tyler D. Shropshire Dustin A. Wride Joshua D. Deschamps Xu Liu Reto Stamm Hongyun Wang William B. Dunbar
author_facet	Trevor J. Morin William L. McKenna Tyler D. Shropshire Dustin A. Wride Joshua D. Deschamps Xu Liu Reto Stamm Hongyun Wang William B. Dunbar
author_sort	Trevor J. Morin
title	A handheld platform for target protein detection and quantification using disposable nanopore strips
title_short	A handheld platform for target protein detection and quantification using disposable nanopore strips
title_full	A handheld platform for target protein detection and quantification using disposable nanopore strips
title_fullStr	A handheld platform for target protein detection and quantification using disposable nanopore strips
title_full_unstemmed	A handheld platform for target protein detection and quantification using disposable nanopore strips
title_sort	handheld platform for target protein detection and quantification using disposable nanopore strips
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/d4eef1c81879446f87836983db6d0976
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A handheld platform for target protein detection and quantification using disposable nanopore strips

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