Low concentration of silver nanoparticles not only enhances the activity of horseradish peroxidase but alter the structure also.

Chemical synthesis of Ag-NPs was carried out using reduction method. The reduction mechanistic approach of silver ions was found to be a basic clue for the formation of the Ag-NPs. The nanoparticles were characterized by UV-vis, FT-IR and TEM analysis. We had designed some experiments in support of...

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Autores principales:	Zoheb Karim, Rohana Adnan, Mohd Saquib Ansari
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d4f57f1fb32745d89b2d4758c4d6b1a3
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spelling	oai:doaj.org-article:d4f57f1fb32745d89b2d4758c4d6b1a32021-11-18T07:10:45ZLow concentration of silver nanoparticles not only enhances the activity of horseradish peroxidase but alter the structure also.1932-620310.1371/journal.pone.0041422https://doaj.org/article/d4f57f1fb32745d89b2d4758c4d6b1a32012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22848490/?tool=EBIhttps://doaj.org/toc/1932-6203Chemical synthesis of Ag-NPs was carried out using reduction method. The reduction mechanistic approach of silver ions was found to be a basic clue for the formation of the Ag-NPs. The nanoparticles were characterized by UV-vis, FT-IR and TEM analysis. We had designed some experiments in support of our hypothesis, "low concentrations of novel nanoparticles (silver and gold) increases the activity of plant peroxidases and alter their structure also", we had used Ag-NPs and HRP as models. The immobilization/interaction experiment had demonstrated the specific concentration range of the Ag-NPs and within this range, an increase in HRP activity was reported. At 0.08 mM concentration of Ag-NPs, 50% increase in the activity yield was found. The U.V-vis spectra had demonstrated the increase in the absorbance of HRP within the reported concentration range (0.06-0.12 mM). Above and below this concentration range there was a decrease in the activity of HRP. The results that we had found from the fluorescence spectra were also in favor of our hypothesis. There was a maximum increase in ellipticity and α-helix contents in the presence of 0.08 mM concentration of Ag-NPs, demonstrated by circular dichroism (CD) spectra. Finally, incubation of a plant peroxidase, HRP with Ag-NPs, within the reported concentration range not only enhances the activity but also alter the structure.Zoheb KarimRohana AdnanMohd Saquib AnsariPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e41422 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zoheb Karim Rohana Adnan Mohd Saquib Ansari Low concentration of silver nanoparticles not only enhances the activity of horseradish peroxidase but alter the structure also.
description	Chemical synthesis of Ag-NPs was carried out using reduction method. The reduction mechanistic approach of silver ions was found to be a basic clue for the formation of the Ag-NPs. The nanoparticles were characterized by UV-vis, FT-IR and TEM analysis. We had designed some experiments in support of our hypothesis, "low concentrations of novel nanoparticles (silver and gold) increases the activity of plant peroxidases and alter their structure also", we had used Ag-NPs and HRP as models. The immobilization/interaction experiment had demonstrated the specific concentration range of the Ag-NPs and within this range, an increase in HRP activity was reported. At 0.08 mM concentration of Ag-NPs, 50% increase in the activity yield was found. The U.V-vis spectra had demonstrated the increase in the absorbance of HRP within the reported concentration range (0.06-0.12 mM). Above and below this concentration range there was a decrease in the activity of HRP. The results that we had found from the fluorescence spectra were also in favor of our hypothesis. There was a maximum increase in ellipticity and α-helix contents in the presence of 0.08 mM concentration of Ag-NPs, demonstrated by circular dichroism (CD) spectra. Finally, incubation of a plant peroxidase, HRP with Ag-NPs, within the reported concentration range not only enhances the activity but also alter the structure.
format	article
author	Zoheb Karim Rohana Adnan Mohd Saquib Ansari
author_facet	Zoheb Karim Rohana Adnan Mohd Saquib Ansari
author_sort	Zoheb Karim
title	Low concentration of silver nanoparticles not only enhances the activity of horseradish peroxidase but alter the structure also.
title_short	Low concentration of silver nanoparticles not only enhances the activity of horseradish peroxidase but alter the structure also.
title_full	Low concentration of silver nanoparticles not only enhances the activity of horseradish peroxidase but alter the structure also.
title_fullStr	Low concentration of silver nanoparticles not only enhances the activity of horseradish peroxidase but alter the structure also.
title_full_unstemmed	Low concentration of silver nanoparticles not only enhances the activity of horseradish peroxidase but alter the structure also.
title_sort	low concentration of silver nanoparticles not only enhances the activity of horseradish peroxidase but alter the structure also.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d4f57f1fb32745d89b2d4758c4d6b1a3
work_keys_str_mv	AT zohebkarim lowconcentrationofsilvernanoparticlesnotonlyenhancestheactivityofhorseradishperoxidasebutalterthestructurealso AT rohanaadnan lowconcentrationofsilvernanoparticlesnotonlyenhancestheactivityofhorseradishperoxidasebutalterthestructurealso AT mohdsaquibansari lowconcentrationofsilvernanoparticlesnotonlyenhancestheactivityofhorseradishperoxidasebutalterthestructurealso
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Low concentration of silver nanoparticles not only enhances the activity of horseradish peroxidase but alter the structure also.

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