Selective control of the contact and transport between droplet pairs by electrowetting-on-dielectric for droplet-array sandwiching technology

Abstract Methodological advances in on-chip technology enable high-throughput drug screening, such as droplet-array sandwiching technology. Droplet-array sandwiching technology involves upper and lower substrates with a droplet-array designed for a one-step process. This technology is, however, limi...

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Autores principales:	Satoshi Konishi, Chikara Ohya, Tatsuhiro Yamada
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d502e4103534490cac1b69929afec10a
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spelling	oai:doaj.org-article:d502e4103534490cac1b69929afec10a2021-12-02T17:52:32ZSelective control of the contact and transport between droplet pairs by electrowetting-on-dielectric for droplet-array sandwiching technology10.1038/s41598-021-91219-x2045-2322https://doaj.org/article/d502e4103534490cac1b69929afec10a2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91219-xhttps://doaj.org/toc/2045-2322Abstract Methodological advances in on-chip technology enable high-throughput drug screening, such as droplet-array sandwiching technology. Droplet-array sandwiching technology involves upper and lower substrates with a droplet-array designed for a one-step process. This technology is, however, limited to batch manipulation of the droplet-array. Here, we propose a method for selective control of individual droplets, which allows different conditions for individual droplet pairs. Electrowetting-on-dielectric (EWOD) technology is introduced to control the height of the droplets so that the contact between droplet-pairs can be individually controlled. Circular patterns 4 mm in diameter composed of electrodes for EWOD and hydrophilic–hydrophobic patterns for droplet formation 4 μl in volume were developed. We demonstrate the selective control of the droplet height by EWOD for an applied voltage up to 160 V and selective control of the contact and transport of substances. Presented results will provide useful method for advanced drug screening, including cell-based screening.Satoshi KonishiChikara OhyaTatsuhiro YamadaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Satoshi Konishi Chikara Ohya Tatsuhiro Yamada Selective control of the contact and transport between droplet pairs by electrowetting-on-dielectric for droplet-array sandwiching technology
description	Abstract Methodological advances in on-chip technology enable high-throughput drug screening, such as droplet-array sandwiching technology. Droplet-array sandwiching technology involves upper and lower substrates with a droplet-array designed for a one-step process. This technology is, however, limited to batch manipulation of the droplet-array. Here, we propose a method for selective control of individual droplets, which allows different conditions for individual droplet pairs. Electrowetting-on-dielectric (EWOD) technology is introduced to control the height of the droplets so that the contact between droplet-pairs can be individually controlled. Circular patterns 4 mm in diameter composed of electrodes for EWOD and hydrophilic–hydrophobic patterns for droplet formation 4 μl in volume were developed. We demonstrate the selective control of the droplet height by EWOD for an applied voltage up to 160 V and selective control of the contact and transport of substances. Presented results will provide useful method for advanced drug screening, including cell-based screening.
format	article
author	Satoshi Konishi Chikara Ohya Tatsuhiro Yamada
author_facet	Satoshi Konishi Chikara Ohya Tatsuhiro Yamada
author_sort	Satoshi Konishi
title	Selective control of the contact and transport between droplet pairs by electrowetting-on-dielectric for droplet-array sandwiching technology
title_short	Selective control of the contact and transport between droplet pairs by electrowetting-on-dielectric for droplet-array sandwiching technology
title_full	Selective control of the contact and transport between droplet pairs by electrowetting-on-dielectric for droplet-array sandwiching technology
title_fullStr	Selective control of the contact and transport between droplet pairs by electrowetting-on-dielectric for droplet-array sandwiching technology
title_full_unstemmed	Selective control of the contact and transport between droplet pairs by electrowetting-on-dielectric for droplet-array sandwiching technology
title_sort	selective control of the contact and transport between droplet pairs by electrowetting-on-dielectric for droplet-array sandwiching technology
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d502e4103534490cac1b69929afec10a
work_keys_str_mv	AT satoshikonishi selectivecontrolofthecontactandtransportbetweendropletpairsbyelectrowettingondielectricfordropletarraysandwichingtechnology AT chikaraohya selectivecontrolofthecontactandtransportbetweendropletpairsbyelectrowettingondielectricfordropletarraysandwichingtechnology AT tatsuhiroyamada selectivecontrolofthecontactandtransportbetweendropletpairsbyelectrowettingondielectricfordropletarraysandwichingtechnology
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Selective control of the contact and transport between droplet pairs by electrowetting-on-dielectric for droplet-array sandwiching technology

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