LncRNA DNAJC3-AS1 Promotes Hepatocellular Carcinoma (HCC) Progression via Sponging Premature miR-27b

Changbo Fu,1 Jianxiu Li,2 Ping Li,3 Dan Cheng4 1Department of Hepatobiliary and Pancreatic Surgery, Hubei Cancer Hospital, Wuhan City, 430000, People’s Republic of China; 2Disinfection Supply Center of Weifang Yidu Central Hospital, Weifang City, 266000, People’s Republic of China; 3Emergency Care C...

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Autores principales:	Fu C, Li J, Li P, Cheng D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	dnajc3-as1 mir-27b hepatocellular carcinoma maturation Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/d503ff161e5947ceabcda6104e0b42cd
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spelling	oai:doaj.org-article:d503ff161e5947ceabcda6104e0b42cd2021-11-16T18:47:50ZLncRNA DNAJC3-AS1 Promotes Hepatocellular Carcinoma (HCC) Progression via Sponging Premature miR-27b1179-1322https://doaj.org/article/d503ff161e5947ceabcda6104e0b42cd2021-11-01T00:00:00Zhttps://www.dovepress.com/lncrna-dnajc3-as1-promotes-hepatocellular-carcinoma-hcc-progression-vi-peer-reviewed-fulltext-article-CMARhttps://doaj.org/toc/1179-1322Changbo Fu,1 Jianxiu Li,2 Ping Li,3 Dan Cheng4 1Department of Hepatobiliary and Pancreatic Surgery, Hubei Cancer Hospital, Wuhan City, 430000, People’s Republic of China; 2Disinfection Supply Center of Weifang Yidu Central Hospital, Weifang City, 266000, People’s Republic of China; 3Emergency Care Center of Qingdao Central Hospital, Qingdao City, 266000, People’s Republic of China; 4Department of Liver Disease Infection, Edong Healthcare City Hospital of Traditional Chinese Medicine, Infections Disease Hospital, Huangshi City, 435000, People’s Republic of ChinaCorrespondence: Dan ChengDepartment of Liver Disease Infection, Edong Healthcare City Hospital of Traditional Chinese Medicine, Infections Disease Hospital, No. 634 Shenxia Road, Huangshi City, 435000, People’s Republic of ChinaEmail chengdandisease@163.comPurpose: Long non-coding RNA (lncRNA) DNAJC3 antisense RNA 1 (head to head) (DNAJC3-AS1) plays a key role in the progression of several cancers. However, its biological role in hepatocellular carcinoma (HCC) is still unclear. We aimed to investigate the role of DNAJC3-AS1 in the development of HCC and reveal the potential mechanisms.Materials and Methods: Expression analysis of DNAJC3-AS1 and microRNA-27b (miR-27b) at both mature and premature levels was determined by RT-qPCR. HCC patients were followed up for 5 years to analyze the prognostic value of DNAJC3-AS1 for HCC. The direct interaction between DNAJC3-AS1 and premature miR-27b was analyzed with RNA pull-down assay. Subcellular analysis of DNAJC3-AS1 was explored by subcellular fractionation assay. DNAJC3-AS1 overexpression and knockdown were carried out to analyze the role of DNAJC3-AS1 in miR-27b maturation. Cell proliferation was analyzed by BrdU assay.Results: DNAJC3-AS1 was overexpressed in HCC and predicts the poor survival. MiR-27b was downregulated at mature miRNA level, but upregulated at premature level. DNAJC3-AS1 directly interacted with premature miR-27b and was localized to both nuclear and cytoplasm. DNAJC3-AS1 overexpression upregulated premature miR-27b and downregulated mature miR-27b, while DNAJC3-AS1 knockdown led to the opposite results. DNAJC3-AS1 suppressed the role of miR-27b in inhibiting cell proliferation.Conclusion: DNAJC3-AS1 promotes HCC by sponging premature miR-27b and might be a biomarker and therapeutic target for HCC.Keywords: DNAJC3-AS1, miR-27b, hepatocellular carcinoma, maturationFu CLi JLi PCheng DDove Medical Pressarticlednajc3-as1mir-27bhepatocellular carcinomamaturationNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancer Management and Research, Vol Volume 13, Pp 8575-8583 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dnajc3-as1 mir-27b hepatocellular carcinoma maturation Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	dnajc3-as1 mir-27b hepatocellular carcinoma maturation Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Fu C Li J Li P Cheng D LncRNA DNAJC3-AS1 Promotes Hepatocellular Carcinoma (HCC) Progression via Sponging Premature miR-27b
description	Changbo Fu,1 Jianxiu Li,2 Ping Li,3 Dan Cheng4 1Department of Hepatobiliary and Pancreatic Surgery, Hubei Cancer Hospital, Wuhan City, 430000, People’s Republic of China; 2Disinfection Supply Center of Weifang Yidu Central Hospital, Weifang City, 266000, People’s Republic of China; 3Emergency Care Center of Qingdao Central Hospital, Qingdao City, 266000, People’s Republic of China; 4Department of Liver Disease Infection, Edong Healthcare City Hospital of Traditional Chinese Medicine, Infections Disease Hospital, Huangshi City, 435000, People’s Republic of ChinaCorrespondence: Dan ChengDepartment of Liver Disease Infection, Edong Healthcare City Hospital of Traditional Chinese Medicine, Infections Disease Hospital, No. 634 Shenxia Road, Huangshi City, 435000, People’s Republic of ChinaEmail chengdandisease@163.comPurpose: Long non-coding RNA (lncRNA) DNAJC3 antisense RNA 1 (head to head) (DNAJC3-AS1) plays a key role in the progression of several cancers. However, its biological role in hepatocellular carcinoma (HCC) is still unclear. We aimed to investigate the role of DNAJC3-AS1 in the development of HCC and reveal the potential mechanisms.Materials and Methods: Expression analysis of DNAJC3-AS1 and microRNA-27b (miR-27b) at both mature and premature levels was determined by RT-qPCR. HCC patients were followed up for 5 years to analyze the prognostic value of DNAJC3-AS1 for HCC. The direct interaction between DNAJC3-AS1 and premature miR-27b was analyzed with RNA pull-down assay. Subcellular analysis of DNAJC3-AS1 was explored by subcellular fractionation assay. DNAJC3-AS1 overexpression and knockdown were carried out to analyze the role of DNAJC3-AS1 in miR-27b maturation. Cell proliferation was analyzed by BrdU assay.Results: DNAJC3-AS1 was overexpressed in HCC and predicts the poor survival. MiR-27b was downregulated at mature miRNA level, but upregulated at premature level. DNAJC3-AS1 directly interacted with premature miR-27b and was localized to both nuclear and cytoplasm. DNAJC3-AS1 overexpression upregulated premature miR-27b and downregulated mature miR-27b, while DNAJC3-AS1 knockdown led to the opposite results. DNAJC3-AS1 suppressed the role of miR-27b in inhibiting cell proliferation.Conclusion: DNAJC3-AS1 promotes HCC by sponging premature miR-27b and might be a biomarker and therapeutic target for HCC.Keywords: DNAJC3-AS1, miR-27b, hepatocellular carcinoma, maturation
format	article
author	Fu C Li J Li P Cheng D
author_facet	Fu C Li J Li P Cheng D
author_sort	Fu C
title	LncRNA DNAJC3-AS1 Promotes Hepatocellular Carcinoma (HCC) Progression via Sponging Premature miR-27b
title_short	LncRNA DNAJC3-AS1 Promotes Hepatocellular Carcinoma (HCC) Progression via Sponging Premature miR-27b
title_full	LncRNA DNAJC3-AS1 Promotes Hepatocellular Carcinoma (HCC) Progression via Sponging Premature miR-27b
title_fullStr	LncRNA DNAJC3-AS1 Promotes Hepatocellular Carcinoma (HCC) Progression via Sponging Premature miR-27b
title_full_unstemmed	LncRNA DNAJC3-AS1 Promotes Hepatocellular Carcinoma (HCC) Progression via Sponging Premature miR-27b
title_sort	lncrna dnajc3-as1 promotes hepatocellular carcinoma (hcc) progression via sponging premature mir-27b
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/d503ff161e5947ceabcda6104e0b42cd
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LncRNA DNAJC3-AS1 Promotes Hepatocellular Carcinoma (HCC) Progression via Sponging Premature miR-27b

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