Modulation of Adhesion Molecules Expression by Different Metalloproteases Isolated from <i>Bothrops</i> Snakes

Snake venom metalloproteinases (SVMP) are involved in local inflammatory reactions observed after snakebites. Based on domain composition, they are classified as PI (pro-domain + proteolytic domain), PII (PI + disintegrin-like domains), or PIII (PII + cysteine-rich domains). Here, we studied the rol...

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Autores principales:	Bianca C. Zychar, Patrícia B. Clissa, Eneas Carvalho, Adilson S. Alves, Cristiani Baldo, Eliana L. Faquim-Mauro, Luís Roberto C. Gonçalves
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Bothrops</i> metalloproteases inflammation microcirculation adhesion molecules leukocyte-endothelium interactions Medicine R
Acceso en línea:	https://doaj.org/article/d50435301b2e4326903e1b6fdff1870f
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spelling	oai:doaj.org-article:d50435301b2e4326903e1b6fdff1870f2021-11-25T19:08:57ZModulation of Adhesion Molecules Expression by Different Metalloproteases Isolated from <i>Bothrops</i> Snakes10.3390/toxins131108032072-6651https://doaj.org/article/d50435301b2e4326903e1b6fdff1870f2021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6651/13/11/803https://doaj.org/toc/2072-6651Snake venom metalloproteinases (SVMP) are involved in local inflammatory reactions observed after snakebites. Based on domain composition, they are classified as PI (pro-domain + proteolytic domain), PII (PI + disintegrin-like domains), or PIII (PII + cysteine-rich domains). Here, we studied the role of different SVMPs domains in inducing the expression of adhesion molecules at the microcirculation of the cremaster muscle of mice. We used Jararhagin (Jar)—a PIII SVMP with intense hemorrhagic activity, and Jar-C—a Jar devoid of the catalytic domain, with no hemorrhagic activity, both isolated from <i>B. jararaca</i> venom and BnP-1—a weakly hemorrhagic P1 SVMP from <i>B. neuwiedi</i> venom. Toxins (0.5 µg) or PBS (100 µL) were injected into the scrotum of mice, and 2, 4, or 24 h later, the protein and gene expression of CD54 and CD31 in the endothelium, and integrins (CD11a and CD11b), expressed in leukocytes were evaluated. Toxins induced significant increases in CD54, CD11a, and CD11b at the initial time and a time-related increase in CD31 expression. In conclusion, our results suggest that, despite differences in hemorrhagic activities and domain composition of the SVMPs used in this study, they behave similarly to the induction of expression of adhesion molecules that promote leukocyte recruitment.Bianca C. ZycharPatrícia B. ClissaEneas CarvalhoAdilson S. AlvesCristiani BaldoEliana L. Faquim-MauroLuís Roberto C. GonçalvesMDPI AGarticle<i>Bothrops</i>metalloproteasesinflammationmicrocirculationadhesion moleculesleukocyte-endothelium interactionsMedicineRENToxins, Vol 13, Iss 803, p 803 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Bothrops</i> metalloproteases inflammation microcirculation adhesion molecules leukocyte-endothelium interactions Medicine R
spellingShingle	<i>Bothrops</i> metalloproteases inflammation microcirculation adhesion molecules leukocyte-endothelium interactions Medicine R Bianca C. Zychar Patrícia B. Clissa Eneas Carvalho Adilson S. Alves Cristiani Baldo Eliana L. Faquim-Mauro Luís Roberto C. Gonçalves Modulation of Adhesion Molecules Expression by Different Metalloproteases Isolated from <i>Bothrops</i> Snakes
description	Snake venom metalloproteinases (SVMP) are involved in local inflammatory reactions observed after snakebites. Based on domain composition, they are classified as PI (pro-domain + proteolytic domain), PII (PI + disintegrin-like domains), or PIII (PII + cysteine-rich domains). Here, we studied the role of different SVMPs domains in inducing the expression of adhesion molecules at the microcirculation of the cremaster muscle of mice. We used Jararhagin (Jar)—a PIII SVMP with intense hemorrhagic activity, and Jar-C—a Jar devoid of the catalytic domain, with no hemorrhagic activity, both isolated from <i>B. jararaca</i> venom and BnP-1—a weakly hemorrhagic P1 SVMP from <i>B. neuwiedi</i> venom. Toxins (0.5 µg) or PBS (100 µL) were injected into the scrotum of mice, and 2, 4, or 24 h later, the protein and gene expression of CD54 and CD31 in the endothelium, and integrins (CD11a and CD11b), expressed in leukocytes were evaluated. Toxins induced significant increases in CD54, CD11a, and CD11b at the initial time and a time-related increase in CD31 expression. In conclusion, our results suggest that, despite differences in hemorrhagic activities and domain composition of the SVMPs used in this study, they behave similarly to the induction of expression of adhesion molecules that promote leukocyte recruitment.
format	article
author	Bianca C. Zychar Patrícia B. Clissa Eneas Carvalho Adilson S. Alves Cristiani Baldo Eliana L. Faquim-Mauro Luís Roberto C. Gonçalves
author_facet	Bianca C. Zychar Patrícia B. Clissa Eneas Carvalho Adilson S. Alves Cristiani Baldo Eliana L. Faquim-Mauro Luís Roberto C. Gonçalves
author_sort	Bianca C. Zychar
title	Modulation of Adhesion Molecules Expression by Different Metalloproteases Isolated from <i>Bothrops</i> Snakes
title_short	Modulation of Adhesion Molecules Expression by Different Metalloproteases Isolated from <i>Bothrops</i> Snakes
title_full	Modulation of Adhesion Molecules Expression by Different Metalloproteases Isolated from <i>Bothrops</i> Snakes
title_fullStr	Modulation of Adhesion Molecules Expression by Different Metalloproteases Isolated from <i>Bothrops</i> Snakes
title_full_unstemmed	Modulation of Adhesion Molecules Expression by Different Metalloproteases Isolated from <i>Bothrops</i> Snakes
title_sort	modulation of adhesion molecules expression by different metalloproteases isolated from <i>bothrops</i> snakes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d50435301b2e4326903e1b6fdff1870f
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Modulation of Adhesion Molecules Expression by Different Metalloproteases Isolated from <i>Bothrops</i> Snakes

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