Anti-Platelet Fraction Isolated from Galega Officinalis
A fraction from crude extract of Galega officinalis has been purified by column chromatography on Sephadex G-25, Sepharose 4B, DEAE-Cellulose and Sephadex G-100. The final purification factor of the fraction is 120. The peak in elution profile after Sephadex G-150 shows a molecular weight of 100-140...
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| Format: | article |
| Langue: | EN |
| Publié: |
Sciendo
2016
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| Accès en ligne: | https://doaj.org/article/d50496822f2a4cf3b1bc83a9cbc624f1 |
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| Résumé: | A fraction from crude extract of Galega officinalis has been purified by column chromatography on Sephadex G-25, Sepharose 4B, DEAE-Cellulose and Sephadex G-100. The final purification factor of the fraction is 120. The peak in elution profile after Sephadex G-150 shows a molecular weight of 100-140 kDa. The isolated fraction appears to have 74% polysaccharides and 23% of proteins. No loss of activity of the final fraction is observed after storage for several months at 4°C and in lyophilized condition. The fraction compounds inhibit platelet aggregation induced by ADP, collagen and thrombin. |
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