Printable graphene BioFETs for DNA quantification in Lab-on-PCB microsystems

Abstract Lab-on-Chip is a technology that aims to transform the Point-of-Care (PoC) diagnostics field; nonetheless a commercial production compatible technology is yet to be established. Lab-on-Printed Circuit Board (Lab-on-PCB) is currently considered as a promising candidate technology for cost-aw...

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Autores principales:	Sotirios Papamatthaiou, Pedro Estrela, Despina Moschou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d50c6cea50b7493a8ea9dd1da7c01e9d
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spelling	oai:doaj.org-article:d50c6cea50b7493a8ea9dd1da7c01e9d2021-12-02T17:02:13ZPrintable graphene BioFETs for DNA quantification in Lab-on-PCB microsystems10.1038/s41598-021-89367-12045-2322https://doaj.org/article/d50c6cea50b7493a8ea9dd1da7c01e9d2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89367-1https://doaj.org/toc/2045-2322Abstract Lab-on-Chip is a technology that aims to transform the Point-of-Care (PoC) diagnostics field; nonetheless a commercial production compatible technology is yet to be established. Lab-on-Printed Circuit Board (Lab-on-PCB) is currently considered as a promising candidate technology for cost-aware but simultaneously high specification applications, requiring multi-component microsystem implementations, due to its inherent compatibility with electronics and the long-standing industrial manufacturing basis. In this work, we demonstrate the first electrolyte gated field-effect transistor (FET) DNA biosensor implemented on commercially fabricated PCB in a planar layout. Graphene ink was drop-casted to form the transistor channel and PNA probes were immobilized on the graphene channel, enabling label-free DNA detection. It is shown that the sensor can selectively detect the complementary DNA sequence, following a fully inkjet-printing compatible manufacturing process. The results demonstrate the potential for the effortless integration of FET sensors into Lab-on-PCB diagnostic platforms, paving the way for even higher sensitivity quantification than the current Lab-on-PCB state-of-the-art of passive electrode electrochemical sensing. The substitution of such biosensors with our presented FET structures, promises further reduction of the time-to-result in microsystems combining sequential DNA amplification and detection modules to few minutes, since much fewer amplification cycles are required even for low-abundance nucleic acid targets.Sotirios PapamatthaiouPedro EstrelaDespina MoschouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sotirios Papamatthaiou Pedro Estrela Despina Moschou Printable graphene BioFETs for DNA quantification in Lab-on-PCB microsystems
description	Abstract Lab-on-Chip is a technology that aims to transform the Point-of-Care (PoC) diagnostics field; nonetheless a commercial production compatible technology is yet to be established. Lab-on-Printed Circuit Board (Lab-on-PCB) is currently considered as a promising candidate technology for cost-aware but simultaneously high specification applications, requiring multi-component microsystem implementations, due to its inherent compatibility with electronics and the long-standing industrial manufacturing basis. In this work, we demonstrate the first electrolyte gated field-effect transistor (FET) DNA biosensor implemented on commercially fabricated PCB in a planar layout. Graphene ink was drop-casted to form the transistor channel and PNA probes were immobilized on the graphene channel, enabling label-free DNA detection. It is shown that the sensor can selectively detect the complementary DNA sequence, following a fully inkjet-printing compatible manufacturing process. The results demonstrate the potential for the effortless integration of FET sensors into Lab-on-PCB diagnostic platforms, paving the way for even higher sensitivity quantification than the current Lab-on-PCB state-of-the-art of passive electrode electrochemical sensing. The substitution of such biosensors with our presented FET structures, promises further reduction of the time-to-result in microsystems combining sequential DNA amplification and detection modules to few minutes, since much fewer amplification cycles are required even for low-abundance nucleic acid targets.
format	article
author	Sotirios Papamatthaiou Pedro Estrela Despina Moschou
author_facet	Sotirios Papamatthaiou Pedro Estrela Despina Moschou
author_sort	Sotirios Papamatthaiou
title	Printable graphene BioFETs for DNA quantification in Lab-on-PCB microsystems
title_short	Printable graphene BioFETs for DNA quantification in Lab-on-PCB microsystems
title_full	Printable graphene BioFETs for DNA quantification in Lab-on-PCB microsystems
title_fullStr	Printable graphene BioFETs for DNA quantification in Lab-on-PCB microsystems
title_full_unstemmed	Printable graphene BioFETs for DNA quantification in Lab-on-PCB microsystems
title_sort	printable graphene biofets for dna quantification in lab-on-pcb microsystems
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d50c6cea50b7493a8ea9dd1da7c01e9d
work_keys_str_mv	AT sotiriospapamatthaiou printablegraphenebiofetsfordnaquantificationinlabonpcbmicrosystems AT pedroestrela printablegraphenebiofetsfordnaquantificationinlabonpcbmicrosystems AT despinamoschou printablegraphenebiofetsfordnaquantificationinlabonpcbmicrosystems
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Printable graphene BioFETs for DNA quantification in Lab-on-PCB microsystems

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