An innovative cell-laden α-TCP/collagen scaffold fabricated using a two-step printing process for potential application in regenerating hard tissues

Abstract Cell-laden scaffolds are widely investigated in tissue engineering because they can provide homogenous cell distribution after long culture periods, and deposit multiple types of cells into a designed region. However, producing a bioceramic 3D cell-laden scaffold is difficult because of the...

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Autores principales:	Won Jin Kim, Hui-Suk Yun, Geun Hyung Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d512ce7ba2ec4515b689894901bc57e3
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spelling	oai:doaj.org-article:d512ce7ba2ec4515b689894901bc57e32021-12-02T12:30:27ZAn innovative cell-laden α-TCP/collagen scaffold fabricated using a two-step printing process for potential application in regenerating hard tissues10.1038/s41598-017-03455-92045-2322https://doaj.org/article/d512ce7ba2ec4515b689894901bc57e32017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03455-9https://doaj.org/toc/2045-2322Abstract Cell-laden scaffolds are widely investigated in tissue engineering because they can provide homogenous cell distribution after long culture periods, and deposit multiple types of cells into a designed region. However, producing a bioceramic 3D cell-laden scaffold is difficult because of the low processability of cell-loaded bioceramics. Therefore, designing a 3D bioceramic cell-laden scaffold is important for ceramic-based tissue regeneration. Here, we propose a new strategy to fabricate an alpha-tricalcium-phosphate (α-TCP)/collagen cell-laden scaffold, using preosteoblasts (MC3T3-E1), in which the volume fraction of the ceramic exceeded 70% and was fabricated using a two-step printing process. To fabricate a multi-layered cell-laden scaffold, we manipulated processing parameters, such as the diameter of the printing nozzle, pneumatic pressure, and volume fraction of α-TCP, to attain a stable processing region. A cell-laden pure collagen scaffold and an α-TCP/collagen scaffold loaded with cells via a simple dipping method were used as controls. Their pore geometry was similar to that of the experimental scaffold. Physical properties and bioactivities showed that the designed scaffold demonstrated significantly higher cellular activities, including metabolic activity and mineralization, compared with those of the controls. Our results indicate that the proposed cell-laden ceramic scaffold can potentially be used for bone regeneration.Won Jin KimHui-Suk YunGeun Hyung KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Won Jin Kim Hui-Suk Yun Geun Hyung Kim An innovative cell-laden α-TCP/collagen scaffold fabricated using a two-step printing process for potential application in regenerating hard tissues
description	Abstract Cell-laden scaffolds are widely investigated in tissue engineering because they can provide homogenous cell distribution after long culture periods, and deposit multiple types of cells into a designed region. However, producing a bioceramic 3D cell-laden scaffold is difficult because of the low processability of cell-loaded bioceramics. Therefore, designing a 3D bioceramic cell-laden scaffold is important for ceramic-based tissue regeneration. Here, we propose a new strategy to fabricate an alpha-tricalcium-phosphate (α-TCP)/collagen cell-laden scaffold, using preosteoblasts (MC3T3-E1), in which the volume fraction of the ceramic exceeded 70% and was fabricated using a two-step printing process. To fabricate a multi-layered cell-laden scaffold, we manipulated processing parameters, such as the diameter of the printing nozzle, pneumatic pressure, and volume fraction of α-TCP, to attain a stable processing region. A cell-laden pure collagen scaffold and an α-TCP/collagen scaffold loaded with cells via a simple dipping method were used as controls. Their pore geometry was similar to that of the experimental scaffold. Physical properties and bioactivities showed that the designed scaffold demonstrated significantly higher cellular activities, including metabolic activity and mineralization, compared with those of the controls. Our results indicate that the proposed cell-laden ceramic scaffold can potentially be used for bone regeneration.
format	article
author	Won Jin Kim Hui-Suk Yun Geun Hyung Kim
author_facet	Won Jin Kim Hui-Suk Yun Geun Hyung Kim
author_sort	Won Jin Kim
title	An innovative cell-laden α-TCP/collagen scaffold fabricated using a two-step printing process for potential application in regenerating hard tissues
title_short	An innovative cell-laden α-TCP/collagen scaffold fabricated using a two-step printing process for potential application in regenerating hard tissues
title_full	An innovative cell-laden α-TCP/collagen scaffold fabricated using a two-step printing process for potential application in regenerating hard tissues
title_fullStr	An innovative cell-laden α-TCP/collagen scaffold fabricated using a two-step printing process for potential application in regenerating hard tissues
title_full_unstemmed	An innovative cell-laden α-TCP/collagen scaffold fabricated using a two-step printing process for potential application in regenerating hard tissues
title_sort	innovative cell-laden α-tcp/collagen scaffold fabricated using a two-step printing process for potential application in regenerating hard tissues
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d512ce7ba2ec4515b689894901bc57e3
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An innovative cell-laden α-TCP/collagen scaffold fabricated using a two-step printing process for potential application in regenerating hard tissues

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