Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM

Combining cryo-electron tomography with subtomogram averaging (StA) allows the in situ structure determination of proteins and protein complexes. Here, the authors present the hybrid StA (hStA) workflow that combines the advantages of single particle cryo-EM and StA and consists of a tomographic dat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ricardo M. Sanchez, Yingyi Zhang, Wenbo Chen, Lea Dietrich, Mikhail Kudryashev
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/d517dfd8abf9429a9e657378002f8d85
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d517dfd8abf9429a9e657378002f8d85
record_format	dspace
spelling	oai:doaj.org-article:d517dfd8abf9429a9e657378002f8d852021-12-02T16:43:46ZSubnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM10.1038/s41467-020-17466-02041-1723https://doaj.org/article/d517dfd8abf9429a9e657378002f8d852020-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17466-0https://doaj.org/toc/2041-1723Combining cryo-electron tomography with subtomogram averaging (StA) allows the in situ structure determination of proteins and protein complexes. Here, the authors present the hybrid StA (hStA) workflow that combines the advantages of single particle cryo-EM and StA and consists of a tomographic data collection scheme and a data processing workflow and they demonstrate how hStA can improve the resolution using two examples: the ion channel RyR1 and tobacco mosaic virus.Ricardo M. SanchezYingyi ZhangWenbo ChenLea DietrichMikhail KudryashevNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Ricardo M. Sanchez Yingyi Zhang Wenbo Chen Lea Dietrich Mikhail Kudryashev Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM
description	Combining cryo-electron tomography with subtomogram averaging (StA) allows the in situ structure determination of proteins and protein complexes. Here, the authors present the hybrid StA (hStA) workflow that combines the advantages of single particle cryo-EM and StA and consists of a tomographic data collection scheme and a data processing workflow and they demonstrate how hStA can improve the resolution using two examples: the ion channel RyR1 and tobacco mosaic virus.
format	article
author	Ricardo M. Sanchez Yingyi Zhang Wenbo Chen Lea Dietrich Mikhail Kudryashev
author_facet	Ricardo M. Sanchez Yingyi Zhang Wenbo Chen Lea Dietrich Mikhail Kudryashev
author_sort	Ricardo M. Sanchez
title	Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM
title_short	Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM
title_full	Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM
title_fullStr	Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM
title_full_unstemmed	Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM
title_sort	subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-em
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/d517dfd8abf9429a9e657378002f8d85
work_keys_str_mv	AT ricardomsanchez subnanometerresolutionstructuredeterminationinsitubyhybridsubtomogramaveragingsingleparticlecryoem AT yingyizhang subnanometerresolutionstructuredeterminationinsitubyhybridsubtomogramaveragingsingleparticlecryoem AT wenbochen subnanometerresolutionstructuredeterminationinsitubyhybridsubtomogramaveragingsingleparticlecryoem AT leadietrich subnanometerresolutionstructuredeterminationinsitubyhybridsubtomogramaveragingsingleparticlecryoem AT mikhailkudryashev subnanometerresolutionstructuredeterminationinsitubyhybridsubtomogramaveragingsingleparticlecryoem
_version_	1718383541939601408

Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM

Ejemplares similares