Determination of amphetamines, ketamine and their metabolites in hair with high-speed grinding and solid-phase microextraction followed by LC-MS

A novel hair sample pre-treatment method based on high-speed grinding and solid-phase microextraction (SPME) had been applied for the determination of amphetamines, ketamine and their metabolites in hair samples by liquid chromatography-mass spectrometry (LC-MS). A 20 mg sample of hair was ground wi...

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Autores principales:	Liang Meng, Yong Dai, Chen Chen, Jun Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	forensic sciences forensic toxicology drugs of abuse high-speed grinding hair analysis liquid chromatography-mass spectrometry solid-phase microextraction Criminal law and procedure K5000-5582 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/d51b7564dffc4d73ba71eb9a8ec24e86
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spelling	oai:doaj.org-article:d51b7564dffc4d73ba71eb9a8ec24e862021-12-01T14:40:59ZDetermination of amphetamines, ketamine and their metabolites in hair with high-speed grinding and solid-phase microextraction followed by LC-MS2096-17902471-141110.1080/20961790.2020.1838403https://doaj.org/article/d51b7564dffc4d73ba71eb9a8ec24e862021-07-01T00:00:00Zhttp://dx.doi.org/10.1080/20961790.2020.1838403https://doaj.org/toc/2096-1790https://doaj.org/toc/2471-1411A novel hair sample pre-treatment method based on high-speed grinding and solid-phase microextraction (SPME) had been applied for the determination of amphetamines, ketamine and their metabolites in hair samples by liquid chromatography-mass spectrometry (LC-MS). A 20 mg sample of hair was ground with 2 mL of saturated sodium carbonate solution using a high-efficiency hair grinder with 70 Hz oscillation for 2 min at 4 °C. After centrifuging, 1.5 mL of the supernatant was transferred and treated with SPME by direct immersion (DI-SPME). The target analytes extracted by fibre were desorbed and analysed using LC-MS. Under the optimum conditions, a recovery of 90.2%–95.8% was obtained for all analytes. The analytical method was linear for all analytes in the range from 0.2 to 10 ng/mg with the correlation coefficient ranging from 0.9985 to 0.9993. The detection limits for all analytes were estimated to be 0.067 ng/mg. The accuracy (mean relative error) was within ±6.9% and the precision (relative standard error) was less than 6.8%. The combination of high-speed grinding of hair and SPME had the advantages of being easy to perform, environment-friendly and high in detection sensitivity. The proposed method offered an alternative analytical approach for the sensitive detection of drugs in hair samples for forensic purposes.Key Points The SPME was involved for the determination of drugs in hair with LC-MS. The hair high-speed grinding combined with SPME was firstly developed. Good linearity, sensitivity, recovery and precision were achieved.Liang MengYong DaiChen ChenJun ZhangTaylor & Francis Grouparticleforensic sciencesforensic toxicologydrugs of abusehigh-speed grindinghair analysisliquid chromatography-mass spectrometrysolid-phase microextractionCriminal law and procedureK5000-5582Public aspects of medicineRA1-1270ENForensic Sciences Research, Vol 6, Iss 3, Pp 273-280 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	forensic sciences forensic toxicology drugs of abuse high-speed grinding hair analysis liquid chromatography-mass spectrometry solid-phase microextraction Criminal law and procedure K5000-5582 Public aspects of medicine RA1-1270
spellingShingle	forensic sciences forensic toxicology drugs of abuse high-speed grinding hair analysis liquid chromatography-mass spectrometry solid-phase microextraction Criminal law and procedure K5000-5582 Public aspects of medicine RA1-1270 Liang Meng Yong Dai Chen Chen Jun Zhang Determination of amphetamines, ketamine and their metabolites in hair with high-speed grinding and solid-phase microextraction followed by LC-MS
description	A novel hair sample pre-treatment method based on high-speed grinding and solid-phase microextraction (SPME) had been applied for the determination of amphetamines, ketamine and their metabolites in hair samples by liquid chromatography-mass spectrometry (LC-MS). A 20 mg sample of hair was ground with 2 mL of saturated sodium carbonate solution using a high-efficiency hair grinder with 70 Hz oscillation for 2 min at 4 °C. After centrifuging, 1.5 mL of the supernatant was transferred and treated with SPME by direct immersion (DI-SPME). The target analytes extracted by fibre were desorbed and analysed using LC-MS. Under the optimum conditions, a recovery of 90.2%–95.8% was obtained for all analytes. The analytical method was linear for all analytes in the range from 0.2 to 10 ng/mg with the correlation coefficient ranging from 0.9985 to 0.9993. The detection limits for all analytes were estimated to be 0.067 ng/mg. The accuracy (mean relative error) was within ±6.9% and the precision (relative standard error) was less than 6.8%. The combination of high-speed grinding of hair and SPME had the advantages of being easy to perform, environment-friendly and high in detection sensitivity. The proposed method offered an alternative analytical approach for the sensitive detection of drugs in hair samples for forensic purposes.Key Points The SPME was involved for the determination of drugs in hair with LC-MS. The hair high-speed grinding combined with SPME was firstly developed. Good linearity, sensitivity, recovery and precision were achieved.
format	article
author	Liang Meng Yong Dai Chen Chen Jun Zhang
author_facet	Liang Meng Yong Dai Chen Chen Jun Zhang
author_sort	Liang Meng
title	Determination of amphetamines, ketamine and their metabolites in hair with high-speed grinding and solid-phase microextraction followed by LC-MS
title_short	Determination of amphetamines, ketamine and their metabolites in hair with high-speed grinding and solid-phase microextraction followed by LC-MS
title_full	Determination of amphetamines, ketamine and their metabolites in hair with high-speed grinding and solid-phase microextraction followed by LC-MS
title_fullStr	Determination of amphetamines, ketamine and their metabolites in hair with high-speed grinding and solid-phase microextraction followed by LC-MS
title_full_unstemmed	Determination of amphetamines, ketamine and their metabolites in hair with high-speed grinding and solid-phase microextraction followed by LC-MS
title_sort	determination of amphetamines, ketamine and their metabolites in hair with high-speed grinding and solid-phase microextraction followed by lc-ms
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/d51b7564dffc4d73ba71eb9a8ec24e86
work_keys_str_mv	AT liangmeng determinationofamphetaminesketamineandtheirmetabolitesinhairwithhighspeedgrindingandsolidphasemicroextractionfollowedbylcms AT yongdai determinationofamphetaminesketamineandtheirmetabolitesinhairwithhighspeedgrindingandsolidphasemicroextractionfollowedbylcms AT chenchen determinationofamphetaminesketamineandtheirmetabolitesinhairwithhighspeedgrindingandsolidphasemicroextractionfollowedbylcms AT junzhang determinationofamphetaminesketamineandtheirmetabolitesinhairwithhighspeedgrindingandsolidphasemicroextractionfollowedbylcms
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Determination of amphetamines, ketamine and their metabolites in hair with high-speed grinding and solid-phase microextraction followed by LC-MS

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