Holocene life and microbiome profiling in ancient tropical Lake Chalco, Mexico

Abstract Metagenomic and traditional paleolimnological approaches are suitable to infer past biological and environmental changes, however, they are often applied independently, especially in tropical regions. We combined both approaches to investigate Holocene Prokaryote and Eukaryote diversity and...

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Autores principales: Bárbara Moguel, Liseth Pérez, Luis D. Alcaraz, Jazmín Blaz, Margarita Caballero, Israel Muñoz-Velasco, Arturo Becerra, Juan P. Laclette, Beatriz Ortega-Guerrero, Claudia S. Romero-Oliva, Luis Herrera-Estrella, Socorro Lozano-García
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/d52295b99e1b4a91974cc15243ad056f
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spelling oai:doaj.org-article:d52295b99e1b4a91974cc15243ad056f2021-12-02T15:22:56ZHolocene life and microbiome profiling in ancient tropical Lake Chalco, Mexico10.1038/s41598-021-92981-82045-2322https://doaj.org/article/d52295b99e1b4a91974cc15243ad056f2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92981-8https://doaj.org/toc/2045-2322Abstract Metagenomic and traditional paleolimnological approaches are suitable to infer past biological and environmental changes, however, they are often applied independently, especially in tropical regions. We combined both approaches to investigate Holocene Prokaryote and Eukaryote diversity and microbial metabolic pathways in ancient Lake Chalco, Mexico. Here, we report on diversity among a large number of lineages (36,722 OTUs) and functional diversity (27,636,243 non-clustered predicted proteins, and 6,144 annotated protein-family genes). The most abundant domain is Bacteria (81%), followed by Archaea (15%) and Eukarya (3%). We also determined the diversity of protein families and their relationship to metabolic pathways. The early Holocene (> 11,000 cal years BP) lake was characterized by cool, freshwater conditions, which later became warmer and hyposaline (11,000–6,000 cal years BP). We found high abundances of cyanobacteria, and fungi groups associated with mature forests in these sediments. Bacteria and Archaea include mainly anaerobes and extremophiles that are involved in the sulfur, nitrogen, and carbon cycles. We found evidence for early human impacts, including landscape modifications and lake eutrophication, which began ~ 6,000 cal years BP. Subsaline, temperate conditions were inferred for the past 5,000 years. Finally, we found nitrogen-fixing bacteria and protein-family genes that are linked to contaminated environments, as well as several fungal pathogens of crops in near-surface sediments.Bárbara MoguelLiseth PérezLuis D. AlcarazJazmín BlazMargarita CaballeroIsrael Muñoz-VelascoArturo BecerraJuan P. LacletteBeatriz Ortega-GuerreroClaudia S. Romero-OlivaLuis Herrera-EstrellaSocorro Lozano-GarcíaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Bárbara Moguel
Liseth Pérez
Luis D. Alcaraz
Jazmín Blaz
Margarita Caballero
Israel Muñoz-Velasco
Arturo Becerra
Juan P. Laclette
Beatriz Ortega-Guerrero
Claudia S. Romero-Oliva
Luis Herrera-Estrella
Socorro Lozano-García
Holocene life and microbiome profiling in ancient tropical Lake Chalco, Mexico
description Abstract Metagenomic and traditional paleolimnological approaches are suitable to infer past biological and environmental changes, however, they are often applied independently, especially in tropical regions. We combined both approaches to investigate Holocene Prokaryote and Eukaryote diversity and microbial metabolic pathways in ancient Lake Chalco, Mexico. Here, we report on diversity among a large number of lineages (36,722 OTUs) and functional diversity (27,636,243 non-clustered predicted proteins, and 6,144 annotated protein-family genes). The most abundant domain is Bacteria (81%), followed by Archaea (15%) and Eukarya (3%). We also determined the diversity of protein families and their relationship to metabolic pathways. The early Holocene (> 11,000 cal years BP) lake was characterized by cool, freshwater conditions, which later became warmer and hyposaline (11,000–6,000 cal years BP). We found high abundances of cyanobacteria, and fungi groups associated with mature forests in these sediments. Bacteria and Archaea include mainly anaerobes and extremophiles that are involved in the sulfur, nitrogen, and carbon cycles. We found evidence for early human impacts, including landscape modifications and lake eutrophication, which began ~ 6,000 cal years BP. Subsaline, temperate conditions were inferred for the past 5,000 years. Finally, we found nitrogen-fixing bacteria and protein-family genes that are linked to contaminated environments, as well as several fungal pathogens of crops in near-surface sediments.
format article
author Bárbara Moguel
Liseth Pérez
Luis D. Alcaraz
Jazmín Blaz
Margarita Caballero
Israel Muñoz-Velasco
Arturo Becerra
Juan P. Laclette
Beatriz Ortega-Guerrero
Claudia S. Romero-Oliva
Luis Herrera-Estrella
Socorro Lozano-García
author_facet Bárbara Moguel
Liseth Pérez
Luis D. Alcaraz
Jazmín Blaz
Margarita Caballero
Israel Muñoz-Velasco
Arturo Becerra
Juan P. Laclette
Beatriz Ortega-Guerrero
Claudia S. Romero-Oliva
Luis Herrera-Estrella
Socorro Lozano-García
author_sort Bárbara Moguel
title Holocene life and microbiome profiling in ancient tropical Lake Chalco, Mexico
title_short Holocene life and microbiome profiling in ancient tropical Lake Chalco, Mexico
title_full Holocene life and microbiome profiling in ancient tropical Lake Chalco, Mexico
title_fullStr Holocene life and microbiome profiling in ancient tropical Lake Chalco, Mexico
title_full_unstemmed Holocene life and microbiome profiling in ancient tropical Lake Chalco, Mexico
title_sort holocene life and microbiome profiling in ancient tropical lake chalco, mexico
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/d52295b99e1b4a91974cc15243ad056f
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