Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic Archaea

Activity-based protein profiling (ABPP) is a chemical proteomics method to profile activity states of enzymes under physiological conditions. Here the authors show that ABPP can be applied to archaeal serine hydrolases in the model organismSulfolobus acidocaldariusand can be used to identify novel p...

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Autores principales:	Susanne Zweerink, Verena Kallnik, Sabrina Ninck, Sabrina Nickel, Julia Verheyen, Marcel Blum, Alexander Wagner, Ingo Feldmann, Albert Sickmann, Sonja-Verena Albers, Christopher Bräsen, Farnusch Kaschani, Bettina Siebers, Markus Kaiser
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/d528a9e441ee4ec1bd18d1a9ea08e735
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spelling	oai:doaj.org-article:d528a9e441ee4ec1bd18d1a9ea08e7352021-12-02T17:01:18ZActivity-based protein profiling as a robust method for enzyme identification and screening in extremophilic Archaea10.1038/ncomms153522041-1723https://doaj.org/article/d528a9e441ee4ec1bd18d1a9ea08e7352017-05-01T00:00:00Zhttps://doi.org/10.1038/ncomms15352https://doaj.org/toc/2041-1723Activity-based protein profiling (ABPP) is a chemical proteomics method to profile activity states of enzymes under physiological conditions. Here the authors show that ABPP can be applied to archaeal serine hydrolases in the model organismSulfolobus acidocaldariusand can be used to identify novel putative serine hydrolases.Susanne ZweerinkVerena KallnikSabrina NinckSabrina NickelJulia VerheyenMarcel BlumAlexander WagnerIngo FeldmannAlbert SickmannSonja-Verena AlbersChristopher BräsenFarnusch KaschaniBettina SiebersMarkus KaiserNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Susanne Zweerink Verena Kallnik Sabrina Ninck Sabrina Nickel Julia Verheyen Marcel Blum Alexander Wagner Ingo Feldmann Albert Sickmann Sonja-Verena Albers Christopher Bräsen Farnusch Kaschani Bettina Siebers Markus Kaiser Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic Archaea
description	Activity-based protein profiling (ABPP) is a chemical proteomics method to profile activity states of enzymes under physiological conditions. Here the authors show that ABPP can be applied to archaeal serine hydrolases in the model organismSulfolobus acidocaldariusand can be used to identify novel putative serine hydrolases.
format	article
author	Susanne Zweerink Verena Kallnik Sabrina Ninck Sabrina Nickel Julia Verheyen Marcel Blum Alexander Wagner Ingo Feldmann Albert Sickmann Sonja-Verena Albers Christopher Bräsen Farnusch Kaschani Bettina Siebers Markus Kaiser
author_facet	Susanne Zweerink Verena Kallnik Sabrina Ninck Sabrina Nickel Julia Verheyen Marcel Blum Alexander Wagner Ingo Feldmann Albert Sickmann Sonja-Verena Albers Christopher Bräsen Farnusch Kaschani Bettina Siebers Markus Kaiser
author_sort	Susanne Zweerink
title	Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic Archaea
title_short	Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic Archaea
title_full	Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic Archaea
title_fullStr	Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic Archaea
title_full_unstemmed	Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic Archaea
title_sort	activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic archaea
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d528a9e441ee4ec1bd18d1a9ea08e735
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Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic Archaea

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