An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila

Drosophila transgenic RNAi can have drawbacks such as false positives and negative results. Here the authors develop a next generation RNAi system with reduced leakiness of expression and simultaneous knockdown.

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Autores principales: Huan-Huan Qiao, Fang Wang, Rong-Gang Xu, Jin Sun, Ruibao Zhu, Decai Mao, Xingjie Ren, Xia Wang, Yu Jia, Ping Peng, Da Shen, Lu-Ping Liu, Zhijie Chang, Guirong Wang, Shao Li, Jun-Yuan Ji, Qingfei Liu, Jian-Quan Ni
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/d52fc41852e74a78b2ee0fec8db480d4
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spelling oai:doaj.org-article:d52fc41852e74a78b2ee0fec8db480d42021-12-02T15:33:58ZAn efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila10.1038/s41467-018-06537-y2041-1723https://doaj.org/article/d52fc41852e74a78b2ee0fec8db480d42018-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-06537-yhttps://doaj.org/toc/2041-1723Drosophila transgenic RNAi can have drawbacks such as false positives and negative results. Here the authors develop a next generation RNAi system with reduced leakiness of expression and simultaneous knockdown.Huan-Huan QiaoFang WangRong-Gang XuJin SunRuibao ZhuDecai MaoXingjie RenXia WangYu JiaPing PengDa ShenLu-Ping LiuZhijie ChangGuirong WangShao LiJun-Yuan JiQingfei LiuJian-Quan NiNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-13 (2018)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Huan-Huan Qiao
Fang Wang
Rong-Gang Xu
Jin Sun
Ruibao Zhu
Decai Mao
Xingjie Ren
Xia Wang
Yu Jia
Ping Peng
Da Shen
Lu-Ping Liu
Zhijie Chang
Guirong Wang
Shao Li
Jun-Yuan Ji
Qingfei Liu
Jian-Quan Ni
An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila
description Drosophila transgenic RNAi can have drawbacks such as false positives and negative results. Here the authors develop a next generation RNAi system with reduced leakiness of expression and simultaneous knockdown.
format article
author Huan-Huan Qiao
Fang Wang
Rong-Gang Xu
Jin Sun
Ruibao Zhu
Decai Mao
Xingjie Ren
Xia Wang
Yu Jia
Ping Peng
Da Shen
Lu-Ping Liu
Zhijie Chang
Guirong Wang
Shao Li
Jun-Yuan Ji
Qingfei Liu
Jian-Quan Ni
author_facet Huan-Huan Qiao
Fang Wang
Rong-Gang Xu
Jin Sun
Ruibao Zhu
Decai Mao
Xingjie Ren
Xia Wang
Yu Jia
Ping Peng
Da Shen
Lu-Ping Liu
Zhijie Chang
Guirong Wang
Shao Li
Jun-Yuan Ji
Qingfei Liu
Jian-Quan Ni
author_sort Huan-Huan Qiao
title An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila
title_short An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila
title_full An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila
title_fullStr An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila
title_full_unstemmed An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila
title_sort efficient and multiple target transgenic rnai technique with low toxicity in drosophila
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/d52fc41852e74a78b2ee0fec8db480d4
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