Quantitative detection of human- and canine-associated Bacteroides genetic markers from an urban coastal lagoon

The contamination of water catchments by nonpoint source faecal pollution is a major issue affecting the microbial quality of receiving waters and is associated with the occurrence of a range of enteric illnesses in humans. The potential sources of faecal pollution in surface waters are diverse, inc...

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Autores principales:	Serhat A. Yasar, Toby J. T. Mills, Zehra I. Uluturk, Jason M. S. Ruszczyk, Rebecca J. LeBard, Brett A. Neilan
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	bacteroides faecal indicator bacteria microbial source tracking qpcr urban surface water Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/d5300cd1639e434e98f59e90c2ec9662
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spelling	oai:doaj.org-article:d5300cd1639e434e98f59e90c2ec96622021-11-06T11:24:25ZQuantitative detection of human- and canine-associated Bacteroides genetic markers from an urban coastal lagoon0273-12231996-973210.2166/wst.2021.341https://doaj.org/article/d5300cd1639e434e98f59e90c2ec96622021-10-01T00:00:00Zhttp://wst.iwaponline.com/content/84/7/1732https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732The contamination of water catchments by nonpoint source faecal pollution is a major issue affecting the microbial quality of receiving waters and is associated with the occurrence of a range of enteric illnesses in humans. The potential sources of faecal pollution in surface waters are diverse, including urban sewage leaks, surface runoff and wildlife contamination originating from a range of hosts. The major contributing hosts require identification to allow targeted management of this public health concern. In this study, two high-performing Microbial Source Tracking (MST) assays, HF183/Bac242 and BacCan-UCDmodif, were used for their ability to detect host-specific Bacteroides 16Sr RNA markers for faecal pollution in a 12-month study on an urban coastal lagoon in Sydney, Australia. The lagoon was found to contain year-round high numbers of human and canine faecal markers, as well as faecal indicator bacteria counts, suggesting considerable human and animal faecal pollution. The high sensitivity and specificity of the HF183/Bac242 and BacCan-UCDmodif assays, together with the manageable levels of PCR inhibition and high level DNA extraction efficiency obtained from lagoon water samples make these markers candidates for inclusion in an MST ‘toolbox’ for investigating host origins of faecal pollution in urban surface waters. HIGHLIGHTS This long-term study (twelve months with bi-monthly sampling) validated the use of HF183/BacCan242 and BacCan-UCDmodif to quantify host-associated faecal inputs in urban surface waters.; Correlations between MST markers, traditional FIB measurements, and physiochemical parameters indicate that the latter may be used to predict the likelihood of human- and canine-associated faecal inputs at this site.;Serhat A. YasarToby J. T. MillsZehra I. UluturkJason M. S. RuszczykRebecca J. LeBardBrett A. NeilanIWA Publishingarticlebacteroidesfaecal indicator bacteriamicrobial source trackingqpcrurban surface waterEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 84, Iss 7, Pp 1732-1744 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteroides faecal indicator bacteria microbial source tracking qpcr urban surface water Environmental technology. Sanitary engineering TD1-1066
spellingShingle	bacteroides faecal indicator bacteria microbial source tracking qpcr urban surface water Environmental technology. Sanitary engineering TD1-1066 Serhat A. Yasar Toby J. T. Mills Zehra I. Uluturk Jason M. S. Ruszczyk Rebecca J. LeBard Brett A. Neilan Quantitative detection of human- and canine-associated Bacteroides genetic markers from an urban coastal lagoon
description	The contamination of water catchments by nonpoint source faecal pollution is a major issue affecting the microbial quality of receiving waters and is associated with the occurrence of a range of enteric illnesses in humans. The potential sources of faecal pollution in surface waters are diverse, including urban sewage leaks, surface runoff and wildlife contamination originating from a range of hosts. The major contributing hosts require identification to allow targeted management of this public health concern. In this study, two high-performing Microbial Source Tracking (MST) assays, HF183/Bac242 and BacCan-UCDmodif, were used for their ability to detect host-specific Bacteroides 16Sr RNA markers for faecal pollution in a 12-month study on an urban coastal lagoon in Sydney, Australia. The lagoon was found to contain year-round high numbers of human and canine faecal markers, as well as faecal indicator bacteria counts, suggesting considerable human and animal faecal pollution. The high sensitivity and specificity of the HF183/Bac242 and BacCan-UCDmodif assays, together with the manageable levels of PCR inhibition and high level DNA extraction efficiency obtained from lagoon water samples make these markers candidates for inclusion in an MST ‘toolbox’ for investigating host origins of faecal pollution in urban surface waters. HIGHLIGHTS This long-term study (twelve months with bi-monthly sampling) validated the use of HF183/BacCan242 and BacCan-UCDmodif to quantify host-associated faecal inputs in urban surface waters.; Correlations between MST markers, traditional FIB measurements, and physiochemical parameters indicate that the latter may be used to predict the likelihood of human- and canine-associated faecal inputs at this site.;
format	article
author	Serhat A. Yasar Toby J. T. Mills Zehra I. Uluturk Jason M. S. Ruszczyk Rebecca J. LeBard Brett A. Neilan
author_facet	Serhat A. Yasar Toby J. T. Mills Zehra I. Uluturk Jason M. S. Ruszczyk Rebecca J. LeBard Brett A. Neilan
author_sort	Serhat A. Yasar
title	Quantitative detection of human- and canine-associated Bacteroides genetic markers from an urban coastal lagoon
title_short	Quantitative detection of human- and canine-associated Bacteroides genetic markers from an urban coastal lagoon
title_full	Quantitative detection of human- and canine-associated Bacteroides genetic markers from an urban coastal lagoon
title_fullStr	Quantitative detection of human- and canine-associated Bacteroides genetic markers from an urban coastal lagoon
title_full_unstemmed	Quantitative detection of human- and canine-associated Bacteroides genetic markers from an urban coastal lagoon
title_sort	quantitative detection of human- and canine-associated bacteroides genetic markers from an urban coastal lagoon
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/d5300cd1639e434e98f59e90c2ec9662
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Quantitative detection of human- and canine-associated Bacteroides genetic markers from an urban coastal lagoon

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