Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells

Liver diseases are major causes of morbidity and mortality. Dental pulp pluripotent-like stem cells (DPPSCs) are of a considerable promise in tissue engineering and regenerative medicine as a new source of tissue-specific cells; therefore, this study is aimed at demonstrating their ability to genera...

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Autores principales:	Carlos Gil-Recio, Sheyla Montori, Saddam Al Demour, Mera A. Ababneh, Eduard Ferrés-Padró, Carles Marti, Elvira Ferrés-Amat, Miguel Barajas, Ashraf Al Madhoun, Maher Atari
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Internal medicine RC31-1245
Acceso en línea:	https://doaj.org/article/d530ec69fcd04f0fb7f344480fc2b042
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spelling	oai:doaj.org-article:d530ec69fcd04f0fb7f344480fc2b0422021-11-22T01:10:07ZChemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells1687-967810.1155/2021/5212852https://doaj.org/article/d530ec69fcd04f0fb7f344480fc2b0422021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/5212852https://doaj.org/toc/1687-9678Liver diseases are major causes of morbidity and mortality. Dental pulp pluripotent-like stem cells (DPPSCs) are of a considerable promise in tissue engineering and regenerative medicine as a new source of tissue-specific cells; therefore, this study is aimed at demonstrating their ability to generate functional hepatocyte-like cells in vitro. Cells were differentiated on a collagen scaffold in serum-free media supplemented with growth factors and cytokines to recapitulate liver development. At day 5, the differentiated DPPSC cells expressed the endodermal markers FOXA1 and FOXA2. Then, the cells were derived into the hepatic lineage generating hepatocyte-like cells. In addition to the associated morphological changes, the cells expressed the hepatic genes HNF6 and AFP. The terminally differentiated hepatocyte-like cells expressed the liver functional proteins albumin and CYP3A4. In this study, we report an efficient serum-free protocol to differentiate DPPSCs into functional hepatocyte-like cells. Our approach promotes the use of DPPSCs as a new source of adult stem cells for prospective use in liver regenerative medicine.Carlos Gil-RecioSheyla MontoriSaddam Al DemourMera A. AbabnehEduard Ferrés-PadróCarles MartiElvira Ferrés-AmatMiguel BarajasAshraf Al MadhounMaher AtariHindawi LimitedarticleInternal medicineRC31-1245ENStem Cells International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Internal medicine RC31-1245
spellingShingle	Internal medicine RC31-1245 Carlos Gil-Recio Sheyla Montori Saddam Al Demour Mera A. Ababneh Eduard Ferrés-Padró Carles Marti Elvira Ferrés-Amat Miguel Barajas Ashraf Al Madhoun Maher Atari Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells
description	Liver diseases are major causes of morbidity and mortality. Dental pulp pluripotent-like stem cells (DPPSCs) are of a considerable promise in tissue engineering and regenerative medicine as a new source of tissue-specific cells; therefore, this study is aimed at demonstrating their ability to generate functional hepatocyte-like cells in vitro. Cells were differentiated on a collagen scaffold in serum-free media supplemented with growth factors and cytokines to recapitulate liver development. At day 5, the differentiated DPPSC cells expressed the endodermal markers FOXA1 and FOXA2. Then, the cells were derived into the hepatic lineage generating hepatocyte-like cells. In addition to the associated morphological changes, the cells expressed the hepatic genes HNF6 and AFP. The terminally differentiated hepatocyte-like cells expressed the liver functional proteins albumin and CYP3A4. In this study, we report an efficient serum-free protocol to differentiate DPPSCs into functional hepatocyte-like cells. Our approach promotes the use of DPPSCs as a new source of adult stem cells for prospective use in liver regenerative medicine.
format	article
author	Carlos Gil-Recio Sheyla Montori Saddam Al Demour Mera A. Ababneh Eduard Ferrés-Padró Carles Marti Elvira Ferrés-Amat Miguel Barajas Ashraf Al Madhoun Maher Atari
author_facet	Carlos Gil-Recio Sheyla Montori Saddam Al Demour Mera A. Ababneh Eduard Ferrés-Padró Carles Marti Elvira Ferrés-Amat Miguel Barajas Ashraf Al Madhoun Maher Atari
author_sort	Carlos Gil-Recio
title	Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells
title_short	Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells
title_full	Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells
title_fullStr	Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells
title_full_unstemmed	Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells
title_sort	chemically defined conditions mediate an efficient induction of dental pulp pluripotent-like stem cells into hepatocyte-like cells
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/d530ec69fcd04f0fb7f344480fc2b042
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Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells

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