Effect of temperature on the rate of ageing: an experimental study of the blowfly Calliphora stygia.

All organisms age, the rate of which can be measured by demographic analysis of mortality rates. The rate of ageing is thermally sensitive in ectothermic invertebrates and we examined the effects of temperature on both demographic rates of ageing and on cellular senescence in the blowfly, Calliphora...

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Autores principales:	Megan A Kelly, Adam P Zieba, William A Buttemer, A J Hulbert
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d5337635ccaf4c959e2580b00063e495
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spelling	oai:doaj.org-article:d5337635ccaf4c959e2580b00063e4952021-11-18T08:57:10ZEffect of temperature on the rate of ageing: an experimental study of the blowfly Calliphora stygia.1932-620310.1371/journal.pone.0073781https://doaj.org/article/d5337635ccaf4c959e2580b00063e4952013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24019937/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203All organisms age, the rate of which can be measured by demographic analysis of mortality rates. The rate of ageing is thermally sensitive in ectothermic invertebrates and we examined the effects of temperature on both demographic rates of ageing and on cellular senescence in the blowfly, Calliphora stygia. The short lifespan of these flies is advantageous for demographic measurements while their large body size permits individual-based biochemical characterisation. Blowflies maintained at temperatures from 12°C to 34°C had a five to six-fold decrease in maximum and average longevity, respectively. Mortality rates were best described by a two-phase Gompertz relation, which revealed the first-phase of ageing to be much more temperature sensitive than the second stage. Flies held at low temperatures had both a slower first-phase rate of ageing and a delayed onset of second-phase ageing, which significantly extended their longevity compared with those at high temperatures. Blowflies that were transferred from 29°C to 15°C had higher first-phase mortality rates than those of flies held at constant 15°C, but their onset of second-phase ageing was deferred beyond that of flies held constantly at this temperature. The accumulation of fluorescent AGE pigment, a measure of cellular oxidative damage, increased steadily over time in all blowflies, irrespective of the temporal pattern of mortality. Pigment accumulated steadily during periods of 'negligible senescence', as measured by minimal rate of mortality, and the rate of accumulation was significantly affected by temperature. Thus accumulation of AGE pigment is more representative of chronological age than a reflection of biological age or a cause of mortality.Megan A KellyAdam P ZiebaWilliam A ButtemerA J HulbertPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e73781 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Megan A Kelly Adam P Zieba William A Buttemer A J Hulbert Effect of temperature on the rate of ageing: an experimental study of the blowfly Calliphora stygia.
description	All organisms age, the rate of which can be measured by demographic analysis of mortality rates. The rate of ageing is thermally sensitive in ectothermic invertebrates and we examined the effects of temperature on both demographic rates of ageing and on cellular senescence in the blowfly, Calliphora stygia. The short lifespan of these flies is advantageous for demographic measurements while their large body size permits individual-based biochemical characterisation. Blowflies maintained at temperatures from 12°C to 34°C had a five to six-fold decrease in maximum and average longevity, respectively. Mortality rates were best described by a two-phase Gompertz relation, which revealed the first-phase of ageing to be much more temperature sensitive than the second stage. Flies held at low temperatures had both a slower first-phase rate of ageing and a delayed onset of second-phase ageing, which significantly extended their longevity compared with those at high temperatures. Blowflies that were transferred from 29°C to 15°C had higher first-phase mortality rates than those of flies held at constant 15°C, but their onset of second-phase ageing was deferred beyond that of flies held constantly at this temperature. The accumulation of fluorescent AGE pigment, a measure of cellular oxidative damage, increased steadily over time in all blowflies, irrespective of the temporal pattern of mortality. Pigment accumulated steadily during periods of 'negligible senescence', as measured by minimal rate of mortality, and the rate of accumulation was significantly affected by temperature. Thus accumulation of AGE pigment is more representative of chronological age than a reflection of biological age or a cause of mortality.
format	article
author	Megan A Kelly Adam P Zieba William A Buttemer A J Hulbert
author_facet	Megan A Kelly Adam P Zieba William A Buttemer A J Hulbert
author_sort	Megan A Kelly
title	Effect of temperature on the rate of ageing: an experimental study of the blowfly Calliphora stygia.
title_short	Effect of temperature on the rate of ageing: an experimental study of the blowfly Calliphora stygia.
title_full	Effect of temperature on the rate of ageing: an experimental study of the blowfly Calliphora stygia.
title_fullStr	Effect of temperature on the rate of ageing: an experimental study of the blowfly Calliphora stygia.
title_full_unstemmed	Effect of temperature on the rate of ageing: an experimental study of the blowfly Calliphora stygia.
title_sort	effect of temperature on the rate of ageing: an experimental study of the blowfly calliphora stygia.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d5337635ccaf4c959e2580b00063e495
work_keys_str_mv	AT meganakelly effectoftemperatureontherateofageinganexperimentalstudyoftheblowflycalliphorastygia AT adampzieba effectoftemperatureontherateofageinganexperimentalstudyoftheblowflycalliphorastygia AT williamabuttemer effectoftemperatureontherateofageinganexperimentalstudyoftheblowflycalliphorastygia AT ajhulbert effectoftemperatureontherateofageinganexperimentalstudyoftheblowflycalliphorastygia
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Effect of temperature on the rate of ageing: an experimental study of the blowfly Calliphora stygia.

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