Identification of SFBB-containing canonical and noncanonical SCF complexes in pollen of apple (Malus × domestica).

Gametophytic self-incompatibility (GSI) of Rosaceae, Solanaceae and Plantaginaceae is controlled by a single polymorphic S locus. The S locus contains at least two genes, S-RNase and F-box protein encoding gene SLF/SFB/SFBB that control pistil and pollen specificity, respectively. Generally, the F-b...

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Autores principales:	Mai F Minamikawa, Ruriko Koyano, Shinji Kikuchi, Takato Koba, Hidenori Sassa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d535d663c72644edbefab0f347dea694
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spelling	oai:doaj.org-article:d535d663c72644edbefab0f347dea6942021-11-18T08:18:30ZIdentification of SFBB-containing canonical and noncanonical SCF complexes in pollen of apple (Malus × domestica).1932-620310.1371/journal.pone.0097642https://doaj.org/article/d535d663c72644edbefab0f347dea6942014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24847858/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Gametophytic self-incompatibility (GSI) of Rosaceae, Solanaceae and Plantaginaceae is controlled by a single polymorphic S locus. The S locus contains at least two genes, S-RNase and F-box protein encoding gene SLF/SFB/SFBB that control pistil and pollen specificity, respectively. Generally, the F-box protein forms an E3 ligase complex, SCF complex with Skp1, Cullin1 (CUL1) and Rbx1, however, in Petunia inflata, SBP1 (S-RNase binding protein1) was reported to play the role of Skp1 and Rbx1, and form an SCFSLF-like complex for ubiquitination of non-self S-RNases. On the other hand, in Petunia hybrida and Petunia inflata of Solanaceae, Prunus avium and Pyrus bretschneideri of Rosaceae, SSK1 (SLF-interacting Skp1-like protein1) is considered to form the SCFSLF/SFB complex. Here, we isolated pollen-expressed apple homologs of SSK1 and CUL1, and named MdSSK1, MdCUL1A and MdCUL1B. MdSSK1 was preferentially expressed in pollen, but weakly in other organs analyzed, while, MdCUL1A and MdCUL1B were almost equally expressed in all the organs analyzed. MdSSK1 transcript abundance was significantly (>100 times) higher than that of MdSBP1. In vitro binding assays showed that MdSSK1 and MdSBP1 interacted with MdSFBB1-S9 and MdCUL1, and MdSFBB1-S9 interacted more strongly with MdSSK1 than with MdSBP1. The results suggest that both MdSSK1-containing SCFSFBB1 and MdSBP1-containing SCFSFBB1-like complexes function in pollen of apple, and the former plays a major role.Mai F MinamikawaRuriko KoyanoShinji KikuchiTakato KobaHidenori SassaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e97642 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mai F Minamikawa Ruriko Koyano Shinji Kikuchi Takato Koba Hidenori Sassa Identification of SFBB-containing canonical and noncanonical SCF complexes in pollen of apple (Malus × domestica).
description	Gametophytic self-incompatibility (GSI) of Rosaceae, Solanaceae and Plantaginaceae is controlled by a single polymorphic S locus. The S locus contains at least two genes, S-RNase and F-box protein encoding gene SLF/SFB/SFBB that control pistil and pollen specificity, respectively. Generally, the F-box protein forms an E3 ligase complex, SCF complex with Skp1, Cullin1 (CUL1) and Rbx1, however, in Petunia inflata, SBP1 (S-RNase binding protein1) was reported to play the role of Skp1 and Rbx1, and form an SCFSLF-like complex for ubiquitination of non-self S-RNases. On the other hand, in Petunia hybrida and Petunia inflata of Solanaceae, Prunus avium and Pyrus bretschneideri of Rosaceae, SSK1 (SLF-interacting Skp1-like protein1) is considered to form the SCFSLF/SFB complex. Here, we isolated pollen-expressed apple homologs of SSK1 and CUL1, and named MdSSK1, MdCUL1A and MdCUL1B. MdSSK1 was preferentially expressed in pollen, but weakly in other organs analyzed, while, MdCUL1A and MdCUL1B were almost equally expressed in all the organs analyzed. MdSSK1 transcript abundance was significantly (>100 times) higher than that of MdSBP1. In vitro binding assays showed that MdSSK1 and MdSBP1 interacted with MdSFBB1-S9 and MdCUL1, and MdSFBB1-S9 interacted more strongly with MdSSK1 than with MdSBP1. The results suggest that both MdSSK1-containing SCFSFBB1 and MdSBP1-containing SCFSFBB1-like complexes function in pollen of apple, and the former plays a major role.
format	article
author	Mai F Minamikawa Ruriko Koyano Shinji Kikuchi Takato Koba Hidenori Sassa
author_facet	Mai F Minamikawa Ruriko Koyano Shinji Kikuchi Takato Koba Hidenori Sassa
author_sort	Mai F Minamikawa
title	Identification of SFBB-containing canonical and noncanonical SCF complexes in pollen of apple (Malus × domestica).
title_short	Identification of SFBB-containing canonical and noncanonical SCF complexes in pollen of apple (Malus × domestica).
title_full	Identification of SFBB-containing canonical and noncanonical SCF complexes in pollen of apple (Malus × domestica).
title_fullStr	Identification of SFBB-containing canonical and noncanonical SCF complexes in pollen of apple (Malus × domestica).
title_full_unstemmed	Identification of SFBB-containing canonical and noncanonical SCF complexes in pollen of apple (Malus × domestica).
title_sort	identification of sfbb-containing canonical and noncanonical scf complexes in pollen of apple (malus × domestica).
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d535d663c72644edbefab0f347dea694
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Identification of SFBB-containing canonical and noncanonical SCF complexes in pollen of apple (Malus × domestica).

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