Single-cell protein-mRNA correlation analysis enabled by multiplexed dual-analyte co-detection

Abstract We have investigated the correlation between proteins and mRNAs in single cells employing an integrated workflow for dual-analyte co-detection. This is achieved by combining the oligo extension reaction (OER), which converts protein levels to DNA levels, with reverse transcription for mRNA...

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Autores principales:	Haibiao Gong, Xiaohui Wang, Benjamin Liu, Stephane Boutet, Ilona Holcomb, Gajalakshmi Dakshinamoorthy, Aik Ooi, Chad Sanada, Gang Sun, Ramesh Ramakrishnan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d53b4a806b534842a54ad55e4e3c21e5
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spelling	oai:doaj.org-article:d53b4a806b534842a54ad55e4e3c21e52021-12-02T15:06:19ZSingle-cell protein-mRNA correlation analysis enabled by multiplexed dual-analyte co-detection10.1038/s41598-017-03057-52045-2322https://doaj.org/article/d53b4a806b534842a54ad55e4e3c21e52017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03057-5https://doaj.org/toc/2045-2322Abstract We have investigated the correlation between proteins and mRNAs in single cells employing an integrated workflow for dual-analyte co-detection. This is achieved by combining the oligo extension reaction (OER), which converts protein levels to DNA levels, with reverse transcription for mRNA detection. Unsupervised gene expression profiling analysis, including principal component analysis and hierarchical clustering, revealed different aspects of the protein-mRNA relationship. Violin plot analysis showed that some genes exhibited similar distribution patterns for proteins and mRNAs. We also demonstrate that cells can be separated into subpopulations based on their protein-mRNA expression profiles, and that different subpopulations have distinct correlation coefficient values. Our results demonstrated that integrated investigations of mRNA and protein levels in single cells allows comprehensive analysis not attainable at bulk levels.Haibiao GongXiaohui WangBenjamin LiuStephane BoutetIlona HolcombGajalakshmi DakshinamoorthyAik OoiChad SanadaGang SunRamesh RamakrishnanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haibiao Gong Xiaohui Wang Benjamin Liu Stephane Boutet Ilona Holcomb Gajalakshmi Dakshinamoorthy Aik Ooi Chad Sanada Gang Sun Ramesh Ramakrishnan Single-cell protein-mRNA correlation analysis enabled by multiplexed dual-analyte co-detection
description	Abstract We have investigated the correlation between proteins and mRNAs in single cells employing an integrated workflow for dual-analyte co-detection. This is achieved by combining the oligo extension reaction (OER), which converts protein levels to DNA levels, with reverse transcription for mRNA detection. Unsupervised gene expression profiling analysis, including principal component analysis and hierarchical clustering, revealed different aspects of the protein-mRNA relationship. Violin plot analysis showed that some genes exhibited similar distribution patterns for proteins and mRNAs. We also demonstrate that cells can be separated into subpopulations based on their protein-mRNA expression profiles, and that different subpopulations have distinct correlation coefficient values. Our results demonstrated that integrated investigations of mRNA and protein levels in single cells allows comprehensive analysis not attainable at bulk levels.
format	article
author	Haibiao Gong Xiaohui Wang Benjamin Liu Stephane Boutet Ilona Holcomb Gajalakshmi Dakshinamoorthy Aik Ooi Chad Sanada Gang Sun Ramesh Ramakrishnan
author_facet	Haibiao Gong Xiaohui Wang Benjamin Liu Stephane Boutet Ilona Holcomb Gajalakshmi Dakshinamoorthy Aik Ooi Chad Sanada Gang Sun Ramesh Ramakrishnan
author_sort	Haibiao Gong
title	Single-cell protein-mRNA correlation analysis enabled by multiplexed dual-analyte co-detection
title_short	Single-cell protein-mRNA correlation analysis enabled by multiplexed dual-analyte co-detection
title_full	Single-cell protein-mRNA correlation analysis enabled by multiplexed dual-analyte co-detection
title_fullStr	Single-cell protein-mRNA correlation analysis enabled by multiplexed dual-analyte co-detection
title_full_unstemmed	Single-cell protein-mRNA correlation analysis enabled by multiplexed dual-analyte co-detection
title_sort	single-cell protein-mrna correlation analysis enabled by multiplexed dual-analyte co-detection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d53b4a806b534842a54ad55e4e3c21e5
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Single-cell protein-mRNA correlation analysis enabled by multiplexed dual-analyte co-detection

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