Monocytes from depressed patients display an altered pattern of response to endotoxin challenge.

It is now well established that major depression is accompanied and characterized by altered responses of the immune-inflammatory system. In this study we investigated the pro-inflammatory activation of monocytes isolated from depressed patients as a parameter not influenced by such confounds as the...

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Autores principales:	Lucia Lisi, Giovanni Camardese, Mariangela Treglia, Giuseppe Tringali, Cinzia Carrozza, Luigi Janiri, Cinzia Dello Russo, Pierluigi Navarra
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d53d7bca35a247e3abce8f9a915ab9d1
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spelling	oai:doaj.org-article:d53d7bca35a247e3abce8f9a915ab9d12021-11-18T08:02:48ZMonocytes from depressed patients display an altered pattern of response to endotoxin challenge.1932-620310.1371/journal.pone.0052585https://doaj.org/article/d53d7bca35a247e3abce8f9a915ab9d12013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23300980/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203It is now well established that major depression is accompanied and characterized by altered responses of the immune-inflammatory system. In this study we investigated the pro-inflammatory activation of monocytes isolated from depressed patients as a parameter not influenced by such confounds as the time of day, the nutritional and exercise status or the age and gender of patients. Monocytes from depressed patients and from healthy controls were isolated in vitro; after 24-h incubation under basal conditions, cells were exposed for 24-h to 100 ng/ml of endotoxin (bacterial lipopolysaccharide, LPS). We found that monocytes from drug-free depressed patients and controls release the same amounts of prostaglandin E2 (PGE2) under basal conditions, whereas monocytes from patients are dramatically less reactive to LPS (8.62-fold increase vs previous 24 hrs) compared to healthy controls (123.3-fold increase vs previous 24 hrs). Such blunted prostanoid production was paralleled by a reduction in COX-2 gene expression, whereas other pro-inflammatory mediators, namely interleukin-1β (IL-1 β) and -6 (IL-6) showed a trend to increased gene expression. The above changes were not associated to increased levels of circulating glucocorticoids. After 8 months of antidepressive drug treatment, the increase in PGE2 production after the endotoxin challenge was partially restored, whereas the increase in IL-1 β and -6 levels observed at baseline was completely abolished. In conclusion, our findings show that the reactivity of monocytes from depressed patients might be considered as a marker of the immune-inflammatory disorders associated to depression, although the lack of paired healthy controls at follow-up does not allow to conclude that monocyte reactivity to endotoxin is also a marker of treatment outcome.Lucia LisiGiovanni CamardeseMariangela TregliaGiuseppe TringaliCinzia CarrozzaLuigi JaniriCinzia Dello RussoPierluigi NavarraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e52585 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucia Lisi Giovanni Camardese Mariangela Treglia Giuseppe Tringali Cinzia Carrozza Luigi Janiri Cinzia Dello Russo Pierluigi Navarra Monocytes from depressed patients display an altered pattern of response to endotoxin challenge.
description	It is now well established that major depression is accompanied and characterized by altered responses of the immune-inflammatory system. In this study we investigated the pro-inflammatory activation of monocytes isolated from depressed patients as a parameter not influenced by such confounds as the time of day, the nutritional and exercise status or the age and gender of patients. Monocytes from depressed patients and from healthy controls were isolated in vitro; after 24-h incubation under basal conditions, cells were exposed for 24-h to 100 ng/ml of endotoxin (bacterial lipopolysaccharide, LPS). We found that monocytes from drug-free depressed patients and controls release the same amounts of prostaglandin E2 (PGE2) under basal conditions, whereas monocytes from patients are dramatically less reactive to LPS (8.62-fold increase vs previous 24 hrs) compared to healthy controls (123.3-fold increase vs previous 24 hrs). Such blunted prostanoid production was paralleled by a reduction in COX-2 gene expression, whereas other pro-inflammatory mediators, namely interleukin-1β (IL-1 β) and -6 (IL-6) showed a trend to increased gene expression. The above changes were not associated to increased levels of circulating glucocorticoids. After 8 months of antidepressive drug treatment, the increase in PGE2 production after the endotoxin challenge was partially restored, whereas the increase in IL-1 β and -6 levels observed at baseline was completely abolished. In conclusion, our findings show that the reactivity of monocytes from depressed patients might be considered as a marker of the immune-inflammatory disorders associated to depression, although the lack of paired healthy controls at follow-up does not allow to conclude that monocyte reactivity to endotoxin is also a marker of treatment outcome.
format	article
author	Lucia Lisi Giovanni Camardese Mariangela Treglia Giuseppe Tringali Cinzia Carrozza Luigi Janiri Cinzia Dello Russo Pierluigi Navarra
author_facet	Lucia Lisi Giovanni Camardese Mariangela Treglia Giuseppe Tringali Cinzia Carrozza Luigi Janiri Cinzia Dello Russo Pierluigi Navarra
author_sort	Lucia Lisi
title	Monocytes from depressed patients display an altered pattern of response to endotoxin challenge.
title_short	Monocytes from depressed patients display an altered pattern of response to endotoxin challenge.
title_full	Monocytes from depressed patients display an altered pattern of response to endotoxin challenge.
title_fullStr	Monocytes from depressed patients display an altered pattern of response to endotoxin challenge.
title_full_unstemmed	Monocytes from depressed patients display an altered pattern of response to endotoxin challenge.
title_sort	monocytes from depressed patients display an altered pattern of response to endotoxin challenge.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d53d7bca35a247e3abce8f9a915ab9d1
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Monocytes from depressed patients display an altered pattern of response to endotoxin challenge.

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