Isolated Bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing PCR

Abstract Plant growth-promoting bacteria (PGPB) may trigger tolerance against biotic/abiotic stresses and growth enhancement in plants. In this study, an endophytic bacterial strain from rapeseed was isolated to assess its role in enhancing plant growth and tolerance to abiotic stresses, as well as...

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Autores principales:	Zahoor Ahmad, Jia Wu, Lulu Chen, Wubei Dong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d5427b2a3b134892bdc54592509d3175
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spelling	oai:doaj.org-article:d5427b2a3b134892bdc54592509d31752021-12-02T15:06:15ZIsolated Bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing PCR10.1038/s41598-017-01940-92045-2322https://doaj.org/article/d5427b2a3b134892bdc54592509d31752017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01940-9https://doaj.org/toc/2045-2322Abstract Plant growth-promoting bacteria (PGPB) may trigger tolerance against biotic/abiotic stresses and growth enhancement in plants. In this study, an endophytic bacterial strain from rapeseed was isolated to assess its role in enhancing plant growth and tolerance to abiotic stresses, as well as banded leaf and sheath blight disease in maize. Based on 16S rDNA and BIOLOG test analysis, the 330-2 strain was identified as Bacillus subtilis. The strain produced indole-3-acetic acid, siderophores, lytic enzymes and solubilized different sources of organic/inorganic phosphates and zinc. Furthermore, the strain strongly suppressed the in vitro growth of Rhizoctonia solani AG1-IA, Botrytis cinerea, Fusarium oxysporum, Alternaria alternata, Cochliobolus heterostrophus, and Nigrospora oryzae. The strain also significantly increased the seedling growth (ranging 14–37%) of rice and maize. Removing PCR analysis indicated that 114 genes were differentially expressed, among which 10%, 32% and 10% were involved in antibiotic production (e.g., srfAA, bae, fen, mln, and dfnI), metabolism (e.g., gltA, pabA, and ggt) and transportation of nutrients (e.g., fhu, glpT, and gltT), respectively. In summary, these results clearly indicate the effectiveness and mechanisms of B. subtilis strain 330-2 in enhancing plant growth, as well as tolerance to biotic/abiotic stresses, which suggests that the strain has great potential for commercialization as a vital biological control agent.Zahoor AhmadJia WuLulu ChenWubei DongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zahoor Ahmad Jia Wu Lulu Chen Wubei Dong Isolated Bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing PCR
description	Abstract Plant growth-promoting bacteria (PGPB) may trigger tolerance against biotic/abiotic stresses and growth enhancement in plants. In this study, an endophytic bacterial strain from rapeseed was isolated to assess its role in enhancing plant growth and tolerance to abiotic stresses, as well as banded leaf and sheath blight disease in maize. Based on 16S rDNA and BIOLOG test analysis, the 330-2 strain was identified as Bacillus subtilis. The strain produced indole-3-acetic acid, siderophores, lytic enzymes and solubilized different sources of organic/inorganic phosphates and zinc. Furthermore, the strain strongly suppressed the in vitro growth of Rhizoctonia solani AG1-IA, Botrytis cinerea, Fusarium oxysporum, Alternaria alternata, Cochliobolus heterostrophus, and Nigrospora oryzae. The strain also significantly increased the seedling growth (ranging 14–37%) of rice and maize. Removing PCR analysis indicated that 114 genes were differentially expressed, among which 10%, 32% and 10% were involved in antibiotic production (e.g., srfAA, bae, fen, mln, and dfnI), metabolism (e.g., gltA, pabA, and ggt) and transportation of nutrients (e.g., fhu, glpT, and gltT), respectively. In summary, these results clearly indicate the effectiveness and mechanisms of B. subtilis strain 330-2 in enhancing plant growth, as well as tolerance to biotic/abiotic stresses, which suggests that the strain has great potential for commercialization as a vital biological control agent.
format	article
author	Zahoor Ahmad Jia Wu Lulu Chen Wubei Dong
author_facet	Zahoor Ahmad Jia Wu Lulu Chen Wubei Dong
author_sort	Zahoor Ahmad
title	Isolated Bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing PCR
title_short	Isolated Bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing PCR
title_full	Isolated Bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing PCR
title_fullStr	Isolated Bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing PCR
title_full_unstemmed	Isolated Bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing PCR
title_sort	isolated bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing pcr
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d5427b2a3b134892bdc54592509d3175
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Isolated Bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing PCR

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