Metabolite Profiling for Six 'B' Vitamins Using LC-MS in Tomato Genotypes at Different Stages of Fruit Maturity

Vitamins are essential nutrients in food crucial for maintaining good health. Tomato, being a widely consumed vegetable, provides a good quantity of vitamins. Metabolite profiling of vitamins at different stages of fruit maturity in a crop helps identify the right stage for better quality. Based on...

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Autores principales:	P Kavitha, K S Shivashankara, T K Roy, K C Pavithra, V K Rao, A T Sadashiva, K V Ravishankar, G J Sathish
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2015
Materias:	tomato b vitamins lc-ms/ms-mrm fruit ripening green stage breaker stage Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/d544c096cce94e6895abc1c0462a6869
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spelling	oai:doaj.org-article:d544c096cce94e6895abc1c0462a68692021-12-02T04:02:03ZMetabolite Profiling for Six 'B' Vitamins Using LC-MS in Tomato Genotypes at Different Stages of Fruit Maturity0973-354X2582-4899https://doaj.org/article/d544c096cce94e6895abc1c0462a68692015-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/150https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899Vitamins are essential nutrients in food crucial for maintaining good health. Tomato, being a widely consumed vegetable, provides a good quantity of vitamins. Metabolite profiling of vitamins at different stages of fruit maturity in a crop helps identify the right stage for better quality. Based on preliminary screening for quality parameters, tomato lines rich in TSS, antioxidants, lycopene and beta-carotene were selected for the present study. Eight genotypes and a wild species were profiled for 'B' vitamins at three different stages of fruit maturity, viz., green, breaker and ripe stage. A simple and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for simultaneous determination of six 'B' vitamins was developed and validated by us. Among the genotypes studied, IIHR-249-1 recorded higher niacin, pantothenic acid and biotin content. Pyridoxine content was higher in the hybrid, Arka Rakshak. The wild species, LA-1777(Solanum habrochaites) was found to be rich in pantothenic acid, riboflavin and thiamine. Content of most of the vitamins increased with ripening of the fruit. IIHR-249-1 and LA-1777 were found to be rich in 'B' vitamins, earlier reported to be also rich in antioxidants and lycopene. These genotypes can be used for improving the nutritive value of tomato under crop improvement programmes, through conventional breeding or biotechnological approaches.P KavithaK S ShivashankaraT K RoyK C PavithraV K RaoA T SadashivaK V RavishankarG J SathishSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticletomatob vitaminslc-ms/ms-mrmfruit ripeninggreen stagebreaker stagePlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 10, Iss 1, Pp 30-37 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	tomato b vitamins lc-ms/ms-mrm fruit ripening green stage breaker stage Plant culture SB1-1110
spellingShingle	tomato b vitamins lc-ms/ms-mrm fruit ripening green stage breaker stage Plant culture SB1-1110 P Kavitha K S Shivashankara T K Roy K C Pavithra V K Rao A T Sadashiva K V Ravishankar G J Sathish Metabolite Profiling for Six 'B' Vitamins Using LC-MS in Tomato Genotypes at Different Stages of Fruit Maturity
description	Vitamins are essential nutrients in food crucial for maintaining good health. Tomato, being a widely consumed vegetable, provides a good quantity of vitamins. Metabolite profiling of vitamins at different stages of fruit maturity in a crop helps identify the right stage for better quality. Based on preliminary screening for quality parameters, tomato lines rich in TSS, antioxidants, lycopene and beta-carotene were selected for the present study. Eight genotypes and a wild species were profiled for 'B' vitamins at three different stages of fruit maturity, viz., green, breaker and ripe stage. A simple and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for simultaneous determination of six 'B' vitamins was developed and validated by us. Among the genotypes studied, IIHR-249-1 recorded higher niacin, pantothenic acid and biotin content. Pyridoxine content was higher in the hybrid, Arka Rakshak. The wild species, LA-1777(Solanum habrochaites) was found to be rich in pantothenic acid, riboflavin and thiamine. Content of most of the vitamins increased with ripening of the fruit. IIHR-249-1 and LA-1777 were found to be rich in 'B' vitamins, earlier reported to be also rich in antioxidants and lycopene. These genotypes can be used for improving the nutritive value of tomato under crop improvement programmes, through conventional breeding or biotechnological approaches.
format	article
author	P Kavitha K S Shivashankara T K Roy K C Pavithra V K Rao A T Sadashiva K V Ravishankar G J Sathish
author_facet	P Kavitha K S Shivashankara T K Roy K C Pavithra V K Rao A T Sadashiva K V Ravishankar G J Sathish
author_sort	P Kavitha
title	Metabolite Profiling for Six 'B' Vitamins Using LC-MS in Tomato Genotypes at Different Stages of Fruit Maturity
title_short	Metabolite Profiling for Six 'B' Vitamins Using LC-MS in Tomato Genotypes at Different Stages of Fruit Maturity
title_full	Metabolite Profiling for Six 'B' Vitamins Using LC-MS in Tomato Genotypes at Different Stages of Fruit Maturity
title_fullStr	Metabolite Profiling for Six 'B' Vitamins Using LC-MS in Tomato Genotypes at Different Stages of Fruit Maturity
title_full_unstemmed	Metabolite Profiling for Six 'B' Vitamins Using LC-MS in Tomato Genotypes at Different Stages of Fruit Maturity
title_sort	metabolite profiling for six 'b' vitamins using lc-ms in tomato genotypes at different stages of fruit maturity
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2015
url	https://doaj.org/article/d544c096cce94e6895abc1c0462a6869
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Metabolite Profiling for Six 'B' Vitamins Using LC-MS in Tomato Genotypes at Different Stages of Fruit Maturity

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