Blooming gelatin: an individual additive for enhancing nanoapatite precipitation, physical properties, and osteoblastic responses of nanostructured macroporous calcium phosphate bone cements

Ziba Orshesh, Saeed Hesaraki, Ali Khanlarkhani Biomaterials Group, Nanotechnology and Advanced Materials Department, Materials and Energy Research Center, Alborz, Iran Abstract: In recent years, there has been a great interest in using natural polymers in the composition of calcium phosphate bone...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Orshesh Z, Hesaraki S, Khanlarkhani A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	calcium phosphate cement nanoapatite cell studies macroporosity bone substitute blooming gelatin Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d550c61ed8634a8b840f6759ca1bba6f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d550c61ed8634a8b840f6759ca1bba6f
record_format	dspace
spelling	oai:doaj.org-article:d550c61ed8634a8b840f6759ca1bba6f2021-12-02T06:47:14ZBlooming gelatin: an individual additive for enhancing nanoapatite precipitation, physical properties, and osteoblastic responses of nanostructured macroporous calcium phosphate bone cements1178-2013https://doaj.org/article/d550c61ed8634a8b840f6759ca1bba6f2017-01-01T00:00:00Zhttps://www.dovepress.com/blooming-gelatin-an-individual-additive-for-enhancing-nanoapatite-prec-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ziba Orshesh, Saeed Hesaraki, Ali Khanlarkhani Biomaterials Group, Nanotechnology and Advanced Materials Department, Materials and Energy Research Center, Alborz, Iran Abstract: In recent years, there has been a great interest in using natural polymers in the composition of calcium phosphate bone cements to enhance their physical, mechanical, and biological performance. Gelatin is a partially hydrolyzed form of collagen, a natural component of bone matrix. In this study, the effect of blooming gelatin on the nanohydroxyapatite precipitation, physical and mechanical properties, and cellular responses of a calcium phosphate bone cement (CPC) was investigated. Various concentrations of blooming gelatin (2, 5, and 8 wt.%) were used as the cement liquid and an equimolar mixture of tetracalcium phosphate and dicalcium phosphate was used as solid phase. The CPC without any gelatin additive was also evaluated as a control group. The results showed that gelatin accelerated hydraulic reactions of the cement paste, in which the reactants were immediately converted into nanostructured apatite precipitates after hardening. Gelatin molecules induced 4%–10% macropores (10–300 µm) into the cement structure, decreased initial setting time by ~190%, and improved mechanical strength of the as-set cement. Variation in the above-mentioned properties was influenced by the gelatin concentration and progressed with increasing the gelatin content. The numbers of the G-292 osteoblastic cells on gelatin-containing CPCs were higher than the control group at entire culture times (1–14 days), meanwhile better alkaline phosphatase (ALP) activity was determined using blooming gelatin additive. The observation of cell morphologies on the cement surfaces revealed an appropriate cell attachment with extended cell membranes on the cements. Overall, adding gelatin to the composition of CPC improved the handling characteristics such as setting time and mechanical properties, enhanced nanoapatite precipitation, and augmented the early cell proliferation rate and ALP activity. Keywords: calcium phosphate cement, nanoapatite, cell studies, macroporosity, bone substitute, blooming gelatinOrshesh ZHesaraki SKhanlarkhani ADove Medical Pressarticlecalcium phosphate cementnanoapatitecell studiesmacroporositybone substituteblooming gelatinMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 745-758 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	calcium phosphate cement nanoapatite cell studies macroporosity bone substitute blooming gelatin Medicine (General) R5-920
spellingShingle	calcium phosphate cement nanoapatite cell studies macroporosity bone substitute blooming gelatin Medicine (General) R5-920 Orshesh Z Hesaraki S Khanlarkhani A Blooming gelatin: an individual additive for enhancing nanoapatite precipitation, physical properties, and osteoblastic responses of nanostructured macroporous calcium phosphate bone cements
description	Ziba Orshesh, Saeed Hesaraki, Ali Khanlarkhani Biomaterials Group, Nanotechnology and Advanced Materials Department, Materials and Energy Research Center, Alborz, Iran Abstract: In recent years, there has been a great interest in using natural polymers in the composition of calcium phosphate bone cements to enhance their physical, mechanical, and biological performance. Gelatin is a partially hydrolyzed form of collagen, a natural component of bone matrix. In this study, the effect of blooming gelatin on the nanohydroxyapatite precipitation, physical and mechanical properties, and cellular responses of a calcium phosphate bone cement (CPC) was investigated. Various concentrations of blooming gelatin (2, 5, and 8 wt.%) were used as the cement liquid and an equimolar mixture of tetracalcium phosphate and dicalcium phosphate was used as solid phase. The CPC without any gelatin additive was also evaluated as a control group. The results showed that gelatin accelerated hydraulic reactions of the cement paste, in which the reactants were immediately converted into nanostructured apatite precipitates after hardening. Gelatin molecules induced 4%–10% macropores (10–300 µm) into the cement structure, decreased initial setting time by ~190%, and improved mechanical strength of the as-set cement. Variation in the above-mentioned properties was influenced by the gelatin concentration and progressed with increasing the gelatin content. The numbers of the G-292 osteoblastic cells on gelatin-containing CPCs were higher than the control group at entire culture times (1–14 days), meanwhile better alkaline phosphatase (ALP) activity was determined using blooming gelatin additive. The observation of cell morphologies on the cement surfaces revealed an appropriate cell attachment with extended cell membranes on the cements. Overall, adding gelatin to the composition of CPC improved the handling characteristics such as setting time and mechanical properties, enhanced nanoapatite precipitation, and augmented the early cell proliferation rate and ALP activity. Keywords: calcium phosphate cement, nanoapatite, cell studies, macroporosity, bone substitute, blooming gelatin
format	article
author	Orshesh Z Hesaraki S Khanlarkhani A
author_facet	Orshesh Z Hesaraki S Khanlarkhani A
author_sort	Orshesh Z
title	Blooming gelatin: an individual additive for enhancing nanoapatite precipitation, physical properties, and osteoblastic responses of nanostructured macroporous calcium phosphate bone cements
title_short	Blooming gelatin: an individual additive for enhancing nanoapatite precipitation, physical properties, and osteoblastic responses of nanostructured macroporous calcium phosphate bone cements
title_full	Blooming gelatin: an individual additive for enhancing nanoapatite precipitation, physical properties, and osteoblastic responses of nanostructured macroporous calcium phosphate bone cements
title_fullStr	Blooming gelatin: an individual additive for enhancing nanoapatite precipitation, physical properties, and osteoblastic responses of nanostructured macroporous calcium phosphate bone cements
title_full_unstemmed	Blooming gelatin: an individual additive for enhancing nanoapatite precipitation, physical properties, and osteoblastic responses of nanostructured macroporous calcium phosphate bone cements
title_sort	blooming gelatin: an individual additive for enhancing nanoapatite precipitation, physical properties, and osteoblastic responses of nanostructured macroporous calcium phosphate bone cements
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/d550c61ed8634a8b840f6759ca1bba6f
work_keys_str_mv	AT orsheshz bloominggelatinanindividualadditiveforenhancingnanoapatiteprecipitationphysicalpropertiesandosteoblasticresponsesofnanostructuredmacroporouscalciumphosphatebonecements AT hesarakis bloominggelatinanindividualadditiveforenhancingnanoapatiteprecipitationphysicalpropertiesandosteoblasticresponsesofnanostructuredmacroporouscalciumphosphatebonecements AT khanlarkhania bloominggelatinanindividualadditiveforenhancingnanoapatiteprecipitationphysicalpropertiesandosteoblasticresponsesofnanostructuredmacroporouscalciumphosphatebonecements
_version_	1718399781488820224

Blooming gelatin: an individual additive for enhancing nanoapatite precipitation, physical properties, and osteoblastic responses of nanostructured macroporous calcium phosphate bone cements

Ejemplares similares