Serum Metabolomic Profiles for Breast Cancer Diagnosis, Grading and Staging by Gas Chromatography-Mass Spectrometry

Abstract Detection of metabolic signature for breast cancer (BC) has the potential to improve patient prognosis. This study identified potentially significant metabolites differentiating between breast cancer patients and healthy controls to help in diagnosis, grading, staging and determination of n...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Naila Irum Hadi, Qamar Jamal, Ayesha Iqbal, Fouzia Shaikh, Saleem Somroo, Syed Ghulam Musharraf
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
Materias:
R
Q
Acceso en línea:https://doaj.org/article/d55728b912824135a3a7a25483e17c90
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:d55728b912824135a3a7a25483e17c90
record_format dspace
spelling oai:doaj.org-article:d55728b912824135a3a7a25483e17c902021-12-02T12:31:46ZSerum Metabolomic Profiles for Breast Cancer Diagnosis, Grading and Staging by Gas Chromatography-Mass Spectrometry10.1038/s41598-017-01924-92045-2322https://doaj.org/article/d55728b912824135a3a7a25483e17c902017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01924-9https://doaj.org/toc/2045-2322Abstract Detection of metabolic signature for breast cancer (BC) has the potential to improve patient prognosis. This study identified potentially significant metabolites differentiating between breast cancer patients and healthy controls to help in diagnosis, grading, staging and determination of neoadjuvant status. Serum was collected from 152 pre-operative breast cancer (BC) patients and 155 healthy controls in this case-controlled study. Gas chromatography-mass spectrometry (GC-MS) was used to obtain metabolic profiles followed by chemometric analysis with the identification of significantly differentiated metabolites including 7 for diagnosis, 18 for grading, 23 for staging, 15 for stage III subcategory and 10 for neoadjuvant status (p-value < 0.05). Partial Least Square Discriminant Analysis (PLS-DA) model revealed a distinct separation between healthy controls and BC patients with a sensitivity of 96% and specificity of 100% on external validation. Models for grading, staging and neoadjuvant status were built with Decision Tree Algorithm with predictive accuracy of 71.5%, 71.3% and 79.8% respectively. Pathway analysis revealed increased glycolysis, lipogenesis, and production of volatile organic metabolites indicating the metabolic alterations in breast cancer.Naila Irum HadiQamar JamalAyesha IqbalFouzia ShaikhSaleem SomrooSyed Ghulam MusharrafNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Naila Irum Hadi
Qamar Jamal
Ayesha Iqbal
Fouzia Shaikh
Saleem Somroo
Syed Ghulam Musharraf
Serum Metabolomic Profiles for Breast Cancer Diagnosis, Grading and Staging by Gas Chromatography-Mass Spectrometry
description Abstract Detection of metabolic signature for breast cancer (BC) has the potential to improve patient prognosis. This study identified potentially significant metabolites differentiating between breast cancer patients and healthy controls to help in diagnosis, grading, staging and determination of neoadjuvant status. Serum was collected from 152 pre-operative breast cancer (BC) patients and 155 healthy controls in this case-controlled study. Gas chromatography-mass spectrometry (GC-MS) was used to obtain metabolic profiles followed by chemometric analysis with the identification of significantly differentiated metabolites including 7 for diagnosis, 18 for grading, 23 for staging, 15 for stage III subcategory and 10 for neoadjuvant status (p-value < 0.05). Partial Least Square Discriminant Analysis (PLS-DA) model revealed a distinct separation between healthy controls and BC patients with a sensitivity of 96% and specificity of 100% on external validation. Models for grading, staging and neoadjuvant status were built with Decision Tree Algorithm with predictive accuracy of 71.5%, 71.3% and 79.8% respectively. Pathway analysis revealed increased glycolysis, lipogenesis, and production of volatile organic metabolites indicating the metabolic alterations in breast cancer.
format article
author Naila Irum Hadi
Qamar Jamal
Ayesha Iqbal
Fouzia Shaikh
Saleem Somroo
Syed Ghulam Musharraf
author_facet Naila Irum Hadi
Qamar Jamal
Ayesha Iqbal
Fouzia Shaikh
Saleem Somroo
Syed Ghulam Musharraf
author_sort Naila Irum Hadi
title Serum Metabolomic Profiles for Breast Cancer Diagnosis, Grading and Staging by Gas Chromatography-Mass Spectrometry
title_short Serum Metabolomic Profiles for Breast Cancer Diagnosis, Grading and Staging by Gas Chromatography-Mass Spectrometry
title_full Serum Metabolomic Profiles for Breast Cancer Diagnosis, Grading and Staging by Gas Chromatography-Mass Spectrometry
title_fullStr Serum Metabolomic Profiles for Breast Cancer Diagnosis, Grading and Staging by Gas Chromatography-Mass Spectrometry
title_full_unstemmed Serum Metabolomic Profiles for Breast Cancer Diagnosis, Grading and Staging by Gas Chromatography-Mass Spectrometry
title_sort serum metabolomic profiles for breast cancer diagnosis, grading and staging by gas chromatography-mass spectrometry
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/d55728b912824135a3a7a25483e17c90
work_keys_str_mv AT nailairumhadi serummetabolomicprofilesforbreastcancerdiagnosisgradingandstagingbygaschromatographymassspectrometry
AT qamarjamal serummetabolomicprofilesforbreastcancerdiagnosisgradingandstagingbygaschromatographymassspectrometry
AT ayeshaiqbal serummetabolomicprofilesforbreastcancerdiagnosisgradingandstagingbygaschromatographymassspectrometry
AT fouziashaikh serummetabolomicprofilesforbreastcancerdiagnosisgradingandstagingbygaschromatographymassspectrometry
AT saleemsomroo serummetabolomicprofilesforbreastcancerdiagnosisgradingandstagingbygaschromatographymassspectrometry
AT syedghulammusharraf serummetabolomicprofilesforbreastcancerdiagnosisgradingandstagingbygaschromatographymassspectrometry
_version_ 1718394308501962752