Iron(II) phthalocyanine Loaded and AS1411 Aptamer Targeting Nanoparticles: A Nanocomplex for Dual Modal Imaging and Photothermal Therapy of Breast Cancer

Yubei He,1 Mengzhu Wang,2 Ming Fu,2 Xun Yuan,3 Yuanli Luo,2 Bin Qiao,2 Jin Cao,2 Zhigang Wang,2 Lan Hao,2,* Gengbiao Yuan1,* 1Department of Nuclear Medicine, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People’s Republic of China; 2Chongqing Key Laborat...

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Autores principales:	He Y, Wang M, Fu M, Yuan X, Luo Y, Qiao B, Cao J, Wang Z, Hao L, Yuan G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	the poly (lactic-co-glycolic) acid theranostics phase transformation photoacoustic imaging ultrasound imaging near infrared Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d55bde93d4e6458f9689eb2a7a46b786
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spelling	oai:doaj.org-article:d55bde93d4e6458f9689eb2a7a46b7862021-12-02T10:31:12ZIron(II) phthalocyanine Loaded and AS1411 Aptamer Targeting Nanoparticles: A Nanocomplex for Dual Modal Imaging and Photothermal Therapy of Breast Cancer1178-2013https://doaj.org/article/d55bde93d4e6458f9689eb2a7a46b7862020-08-01T00:00:00Zhttps://www.dovepress.com/ironii-phthalocyanine-loaded-and-as1411-aptamer-targeting-nanoparticle-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yubei He,1 Mengzhu Wang,2 Ming Fu,2 Xun Yuan,3 Yuanli Luo,2 Bin Qiao,2 Jin Cao,2 Zhigang Wang,2 Lan Hao,2,* Gengbiao Yuan1,* 1Department of Nuclear Medicine, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People’s Republic of China; 2Chongqing Key Laboratory of Ultrasound Molecular Imaging, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People’s Republic of China; 3Department of Ophthalmology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People’s Republic of China*These authors contributed equally to this workCorrespondence: Lan Hao; Gengbiao Yuan Email lanhao5@cqmu.edu.cn; 300784@hospital.cqmu.edu.cnPurpose: A multi-functional nanoplatform with diagnostic imaging and targeted treatment functions has aroused much interest in the nanomedical research field and has been paid more attention in the field of tumor diagnosis and treatment. However, some existing nano-contrast agents have encountered difficulties in different aspects during clinical promotion, such as complicated preparation process and low specificity. Therefore, it is urgent to find a nanocomplex with good targeting effect, high biocompatibility and significant therapeutic effect for the integration of diagnosis and treatment and clinical transformation.Materials and Methods: Nanoparticles (NPs) targeting breast cancer were synthesized by phacoemulsiﬁcation which had liquid ﬂuorocarbon perﬂuoropentane(PFP) in the core and were loaded with Iron(II) phthalocyanine (FePc) on the shell. The aptamer (APT) AS1411 was outside the shell used as a molecular probe. Basic characterization and targeting abilities of the NPs were tested, and their cytotoxicity and biological safety in vivo were evaluated through CCK-8 assay and blood bio-chemical analysis. The photoacoustic (PA) and ultrasound (US) imaging system were used to assess the effects of AS1411-PLGA@FePc@PFP (A-FP NPs) as dual modal contrast agent in vitro and in vivo. The effects of photothermal therapy (PTT) in vitro and in vivo were evaluated through MCF-7 cells and tumor-bearing nude mouse models.Results: A-FP NPs, with good stability, great biocompatibility and low toxicity, were of 201.87 ± 1.60 nm in diameter, and have an active targeting effect on breast cancer cells and tissues. With the help of PA/US imaging, it was proved to be an excellent dual modal contrast agent for diagnosis and guidance of targeted therapy. Meanwhile, it can heat up under near-infrared (NIR) laser irradiation and has achieved obvious antitumor effect both in vitro and in vivo experiments.Conclusion: As a kind of nanomedicine, A-FP NPs can be used in the integration of diagnosis and treatment. The treatment effects and biocompatibility in vivo may provide new thoughts in the clinical transformation of nanomedicine and early diagnosis and treatment of breast cancer.Keywords: poly (lactic-co-glycolic) acid, theranostics, phase transition, photoacoustic imaging, ultrasound imaging, near infraredHe YWang MFu MYuan XLuo YQiao BCao JWang ZHao LYuan GDove Medical Pressarticlethe poly (lactic-co-glycolic) acidtheranosticsphase transformationphotoacoustic imagingultrasound imagingnear infraredMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 5927-5949 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	the poly (lactic-co-glycolic) acid theranostics phase transformation photoacoustic imaging ultrasound imaging near infrared Medicine (General) R5-920
spellingShingle	the poly (lactic-co-glycolic) acid theranostics phase transformation photoacoustic imaging ultrasound imaging near infrared Medicine (General) R5-920 He Y Wang M Fu M Yuan X Luo Y Qiao B Cao J Wang Z Hao L Yuan G Iron(II) phthalocyanine Loaded and AS1411 Aptamer Targeting Nanoparticles: A Nanocomplex for Dual Modal Imaging and Photothermal Therapy of Breast Cancer
description	Yubei He,1 Mengzhu Wang,2 Ming Fu,2 Xun Yuan,3 Yuanli Luo,2 Bin Qiao,2 Jin Cao,2 Zhigang Wang,2 Lan Hao,2,* Gengbiao Yuan1,* 1Department of Nuclear Medicine, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People’s Republic of China; 2Chongqing Key Laboratory of Ultrasound Molecular Imaging, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People’s Republic of China; 3Department of Ophthalmology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People’s Republic of China*These authors contributed equally to this workCorrespondence: Lan Hao; Gengbiao Yuan Email lanhao5@cqmu.edu.cn; 300784@hospital.cqmu.edu.cnPurpose: A multi-functional nanoplatform with diagnostic imaging and targeted treatment functions has aroused much interest in the nanomedical research field and has been paid more attention in the field of tumor diagnosis and treatment. However, some existing nano-contrast agents have encountered difficulties in different aspects during clinical promotion, such as complicated preparation process and low specificity. Therefore, it is urgent to find a nanocomplex with good targeting effect, high biocompatibility and significant therapeutic effect for the integration of diagnosis and treatment and clinical transformation.Materials and Methods: Nanoparticles (NPs) targeting breast cancer were synthesized by phacoemulsiﬁcation which had liquid ﬂuorocarbon perﬂuoropentane(PFP) in the core and were loaded with Iron(II) phthalocyanine (FePc) on the shell. The aptamer (APT) AS1411 was outside the shell used as a molecular probe. Basic characterization and targeting abilities of the NPs were tested, and their cytotoxicity and biological safety in vivo were evaluated through CCK-8 assay and blood bio-chemical analysis. The photoacoustic (PA) and ultrasound (US) imaging system were used to assess the effects of AS1411-PLGA@FePc@PFP (A-FP NPs) as dual modal contrast agent in vitro and in vivo. The effects of photothermal therapy (PTT) in vitro and in vivo were evaluated through MCF-7 cells and tumor-bearing nude mouse models.Results: A-FP NPs, with good stability, great biocompatibility and low toxicity, were of 201.87 ± 1.60 nm in diameter, and have an active targeting effect on breast cancer cells and tissues. With the help of PA/US imaging, it was proved to be an excellent dual modal contrast agent for diagnosis and guidance of targeted therapy. Meanwhile, it can heat up under near-infrared (NIR) laser irradiation and has achieved obvious antitumor effect both in vitro and in vivo experiments.Conclusion: As a kind of nanomedicine, A-FP NPs can be used in the integration of diagnosis and treatment. The treatment effects and biocompatibility in vivo may provide new thoughts in the clinical transformation of nanomedicine and early diagnosis and treatment of breast cancer.Keywords: poly (lactic-co-glycolic) acid, theranostics, phase transition, photoacoustic imaging, ultrasound imaging, near infrared
format	article
author	He Y Wang M Fu M Yuan X Luo Y Qiao B Cao J Wang Z Hao L Yuan G
author_facet	He Y Wang M Fu M Yuan X Luo Y Qiao B Cao J Wang Z Hao L Yuan G
author_sort	He Y
title	Iron(II) phthalocyanine Loaded and AS1411 Aptamer Targeting Nanoparticles: A Nanocomplex for Dual Modal Imaging and Photothermal Therapy of Breast Cancer
title_short	Iron(II) phthalocyanine Loaded and AS1411 Aptamer Targeting Nanoparticles: A Nanocomplex for Dual Modal Imaging and Photothermal Therapy of Breast Cancer
title_full	Iron(II) phthalocyanine Loaded and AS1411 Aptamer Targeting Nanoparticles: A Nanocomplex for Dual Modal Imaging and Photothermal Therapy of Breast Cancer
title_fullStr	Iron(II) phthalocyanine Loaded and AS1411 Aptamer Targeting Nanoparticles: A Nanocomplex for Dual Modal Imaging and Photothermal Therapy of Breast Cancer
title_full_unstemmed	Iron(II) phthalocyanine Loaded and AS1411 Aptamer Targeting Nanoparticles: A Nanocomplex for Dual Modal Imaging and Photothermal Therapy of Breast Cancer
title_sort	iron(ii) phthalocyanine loaded and as1411 aptamer targeting nanoparticles: a nanocomplex for dual modal imaging and photothermal therapy of breast cancer
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/d55bde93d4e6458f9689eb2a7a46b786
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Iron(II) phthalocyanine Loaded and AS1411 Aptamer Targeting Nanoparticles: A Nanocomplex for Dual Modal Imaging and Photothermal Therapy of Breast Cancer

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