Crosstalk between diurnal rhythm and water stress reveals an altered primary carbon flux into soluble sugars in drought-treated rice leaves

Abstract Plants retain rhythmic physiological responses when adapting to environmental challenges. However, possible integrations between drought conditions and those responses have not received much focus, especially regarding crop plants, and the relationship between abiotic stress and the diurnal...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Seo-Woo Kim, Sang-Kyu Lee, Hee-Jeong Jeong, Gynheung An, Jong-Seong Jeon, Ki-Hong Jung
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d55fb273789c4817888ae87c861e12db
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d55fb273789c4817888ae87c861e12db
record_format	dspace
spelling	oai:doaj.org-article:d55fb273789c4817888ae87c861e12db2021-12-02T12:30:12ZCrosstalk between diurnal rhythm and water stress reveals an altered primary carbon flux into soluble sugars in drought-treated rice leaves10.1038/s41598-017-08473-12045-2322https://doaj.org/article/d55fb273789c4817888ae87c861e12db2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08473-1https://doaj.org/toc/2045-2322Abstract Plants retain rhythmic physiological responses when adapting to environmental challenges. However, possible integrations between drought conditions and those responses have not received much focus, especially regarding crop plants, and the relationship between abiotic stress and the diurnal cycle is generally not considered. Therefore, we conducted a genome-wide analysis to identify genes showing both diurnal regulation and water-deficiency response in rice (Oryza sativa). Among the 712 drought-responsive genes primary identified, 56.6% are diurnally expressed while 47.6% of the 761 that are down-regulated by drought are also diurnal. Using the β-glucuronidase reporter system and qRT-PCR analyses, we validated expression patterns of two candidate genes, thereby supporting the reliability of our transcriptome data. MapMan analysis indicated that diurnal genes up-regulated by drought are closely associated with the starch-sucrose pathway while those that are down-regulated are involved in photosynthesis. We then confirmed that starch-sucrose contents and chlorophyll fluorescence are altered in a diurnal manner under drought stress, suggesting these metabolic diurnal alterations as a novel indicator to evaluate the drought response in rice leaves. We constructed a functional gene network associated with the starch-sucrose KEGG metabolic pathway for further functional studies, and also developed a regulatory pathway model that includes OsbZIP23 transcription factor.Seo-Woo KimSang-Kyu LeeHee-Jeong JeongGynheung AnJong-Seong JeonKi-Hong JungNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-18 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seo-Woo Kim Sang-Kyu Lee Hee-Jeong Jeong Gynheung An Jong-Seong Jeon Ki-Hong Jung Crosstalk between diurnal rhythm and water stress reveals an altered primary carbon flux into soluble sugars in drought-treated rice leaves
description	Abstract Plants retain rhythmic physiological responses when adapting to environmental challenges. However, possible integrations between drought conditions and those responses have not received much focus, especially regarding crop plants, and the relationship between abiotic stress and the diurnal cycle is generally not considered. Therefore, we conducted a genome-wide analysis to identify genes showing both diurnal regulation and water-deficiency response in rice (Oryza sativa). Among the 712 drought-responsive genes primary identified, 56.6% are diurnally expressed while 47.6% of the 761 that are down-regulated by drought are also diurnal. Using the β-glucuronidase reporter system and qRT-PCR analyses, we validated expression patterns of two candidate genes, thereby supporting the reliability of our transcriptome data. MapMan analysis indicated that diurnal genes up-regulated by drought are closely associated with the starch-sucrose pathway while those that are down-regulated are involved in photosynthesis. We then confirmed that starch-sucrose contents and chlorophyll fluorescence are altered in a diurnal manner under drought stress, suggesting these metabolic diurnal alterations as a novel indicator to evaluate the drought response in rice leaves. We constructed a functional gene network associated with the starch-sucrose KEGG metabolic pathway for further functional studies, and also developed a regulatory pathway model that includes OsbZIP23 transcription factor.
format	article
author	Seo-Woo Kim Sang-Kyu Lee Hee-Jeong Jeong Gynheung An Jong-Seong Jeon Ki-Hong Jung
author_facet	Seo-Woo Kim Sang-Kyu Lee Hee-Jeong Jeong Gynheung An Jong-Seong Jeon Ki-Hong Jung
author_sort	Seo-Woo Kim
title	Crosstalk between diurnal rhythm and water stress reveals an altered primary carbon flux into soluble sugars in drought-treated rice leaves
title_short	Crosstalk between diurnal rhythm and water stress reveals an altered primary carbon flux into soluble sugars in drought-treated rice leaves
title_full	Crosstalk between diurnal rhythm and water stress reveals an altered primary carbon flux into soluble sugars in drought-treated rice leaves
title_fullStr	Crosstalk between diurnal rhythm and water stress reveals an altered primary carbon flux into soluble sugars in drought-treated rice leaves
title_full_unstemmed	Crosstalk between diurnal rhythm and water stress reveals an altered primary carbon flux into soluble sugars in drought-treated rice leaves
title_sort	crosstalk between diurnal rhythm and water stress reveals an altered primary carbon flux into soluble sugars in drought-treated rice leaves
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d55fb273789c4817888ae87c861e12db
work_keys_str_mv	AT seowookim crosstalkbetweendiurnalrhythmandwaterstressrevealsanalteredprimarycarbonfluxintosolublesugarsindroughttreatedriceleaves AT sangkyulee crosstalkbetweendiurnalrhythmandwaterstressrevealsanalteredprimarycarbonfluxintosolublesugarsindroughttreatedriceleaves AT heejeongjeong crosstalkbetweendiurnalrhythmandwaterstressrevealsanalteredprimarycarbonfluxintosolublesugarsindroughttreatedriceleaves AT gynheungan crosstalkbetweendiurnalrhythmandwaterstressrevealsanalteredprimarycarbonfluxintosolublesugarsindroughttreatedriceleaves AT jongseongjeon crosstalkbetweendiurnalrhythmandwaterstressrevealsanalteredprimarycarbonfluxintosolublesugarsindroughttreatedriceleaves AT kihongjung crosstalkbetweendiurnalrhythmandwaterstressrevealsanalteredprimarycarbonfluxintosolublesugarsindroughttreatedriceleaves
_version_	1718394427368538112

Crosstalk between diurnal rhythm and water stress reveals an altered primary carbon flux into soluble sugars in drought-treated rice leaves

Ejemplares similares