Cryo-EM Structures of CusA Reveal a Mechanism of Metal-Ion Export

The bacterial RND superfamily of efflux pumps mediate resistance to a variety of biocides, including Cu(I) and Ag(I) ions. Here we report four cryo-EM structures of the trimeric CusA pump in the presence of Cu(I). Combined with MD simulations, our data indicate that each CusA protomer within the tr...

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Autores principales:	Mitchell A. Moseng, Meinan Lyu, Tanadet Pipatpolkai, Przemyslaw Glaza, Corey C. Emerson, Phoebe L. Stewart, Phillip J. Stansfeld, Edward W. Yu
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d5623e1c81b943f792bc80254cdda644
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spelling	oai:doaj.org-article:d5623e1c81b943f792bc80254cdda6442021-11-03T18:56:09ZCryo-EM Structures of CusA Reveal a Mechanism of Metal-Ion Export2150-751110.1128/mBio.00452-21https://doaj.org/article/d5623e1c81b943f792bc80254cdda6442021-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00452-21https://doaj.org/toc/2150-7511 The bacterial RND superfamily of efflux pumps mediate resistance to a variety of biocides, including Cu(I) and Ag(I) ions. Here we report four cryo-EM structures of the trimeric CusA pump in the presence of Cu(I). Combined with MD simulations, our data indicate that each CusA protomer within the trimer recognizes and extrudes Cu(I) independently.Mitchell A. MosengMeinan LyuTanadet PipatpolkaiPrzemyslaw GlazaCorey C. EmersonPhoebe L. StewartPhillip J. StansfeldEdward W. YuAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 12, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Mitchell A. Moseng Meinan Lyu Tanadet Pipatpolkai Przemyslaw Glaza Corey C. Emerson Phoebe L. Stewart Phillip J. Stansfeld Edward W. Yu Cryo-EM Structures of CusA Reveal a Mechanism of Metal-Ion Export
description	The bacterial RND superfamily of efflux pumps mediate resistance to a variety of biocides, including Cu(I) and Ag(I) ions. Here we report four cryo-EM structures of the trimeric CusA pump in the presence of Cu(I). Combined with MD simulations, our data indicate that each CusA protomer within the trimer recognizes and extrudes Cu(I) independently.
format	article
author	Mitchell A. Moseng Meinan Lyu Tanadet Pipatpolkai Przemyslaw Glaza Corey C. Emerson Phoebe L. Stewart Phillip J. Stansfeld Edward W. Yu
author_facet	Mitchell A. Moseng Meinan Lyu Tanadet Pipatpolkai Przemyslaw Glaza Corey C. Emerson Phoebe L. Stewart Phillip J. Stansfeld Edward W. Yu
author_sort	Mitchell A. Moseng
title	Cryo-EM Structures of CusA Reveal a Mechanism of Metal-Ion Export
title_short	Cryo-EM Structures of CusA Reveal a Mechanism of Metal-Ion Export
title_full	Cryo-EM Structures of CusA Reveal a Mechanism of Metal-Ion Export
title_fullStr	Cryo-EM Structures of CusA Reveal a Mechanism of Metal-Ion Export
title_full_unstemmed	Cryo-EM Structures of CusA Reveal a Mechanism of Metal-Ion Export
title_sort	cryo-em structures of cusa reveal a mechanism of metal-ion export
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/d5623e1c81b943f792bc80254cdda644
work_keys_str_mv	AT mitchellamoseng cryoemstructuresofcusarevealamechanismofmetalionexport AT meinanlyu cryoemstructuresofcusarevealamechanismofmetalionexport AT tanadetpipatpolkai cryoemstructuresofcusarevealamechanismofmetalionexport AT przemyslawglaza cryoemstructuresofcusarevealamechanismofmetalionexport AT coreycemerson cryoemstructuresofcusarevealamechanismofmetalionexport AT phoebelstewart cryoemstructuresofcusarevealamechanismofmetalionexport AT phillipjstansfeld cryoemstructuresofcusarevealamechanismofmetalionexport AT edwardwyu cryoemstructuresofcusarevealamechanismofmetalionexport
_version_	1718445486327726080

Cryo-EM Structures of CusA Reveal a Mechanism of Metal-Ion Export

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