Isolation, culture, and immunostaining of neonatal rat ventricular myocytes

Summary: Isolation and culture of ventricular cardiomyocytes from neonatal rats (NRVMs) is a powerful model to study neonatal cardiac development, cell cycle regulation, and cardiac physiology and pathology in vitro. Here, we present our modified enzymatic digestion protocol followed by two-step dis...

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Autores principales:	Ana Helena Macedo Pereira, Alisson Campos Cardoso, Kleber Gomes Franchini
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell Biology Cell culture Cell isolation Microscopy Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/d56868389da244d3a46b14fee40b3745
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spelling	oai:doaj.org-article:d56868389da244d3a46b14fee40b37452021-11-14T04:35:37ZIsolation, culture, and immunostaining of neonatal rat ventricular myocytes2666-166710.1016/j.xpro.2021.100950https://doaj.org/article/d56868389da244d3a46b14fee40b37452021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006560https://doaj.org/toc/2666-1667Summary: Isolation and culture of ventricular cardiomyocytes from neonatal rats (NRVMs) is a powerful model to study neonatal cardiac development, cell cycle regulation, and cardiac physiology and pathology in vitro. Here, we present our modified enzymatic digestion protocol followed by two-step discontinuous Percoll gradient centrifugation to isolate a high yield of viable ventricular cardiomyocytes from neonatal rats. Finally, here we describe an immunostaining protocol for cytosolic and nuclear staining of NRVMs.For complete details on the use and execution of this protocol, please refer to Pereira et al. (2020).Ana Helena Macedo PereiraAlisson Campos CardosoKleber Gomes FranchiniElsevierarticleCell BiologyCell cultureCell isolationMicroscopyScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100950- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell Biology Cell culture Cell isolation Microscopy Science (General) Q1-390
spellingShingle	Cell Biology Cell culture Cell isolation Microscopy Science (General) Q1-390 Ana Helena Macedo Pereira Alisson Campos Cardoso Kleber Gomes Franchini Isolation, culture, and immunostaining of neonatal rat ventricular myocytes
description	Summary: Isolation and culture of ventricular cardiomyocytes from neonatal rats (NRVMs) is a powerful model to study neonatal cardiac development, cell cycle regulation, and cardiac physiology and pathology in vitro. Here, we present our modified enzymatic digestion protocol followed by two-step discontinuous Percoll gradient centrifugation to isolate a high yield of viable ventricular cardiomyocytes from neonatal rats. Finally, here we describe an immunostaining protocol for cytosolic and nuclear staining of NRVMs.For complete details on the use and execution of this protocol, please refer to Pereira et al. (2020).
format	article
author	Ana Helena Macedo Pereira Alisson Campos Cardoso Kleber Gomes Franchini
author_facet	Ana Helena Macedo Pereira Alisson Campos Cardoso Kleber Gomes Franchini
author_sort	Ana Helena Macedo Pereira
title	Isolation, culture, and immunostaining of neonatal rat ventricular myocytes
title_short	Isolation, culture, and immunostaining of neonatal rat ventricular myocytes
title_full	Isolation, culture, and immunostaining of neonatal rat ventricular myocytes
title_fullStr	Isolation, culture, and immunostaining of neonatal rat ventricular myocytes
title_full_unstemmed	Isolation, culture, and immunostaining of neonatal rat ventricular myocytes
title_sort	isolation, culture, and immunostaining of neonatal rat ventricular myocytes
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/d56868389da244d3a46b14fee40b3745
work_keys_str_mv	AT anahelenamacedopereira isolationcultureandimmunostainingofneonatalratventricularmyocytes AT alissoncamposcardoso isolationcultureandimmunostainingofneonatalratventricularmyocytes AT klebergomesfranchini isolationcultureandimmunostainingofneonatalratventricularmyocytes
_version_	1718429927437500416

Isolation, culture, and immunostaining of neonatal rat ventricular myocytes

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