OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice (<i>Oryza sativa</i> L.)

Gibberellins (GAs) are diterpenoid phytohormones regulating various aspects of plant growth and development, such as internode elongation and seed germination. Although the GA biosynthesis pathways have been identified, the transcriptional regulatory network of GA homeostasis still remains elusive....

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Autores principales:	Liqun Tang, Huayu Xu, Yifeng Wang, Huimei Wang, Zhiyong Li, Xixi Liu, Yazhou Shu, Guan Li, Wanning Liu, Jiezheng Ying, Xiaohong Tong, Jialing Yao, Wenfei Xiao, Shaoqing Tang, Shen Ni, Jian Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	rice (<i>Oryza sativa</i> L.) gibberellins semi-dwarf seed germination polycomb repression complex bZIP Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/d569d011426e4ab781dd777531888d71
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spelling	oai:doaj.org-article:d569d011426e4ab781dd777531888d712021-11-25T17:54:27ZOsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice (<i>Oryza sativa</i> L.)10.3390/ijms2222122201422-00671661-6596https://doaj.org/article/d569d011426e4ab781dd777531888d712021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12220https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Gibberellins (GAs) are diterpenoid phytohormones regulating various aspects of plant growth and development, such as internode elongation and seed germination. Although the GA biosynthesis pathways have been identified, the transcriptional regulatory network of GA homeostasis still remains elusive. Here, we report the functional characterization of a GA-inducible <i>OsABF1</i> in GA biosynthesis underpinning plant height and seed germination. Overexpression of <i>OsABF1</i> produced a typical GA-deficient phenotype with semi-dwarf and retarded seed germination. Meanwhile, the phenotypes could be rescued by exogenous GA<sub>3</sub>, suggesting that <i>OsABF1</i> is a key regulator of GA homeostasis. OsABF1 could directly suppress the transcription of green revolution gene <i>SD1</i>, thus reducing the endogenous GA level in rice. Moreover, OsABF1 interacts with and transcriptionally antagonizes to the polycomb repression complex component OsEMF2b, whose mutant showed as similar but more severe phenotype to <i>OsABF1</i> overexpression lines. It is suggested that OsABF1 recruits RRC2-mediated H3K27me3 deposition on the <i>SD1</i> promoter, thus epigenetically silencing <i>SD1</i> to maintain the GA homeostasis for growth and seed germination. These findings shed new insight into the functions of <i>OsABF1</i> and regulatory mechanism underlying GA homeostasis in rice.Liqun TangHuayu XuYifeng WangHuimei WangZhiyong LiXixi LiuYazhou ShuGuan LiWanning LiuJiezheng YingXiaohong TongJialing YaoWenfei XiaoShaoqing TangShen NiJian ZhangMDPI AGarticlerice (<i>Oryza sativa</i> L.)gibberellinssemi-dwarfseed germinationpolycomb repression complexbZIPBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12220, p 12220 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rice (<i>Oryza sativa</i> L.) gibberellins semi-dwarf seed germination polycomb repression complex bZIP Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	rice (<i>Oryza sativa</i> L.) gibberellins semi-dwarf seed germination polycomb repression complex bZIP Biology (General) QH301-705.5 Chemistry QD1-999 Liqun Tang Huayu Xu Yifeng Wang Huimei Wang Zhiyong Li Xixi Liu Yazhou Shu Guan Li Wanning Liu Jiezheng Ying Xiaohong Tong Jialing Yao Wenfei Xiao Shaoqing Tang Shen Ni Jian Zhang OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice (<i>Oryza sativa</i> L.)
description	Gibberellins (GAs) are diterpenoid phytohormones regulating various aspects of plant growth and development, such as internode elongation and seed germination. Although the GA biosynthesis pathways have been identified, the transcriptional regulatory network of GA homeostasis still remains elusive. Here, we report the functional characterization of a GA-inducible <i>OsABF1</i> in GA biosynthesis underpinning plant height and seed germination. Overexpression of <i>OsABF1</i> produced a typical GA-deficient phenotype with semi-dwarf and retarded seed germination. Meanwhile, the phenotypes could be rescued by exogenous GA<sub>3</sub>, suggesting that <i>OsABF1</i> is a key regulator of GA homeostasis. OsABF1 could directly suppress the transcription of green revolution gene <i>SD1</i>, thus reducing the endogenous GA level in rice. Moreover, OsABF1 interacts with and transcriptionally antagonizes to the polycomb repression complex component OsEMF2b, whose mutant showed as similar but more severe phenotype to <i>OsABF1</i> overexpression lines. It is suggested that OsABF1 recruits RRC2-mediated H3K27me3 deposition on the <i>SD1</i> promoter, thus epigenetically silencing <i>SD1</i> to maintain the GA homeostasis for growth and seed germination. These findings shed new insight into the functions of <i>OsABF1</i> and regulatory mechanism underlying GA homeostasis in rice.
format	article
author	Liqun Tang Huayu Xu Yifeng Wang Huimei Wang Zhiyong Li Xixi Liu Yazhou Shu Guan Li Wanning Liu Jiezheng Ying Xiaohong Tong Jialing Yao Wenfei Xiao Shaoqing Tang Shen Ni Jian Zhang
author_facet	Liqun Tang Huayu Xu Yifeng Wang Huimei Wang Zhiyong Li Xixi Liu Yazhou Shu Guan Li Wanning Liu Jiezheng Ying Xiaohong Tong Jialing Yao Wenfei Xiao Shaoqing Tang Shen Ni Jian Zhang
author_sort	Liqun Tang
title	OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice (<i>Oryza sativa</i> L.)
title_short	OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice (<i>Oryza sativa</i> L.)
title_full	OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice (<i>Oryza sativa</i> L.)
title_fullStr	OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice (<i>Oryza sativa</i> L.)
title_full_unstemmed	OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice (<i>Oryza sativa</i> L.)
title_sort	osabf1 represses gibberellin biosynthesis to regulate plant height and seed germination in rice (<i>oryza sativa</i> l.)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d569d011426e4ab781dd777531888d71
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OsABF1 Represses Gibberellin Biosynthesis to Regulate Plant Height and Seed Germination in Rice (<i>Oryza sativa</i> L.)

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